A5024464260- Axon Guidance and Neuronal Signaling
- Apelin-related biomedical research
- Advanced Fluorescence Microscopy Techniques
- Cancer Cells and Metastasis
- Cancer Mechanisms and Therapy
- Immunotherapy and Immune Responses
- Allergic Rhinitis and Sensitization
- Cell Image Analysis Techniques
- Angiogenesis and VEGF in Cancer
- Terahertz technology and applications
- Photoacoustic and Ultrasonic Imaging
- Single-cell and spatial transcriptomics
- Retinal Diseases and Treatments
- Food Allergy and Anaphylaxis Research
- PI3K/AKT/mTOR signaling in cancer
- Contact Dermatitis and Allergies
- 3D Printing in Biomedical Research
- T-cell and B-cell Immunology
- Retinal Development and Disorders
- Optical Coherence Tomography Applications
- Respiratory Support and Mechanisms
- Optical Imaging and Spectroscopy Techniques
- Pancreatic function and diabetes
- Sepsis Diagnosis and Treatment
- Vascular Malformations Diagnosis and Treatment
St. Vincent's Hospital
2024
Massachusetts General Hospital
2022
Korea Advanced Institute of Science and Technology
2013-2021
Vanderbilt University Medical Center
2019
Center for NanoScience
2017
Kootenay Association for Science & Technology
2013
The lung is highly vulnerable during sepsis, yet its functional deterioration accompanied by disturbances in the pulmonary microcirculation poorly understood. This study aimed to investigate how distorted sepsis-induced acute injury (ALI) and reveal underlying cellular pathophysiologic mechanism. Using a custom-made intravital microscopic imaging system murine model of ALI, we achieved direct real-time visualisation circulating cells vivo . We derived capillary ratio (FCR) as quantitative...
Schlemm’s canal (SC) is a specialized vascular structure in the eye that functions to drain aqueous humor from intraocular chamber into systemic circulation. Dysfunction of SC has been proposed underlie increased outflow (AHO) resistance, which leads elevated ocular pressure, factor for glaucoma development humans. Here, using lymphatic and blood vasculature reporter mice, we determined SC, originates vessels during postnatal period, acquires identity through upregulation prospero homeobox...
In the central nervous system, endothelial cells (ECs) and pericytes (PCs) of blood vessel walls cooperatively form a physical chemical barrier to maintain neural homeostasis. However, in diabetic retinopathy (DR), loss PCs from is assumed cause breakdown blood-retina (BRB) subsequent vision-threatening vascular dysfunctions. Nonetheless, lack adequate DR animal models has precluded disease understanding drug discovery. Here, by using an anti-PDGFRβ antibody, we show that transient...
Angpt-Tie2 is essential for choriocapillary maintenance, and Tie2 activation could be a therapeutic avenue fundamental treatment of NV-AMD.
The recent development of THz sources in a wide range frequencies and power levels has led to greatly increased interest potential biomedical applications such as cancer burn wound diagnosis. However, despite its importance realizing wave based applications, our knowledge how irradiation can affect live tissue at the cellular level is very limited. In this study, an acute inflammatory response caused by pulsed on skin mouse was analyzed using intravital laser-scanning confocal microscopy....
The purpose of this study was to evaluate the performance optical coherence tomography angiography (OCTA) in visualizing laser-induced choroidal neovascularization (CNV) rodent retina.Choroidal induced via laser photocoagulation 2 male Brown Norway rats and C57BL/6 mice. For qualitative comparison, animals were imaged vivo with OCTA, indocyanine green (ICGA), fluorescein (FA), ex immunofluorescence confocal microscopy, 14 days post without anti-vascular endothelial growth factor (anti-VEGF)...
The number of circulating tumor cell (CTC) in the peripheral blood cancer patients can be a valuable biomarker for diagnosis and treatment monitoring. In this study, we implemented custom-design video-rate confocal microscopy system capable direct visualization fast flowing CTC at great saphenous vein (GSV) live animal model vivo. Continuous acquisition images GSV revealed highly dynamic time-dependent changes intravenously injected cells. By extracting calibration factor through...
Direct intravital imaging of an endothelial surface layer (ESL) in pulmonary microcirculation could be a valuable approach to investigate the role vascular barrier various pathological conditions.Despite its importance as marker cell damage and impairment system, vivo visualization ESL has remained challenging technical issue.In this work, we implemented system integrated custom-design video-rate laser scanning confocal microscopy platform.Using real-time cellular-level microscopic lung was...
The lymph node (LN) is the primary site of alloimmunity activation and regulation during transplantation. Here, we investigated how fibroblastic reticular cells (FRCs) facilitate tolerance induced by anti-CD40L in a murine model heart We found that both absence LNs FRC depletion abrogated effect prolonging allograft survival. Depletion FRCs impaired homing T across high endothelial venules (HEVs) promoted formation alloreactive heart-transplanted mice treated with anti-CD40L. Single-cell RNA...
Lymph node (LN) is an important immune organ that controls adaptive responses against foreign pathogens and abnormal cells. To facilitate efficient function, LN has highly organized 3D cellular structures, vascular lymphatic system. Unfortunately, conventional histological analysis relying on thin-sliced tissue limitations in due to structural disruption loss the processes of fixation slicing. Optical sectioning confocal microscopy been utilized analyze structure intact without physical...
Lymph nodes (LN) are major checkpoints for circulating T lymphocytes to recognize foreign antigens collected from peripheral tissue. High endothelial venule (HEV) in LN facilitates the effective transmigration of blood into LN. There have been many efforts visualize trafficking across HEV understand underlying mechanism. However, due insufficient spatiotemporal resolution and lack an vivo labeling method, clear visualization dynamic behaviors rapidly flowing their difficult. In this work, we...
Indocyanine green (ICG) is a near-infrared fluorophore approved for human use which has been widely used various clinical applications. Despite the well-established usage, our understanding about microscopic in vivo pharmacokinetics of systemically administered ICG relatively limited. In this work, we successfully visualized real-time pharmacokinetic dynamics intravenously injected free-form and liposomal cellular resolution by utilizing custom-built video-rate near infrared laser-scanning...
Highly angiogenic bone marrow mononuclear cell–derived spheroids (BM-spheroids), formed by selective proliferation of the CD31+CD14+CD34+ monocyte subset via three-dimensional (3D) culture, have had robust angiogenetic capacity in rodent syngeneic renal subcapsular islet transplantation. We wondered whether efficacy BM-spheroids could be demonstrated clinically relevant intraportal transplantation models without increasing risk portal thrombosis. The thrombogenic potential intraportally...
Longitudinal imaging of murine pancreas is technically challenging due to the mechanical softness tissue influenced by peristalsis. Here, we report a novel pancreatic window for long-term stabilized cellular-level observation islets in vivo. By spatially separating from bowel movement and physiologic respiration with metal plate integrated window, successfully tracked up three weeks visualized dumbbell-shape transformation single islet. This can be useful tool visualization microstructure...
Visualization of cellular dynamics in the gastrointestinal tract living mouse model to investigate pathophysiology has been a long-pursuing goal. Especially, for chronic disease such as Crohn's disease, longitudinal observation luminal surface small intestine single is highly desirable complex pathogenesis sequential time points. In this work, by utilizing micro-GRIN lens based side-view endomicroscope integrated into video-rate confocal microscopy system, we successfully performed...
The purpose of this study was to evaluate longitudinal gene expression patterns by retinal imaging using a modified custom-built confocal laser-scanning microscope in experimental rats after intravitreal injection recombinant adeno-associated virus 2 (rAAV2-green fluorescent protein [GFP]).Ten 9-week-old Wistar were divided into two groups: group (group 1) that received rAAV2-GFP and control 2) vehicle. After anesthesia Zoletil intraperitoneal injection, 8 μL 1 or vehicle injected...
Background Causes of pediatric pollen food allergy syndrome (PFAS) differ depending on airborne levels in a particular region. We aimed to analyze counts, IgE sensitization rates, and PFAS incidence among children with allergies South Korea Japan. Methods This cross-sectional study included aged 5–17 years 2017. Airborne samples were collected from Busan Korea, Fukuoka Tochigi Questionnaires used assess bronchial asthma, seasonal allergic rhinitis, atopic dermatitis, allergy, PFAS. The serum...
The purpose of this study was to identify the causes, symptoms, and complications hypoproteinemia prevent provide appropriate treatment children with atopic dermatitis.Children diagnosed dermatitis and/or hypoalbuminemia were retrospectively reviewed. patients' medical records, including family history, weight, treatment, complications, laboratory test results for allergies skin cultures, examined.Twenty-six patients (24 boys) enrolled. Seven cases had growth retardation; 7,...
In vivo, longitudinal observation of tumorigenesis in a live mouse model over an extended time period has been actively pursued to obtain better understanding the cellular and molecular mechanism highly complex tumor microenvironment. However, common intravital imaging approaches based on conventional laser scanning confocal or two-photon microscope have mostly limited superficial parts solid tissue. this work, we implemented small diameter needle-shaped side-view endomicroscope that can be...
Immunoreactive dynamics of tumor-infiltrating lymphocytes (TILs) within the tumor microenvironment in breast cancer are not well understood. This study aimed to investigate spatiotemporal cellular TILs models. Breast cells were implanted into dorsal skinfold chamber BALB/c nude mice, and T adoptively transferred. Longitudinal intravital imaging was performed, assessed. In 4T1 model, progressively exhibited increased motility, their motility inside significantly higher than that outside...
Abstract Mechanistic target of rapamycin (mTOR) is a serine/threonine kinase that acts in two distinct complexes, mTORC1 and mTORC2, dysregulated many diseases including cancer. mLST8 shared component both yet little known regarding how contributes to assembly activity the mTOR complexes. We assessed loss panel normal cancer cells, finding no impact on activity, nor with co-factor Raptor. However, blocked association mTORC2 co-factors Rictor Sin1, impaired its phosphorylation AKT at S473....
By utilizing custom-design video-rate near-infrared laser-scanning confocal microscope, we imaged the pharmacokinetic dynamics of intravenously injected ICG at skin and liver mouse in sub-cellular scale vivo.