A5015831623- Organ Transplantation Techniques and Outcomes
- Immune Response and Inflammation
- Liver Disease and Transplantation
- NF-κB Signaling Pathways
- Immune cells in cancer
- Hepatocellular Carcinoma Treatment and Prognosis
- Liver Disease Diagnosis and Treatment
- Gallbladder and Bile Duct Disorders
- Pediatric Hepatobiliary Diseases and Treatments
- Drug Transport and Resistance Mechanisms
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Liver physiology and pathology
- Biliary and Gastrointestinal Fistulas
- Pancreatic and Hepatic Oncology Research
- Anesthesia and Neurotoxicity Research
- Cancer-related molecular mechanisms research
- Pancreatitis Pathology and Treatment
- Congenital Anomalies and Fetal Surgery
- Sarcoma Diagnosis and Treatment
- Esophageal Cancer Research and Treatment
- interferon and immune responses
- MicroRNA in disease regulation
- Abdominal vascular conditions and treatments
- Ferroptosis and cancer prognosis
- Inflammation biomarkers and pathways
Dalian Medical University
2015-2024
Chongqing Medical University
2015-2024
Second Affiliated Hospital of Chongqing Medical University
2015-2024
Eastern Hepatobiliary Surgery Hospital
2024
Second Military Medical University
2024
Xuzhou Medical College
2022
Second People’s Hospital of Huai’an
2022
Sichuan University
2008-2020
West China Second University Hospital of Sichuan University
2020
Shanghai Changning Maternity and Infant Health Hospital
2018
Because the role of Kupffer cells (KCs) in liver transplantation (LT) tolerance is not well understood, we investigated their allograft acceptance rats. Male Sprague-Dawley rats were randomly assigned to either an LT group or a pretreated with GdCl3 (Gd group). The postoperatively sacrificed at indicated times for histology and assessment KC function, nuclear factor kappa B (NF-κB) activity, cytokine production. KCs T (TCs) isolated from allografts assess Fas/Fas ligand (FasL) expression....
To investigate the effect and mechanism of macrophage membrane-coated nanoparticles (M-NPs) on hepatic ischemia-reperfusion injury (I/RI) caused by orthotopic liver transplantation. In addition, advantages TLR4+/M-NPs compared to M-NPs are discussed.We prepared biomimetic identified their characteristics. were injected into an SD rat model transplantation, anti-inflammatory anti-I/RI activities studied in vivo vitro. we overexpressed membrane Toll-like receptor 4 (TLR4) vitro TLR4+/M-NPs....
Pyruvate kinase M2 (PKM2) is a key glycolysis enzyme, and its effect on macrophages has not been entirely elucidated. Here, we identified that the PKM2 small-molecule agonist TEPP-46 mediated activation by inducing formation of tetramer promoted macrophage endotoxin tolerance. Lipopolysaccharide (LPS)-tolerant mice had higher expression tetramer, which was associated with reduced in vivo immune response to LPS. Pretreatment resulted tolerance LPS stimulation, as demonstrated significant...
Donation after circulatory death livers are more susceptible to ischemia/reperfusion injury (IRI) because of a longer period warm ischemia. Growing evidence now suggests that ferroptosis plays key regulatory role in the development IRI, so targeting may be an effective strategy alleviate IRI liver transplantation (LT).
The α-ketoglutarate (αKG), a metabolite of glutaminolysis, is reported to orchestrate macrophages activation. This study aims clarify whether the αKG / glutaminolysis metabolism can suppress Kupffer cells (KCs) activation during liver transplantation and attenuate hepatic ischemia-reperfusion injury (IRI).Donor livers were perfused with DM-αKG (a cell-permeable analog αKG) or BPTES (an inhibitor glutaminase 1) via portal vein cold preservation, controls UW solution. Then, rat model was...
Abstract In this study, we aimed to compare and validate the prognostic abilities of preoperative systemic immune cells in hepatocellular carcinoma ( HCC ) after curative hepatectomy. We developed two nomograms predict postoperative recurrence‐free survival RFS overall OS comparisons cell scores. The were constructed based on 305 patients who underwent hepatectomy for . predictive accuracy discriminative ability compared with six commonly used staging systems results validated using...
The objective of this study is to summarize the experience in diagnosis and treatment Mirizzi syndrome (MS) reduce incidence operative complications. Twenty-five cases from January 2005 2010 were retrospectively analyzed. There 11 male patients 14 female patients, ranging ages 26 80 years with a median age 51.3. Preoperative radiological was achieved 10 patients: ultrasonography (n = 5) magnetic resonance cholangiopancreatography 10). others diagnosed intra-operatively. Fifteen had Type I...
As a protective mechanism regulating excessive inflammation, endotoxin tolerance plays vital role in shock. Kupffer cells are players mediating tolerance. Nonetheless, the regulatory is barely known. A nonclassical IKK kinase called TRAF-associated NF-κB activator (TANK)-binding 1 (TBK1) can regulate inflammation. Here, we found that TBK1 required for cells. dominant by negatively induction of p100 processing. Deltex E3 ubiquitin ligase 4 (DTX4), negative regulator TBK1, promote K48-mediated...
AIM:To determine the in vivo effects of phagocytic blockade Kupffer cell (KC) on release proinflammatory cytokines small intestinal lesion and integrity tract by using gadolinium chloride (GdCl 3 ) during early endotoxemia. METHODS:Wistar rats were divided into three groups: Group A, injected with endotoxin (E. coli O111:B 4 , a dose 12 mg.kg - only; B, pretreated intravenously 25 mg GdCl per kg 24 h are given endotoxin; C, sham operation only.All animals sacrificed after injection.In...
Adiponectin (APN) is an adipocyte protein with anti-diabetic properties, which has been recently revealed to have anti-inflammatory activity in organ ischemia- reperfusion injury (IRI). However, little known about its function bile duct IRI after liver transplantation. Therefore, we investigated whether APN affects early warm rat using a autologous transplantation model. In our study, rats were randomly divided into three experimental groups: sham group, group and group. The serum enzyme...
Pellino 1 positively regulates Toll-like receptor 4 signaling by regulating tumor necrosis factor receptor-associated 3 (TRAF3) degradation and is suppressed with the induction of endotoxin tolerance. However, role TRAF3 in tolerance largely unknown. In this study, we found that lipopolysaccharide (LPS) stimulation decreased TARF3 protein expression mouse Kupffer cells (KCs) liver tissues, whereas tolerization abrogated effect. Degradative K48-linked ubiquitination cytoplasmic translocation...
Abstract Objectives The individual differences and pervasive resistance seriously hinder the optimization of irinotecan‐based therapeutic effectiveness. Eukaryotic translation initiation factor 3a (eIF3a) plays a key role in tumour occurrence, prognosis response. This study focused on eIF3a irinotecan‐induced DNA damage Materials Methods cck8 cell viability clone survival analyses were used to test regulatory irinotecan sensitivity HT29 CACO2 lines vitro. was also verified vivo by conducting...
Background: The complex etiology of Ischemia-Reperfusion Injury (IRI) induced by liver transplantation (LT) and the “one-target-focused” method limit development effective therapeutic interventions. We aimed to reveal specific active ingredients mechanisms involved in Chinese herb Scutellaria baicalensis Georgi (SBG) alleviating IRI LT. Methods: potential macromolecular targets SBG were screened through related databases. differentially expressed genes LT obtained from GSE151648....