A5063503016- Microtubule and mitosis dynamics
- Genomics and Chromatin Dynamics
- Epigenetics and DNA Methylation
- DNA Repair Mechanisms
- RNA Research and Splicing
- DNA and Nucleic Acid Chemistry
- Chromosomal and Genetic Variations
- RNA modifications and cancer
- Anesthesia and Neurotoxicity Research
- Cell Image Analysis Techniques
- Pancreatic function and diabetes
- Analytical Methods in Pharmaceuticals
- Nuclear Structure and Function
- 14-3-3 protein interactions
- Hippo pathway signaling and YAP/TAZ
- Cellular Mechanics and Interactions
- Drug-Induced Adverse Reactions
- Effects and risks of endocrine disrupting chemicals
- Carcinogens and Genotoxicity Assessment
- Birth, Development, and Health
- Congenital limb and hand anomalies
- Pharmaceutical studies and practices
University of Science and Technology of China
2012-2024
Harvard University
2018-2024
Hong Kong University of Science and Technology
2024
University of Hong Kong
2024
Morehouse School of Medicine
2021
Center for Excellence in Molecular Cell Science
2019
Hefei National Center for Physical Sciences at Nanoscale
2012-2014
Kings County Hospital Center
1957
York University
1957
State University of New York
1957
Histone methylation performs multiple functions such as DNA replication, transcription regulation, heterochromatin formation, and chromatin condensation. How this gradient is orchestrated in the centromere during chromosome segregation not known. Here we examine temporal dynamics of protein by SUV39H1 methyltransferase, a key mitotic regulator, using fluorescence resonance energy transfer-based sensors living HeLa cells immunofluorescence native substrates. A quantitative analysis dynamics,...
The classical phenomenon of crossover interference is a one-dimensional spatial patterning process that produces evenly spaced crossovers during meiosis. Quantitative analysis diagnostic molecules along budding yeast chromosomes reveals this also sets up second, interdigitated pattern related but longer periodicity, in "two-tiered" process. second tier corresponds to previously mysterious minority set crossovers. Thus, toto, the two tiers account for all detected events. Both spatially...
Dynamic disassembly and reconstruction of the nuclear lamina during entry exit mitosis, respectively, are pivotal steps in proliferation higher eukaryotic cells. Although numerous post-translational modifications lamin proteins have been identified, key factors driving dynamics remain elusive. Here we identified CDK1-elicited phosphorylation sites on endogenous Lamin A/C characterized their functions regulation lamina. Specifically, mass spectrometry revealed CDK1-mediated at N-terminal...
Stable transmission of genetic material during cell division requires accurate chromosome segregation. PLK1 dynamics at kinetochores control establishment correct kinetochore-microtubule attachments and subsequent silencing the spindle checkpoint. However, regulatory mechanism responsible for activity in prometaphase has not yet been affirmatively identified. Here we identify Apolo1, which tunes attachments. Apolo1 localizes to early mitosis, suppression results misaligned chromosomes. Using...
Accurate chromosome segregation during mitosis requires the physical separation of sister chromatids which depends on correct position mitotic spindle relative to membrane cortex. Although recent work has identified role PLK1 in orientation, mechanisms underlying signaling positioning and orientation have not been fully illustrated. Here, we a conserved axis NDR1 kinase activity is regulated by mitosis. phosphorylates at three putative threonine residues (T7, T183 T407) entry, elicits...
Faithful segregation of mitotic chromosomes requires bi-orientation sister chromatids, which relies on the sensing correct attachments between spindle microtubules and kinetochores. Although mechanisms underlying PLK1 activation have been extensively studied, regulatory that couple activity to accurate chromosome are not well understood. In particular, is implicated in stabilizing kinetochore-microtubule attachments, but how kinetochore regulated avoid hyperstabilized...
During mitosis, from late prophase onward, sister chromatids are connected along their entire lengths by axis-linking chromatin/structure bridges. prometaphase/metaphase, these bridges ensure that retain a parallel, paranemic relationship, without helical coiling, as they undergo compaction. Bridges must then be removed during anaphase. Motivated findings, the present study has further investigated process of anaphase separation. Morphological and functional analyses mammalian mitoses reveal...
Heterochromatin protein 1α (HP1α) regulates chromatin specification and plasticity during cell fate decision. Different structural determinants account for HP1α localization function division cycle. Our earlier study showed that centromeric of depends on the epigenetic mark H3K9me3 in interphase, while its location mitosis relies uncharacterized PXVXL-containing factors. Here, we identified a protein, ligand-dependent nuclear receptor-interacting factor 1 (LRIF1), which recruits to...
Significance Spatial patterns are a prominent and interesting feature of both biological physical systems. We have discovered that mammalian mitotic chromosomes, which comprise two closely associated sister chromatids, exhibit spatial patterns. In one pattern, the structural axis each chromatid acquires sequential partial helices alternating handedness. other, an array evenly spaced bridges links these axes. Development any pattern requires communication within system. present constellation...
Histone methylation performs multiple functions such as DNA replication, transcription regulation, heterochromatin formation, and chromatin condensation. How this gradient is orchestrated in the centromere during chromosome segregation not known. Here we examine temporal dynamics of protein by SUV39H1 methyltransferase, a key mitotic regulator, using fluorescence resonance energy transfer‐based sensors living HeLa cells immunofluorescence native substrates. A quantitative analysis dynamics,...