A5103138728- Cancer Cells and Metastasis
- Cell Image Analysis Techniques
- Sepsis Diagnosis and Treatment
- Immunotherapy and Immune Responses
- Advanced Fluorescence Microscopy Techniques
- 3D Printing in Biomedical Research
- Angiogenesis and VEGF in Cancer
- Respiratory Support and Mechanisms
- Microbial Metabolic Engineering and Bioproduction
- Cardiac Ischemia and Reperfusion
- Cell Adhesion Molecules Research
- Nanoplatforms for cancer theranostics
- Cardiac Arrest and Resuscitation
- Photoacoustic and Ultrasonic Imaging
- Atomic and Subatomic Physics Research
- Ultrasound and Hyperthermia Applications
- Anesthesia and Neurotoxicity Research
- Optical Coherence Tomography Applications
- Thermoregulation and physiological responses
- Process Optimization and Integration
- Hematopoietic Stem Cell Transplantation
- Thermal Regulation in Medicine
- Immune Cell Function and Interaction
- Ion Transport and Channel Regulation
- Single-cell and spatial transcriptomics
Korea Advanced Institute of Science and Technology
2013-2022
Kootenay Association for Science & Technology
2013
Korea Research Institute of Chemical Technology
2009
Konkuk University
2008
The lung is highly vulnerable during sepsis, yet its functional deterioration accompanied by disturbances in the pulmonary microcirculation poorly understood. This study aimed to investigate how distorted sepsis-induced acute injury (ALI) and reveal underlying cellular pathophysiologic mechanism. Using a custom-made intravital microscopic imaging system murine model of ALI, we achieved direct real-time visualisation circulating cells vivo . We derived capillary ratio (FCR) as quantitative...
Tumor metastasis involves circulating and tumor‐initiating capacities of metastatic cancer cells. Epithelial‐mesenchymal transition (EMT) is related to self‐renewal capacity tumor cell (CTC) characteristics for metastasis. Although a life‐threatening, complicated process that occurs through circulation cells, mechanistic aspects have been largely unknown. Hepatic transmembrane 4 L six family member 5 (TM4SF5) promotes EMT malignant growth migration, so it was rationalized TM4SF5, as...
Transarterial chemoembolization (TACE) is an image-guided locoregional therapy used for the treatment of patients with primary or secondary liver cancer. However, conventional TACE formulations are rapidly dissociated due to instability emulsion, resulting in insufficient local drug concentrations target tumor. Methods: To overcome these limitations, a doxorubicin-loaded albumin nanoparticle-conjugated microbubble complex iodized oil emulsion (DOX-NPs-MB Lipiodol) has been developed as new...
The number of circulating tumor cell (CTC) in the peripheral blood cancer patients can be a valuable biomarker for diagnosis and treatment monitoring. In this study, we implemented custom-design video-rate confocal microscopy system capable direct visualization fast flowing CTC at great saphenous vein (GSV) live animal model vivo. Continuous acquisition images GSV revealed highly dynamic time-dependent changes intravenously injected cells. By extracting calibration factor through...
Abstract Current nanoparticle (NP) drug carriers mostly depend on the enhanced permeability and retention (EPR) effect for selective delivery to solid tumors. However, in absence of a persistent EPR effect, peritumoral endothelium can function as an access barrier tumors negatively affect effectiveness NPs. In recognition potential tumors, poly(lactic‐co‐glycolic acid) (PLGA) NPs are modified with quinic acid (QA) derivative, synthetic mimic selectin ligands. QA‐decorated (QA‐NP) interact...
Direct intravital imaging of an endothelial surface layer (ESL) in pulmonary microcirculation could be a valuable approach to investigate the role vascular barrier various pathological conditions.Despite its importance as marker cell damage and impairment system, vivo visualization ESL has remained challenging technical issue.In this work, we implemented system integrated custom-design video-rate laser scanning confocal microscopy platform.Using real-time cellular-level microscopic lung was...
Renewable thermoset elastomers were prepared using the plant-based monomer carvomenthide. Controlled ring-opening transesterification polymerization of carvomenthide diethylene glycol as an initiator gave α,ω-dihydroxyl poly(carvomenthide) (HO-PCM-OH), which was subsequently converted to carboxy-telechelic (HOOC-PCM-COOH) by esterification with excess succinic anhydride through a one-pot, two-step process, leading no crystallinity, high viscosity, strong thermal resistance, and low glass...
Lymph node (LN) is an important immune organ that controls adaptive responses against foreign pathogens and abnormal cells. To facilitate efficient function, LN has highly organized 3D cellular structures, vascular lymphatic system. Unfortunately, conventional histological analysis relying on thin-sliced tissue limitations in due to structural disruption loss the processes of fixation slicing. Optical sectioning confocal microscopy been utilized analyze structure intact without physical...
Lymph nodes (LN) are major checkpoints for circulating T lymphocytes to recognize foreign antigens collected from peripheral tissue. High endothelial venule (HEV) in LN facilitates the effective transmigration of blood into LN. There have been many efforts visualize trafficking across HEV understand underlying mechanism. However, due insufficient spatiotemporal resolution and lack an vivo labeling method, clear visualization dynamic behaviors rapidly flowing their difficult. In this work, we...
Bone marrow is a vital tissue that produces the majority of erythrocytes, thrombocytes, and immune cells. transplantation (BMT) has been widely performed in patients with blood disorders cancers. However, cellular-level behaviors transplanted bone cells over wide-areas host after BMT are not fully understood yet. In this work, we longitudinal wide-area observation calvarial vivo. Using H2B-GFP/β-actin-DsRed double-transgenic mouse model as donor, subcellular-level nuclear-cytoplasmic...
In vivo, longitudinal observation of tumorigenesis in a live mouse model over an extended time period has been actively pursued to obtain better understanding the cellular and molecular mechanism highly complex tumor microenvironment. However, common intravital imaging approaches based on conventional laser scanning confocal or two-photon microscope have mostly limited superficial parts solid tissue. this work, we implemented small diameter needle-shaped side-view endomicroscope that can be...
Immunoreactive dynamics of tumor-infiltrating lymphocytes (TILs) within the tumor microenvironment in breast cancer are not well understood. This study aimed to investigate spatiotemporal cellular TILs models. Breast cells were implanted into dorsal skinfold chamber BALB/c nude mice, and T adoptively transferred. Longitudinal intravital imaging was performed, assessed. In 4T1 model, progressively exhibited increased motility, their motility inside significantly higher than that outside...
By utilizing custom-design high-speed video-rate laser-scanning confocal microscope system for visualizing fast-flowing cells at the great saphenous vein of mouse, we quantitated circulating tumor whole body blood, in vivo, by hemocytometric imaging analysis.
We established an intravital high-speed laser-scanning confocal microscopy capable of visualizing fast-flowing individual cells at great saphenous vein mouse model and quantified circulating tumor whole body blood, in vivo, by image-based analysis.
We present an in vivo lung imaging of murine model for pulmonary disease by using custom-design video-rate laser-scanning confocal microscopy integrated with suction chamber motion stabilization.
Copyright © by 2015 the Society of Critical Care Medicine and Wolters Kluwer Health, Inc. All Rights Reserved.