A5022512665- Chronic Obstructive Pulmonary Disease (COPD) Research
- Pulmonary Hypertension Research and Treatments
- Nitric Oxide and Endothelin Effects
- Ion Channels and Receptors
- Cardiovascular, Neuropeptides, and Oxidative Stress Research
- Ion channel regulation and function
- Respiratory Support and Mechanisms
- Air Quality and Health Impacts
- Listeria monocytogenes in Food Safety
- Respiratory and Cough-Related Research
- Nicotinic Acetylcholine Receptors Study
- Asthma and respiratory diseases
- Microbial Inactivation Methods
- Neuroscience of respiration and sleep
- Biosensors and Analytical Detection
- Ion Transport and Channel Regulation
- Osteoarthritis Treatment and Mechanisms
- Oral microbiology and periodontitis research
- Neonatal Respiratory Health Research
- Identification and Quantification in Food
- Climate Change and Health Impacts
- Metabolomics and Mass Spectrometry Studies
- Alzheimer's disease research and treatments
- Bacterial biofilms and quorum sensing
- Inflammatory mediators and NSAID effects
Sun Yat-sen University
2000-2025
First Affiliated Hospital of Guangzhou Medical University
2014-2024
Guangzhou Medical University
2015-2024
State Key Laboratory of Respiratory Disease
2013-2024
Guangzhou First People's Hospital
2021
Stanford University
2019-2020
Palo Alto University
2020
VA Palo Alto Health Care System
2019-2020
RELX Group (United Kingdom)
2013
RELX Group (United States)
2013
Abstract Due to the lack of effective treatments, osteoarthritis (OA) remains a challenge for clinicians. Quercetin, bioflavonoid, has shown potent anti‐inflammatory effects. However, its effect on preventing OA progression and underlying mechanisms are still unclear. In this study, Sprague–Dawley male rats were divided into five groups: control group, group (monosodium iodoacetate intra‐articular injection), three quercetin‐treated groups. Quercetin‐treated groups treated with intragastric...
Background: Bmpr2 (bone morphogenetic protein receptor 2) mutations are critical risk factors for hereditary pulmonary arterial hypertension (PAH) with approximately 20% of carriers developing disease. There is an unmet medical need to understand how environmental factors, such as inflammation, render mutants susceptible PAH. Overexpressing 5-LO (5-lipoxygenase) provokes lung inflammation and transient PAH in +/ - mice. Accordingly, its metabolite, leukotriene B 4 , candidates the second...
Although the mechanism of osteoarthritis (OA) has been widely studied and use quercetin for OA therapy is well documented, relevant characteristics microbiome metabolism remain unclear. This study reports changes in gut microbiota during a rat model provides an integrative analysis biomechanism. In this study, rats were categorized into 3 different groups: model, treatment, control groups. The was conducted using monoiodoacetate (MIA) injection protocol. group received daily intragastric...
The gut microbiota is widely considered to be involved in several diseases, including atherosclerosis, obesity, chronic obstructive pulmonary disease (COPD) and arterial hypertension (PAH). This study aimed determine if changes the microbiome metabolome play a major role early pathogenesis of PAH. Male Wistar rats were injected with monocrotaline (MCT) (55 mg/kg) at day 1 calcium-sensing receptor (CaSR) antagonist NPS2143 (4.5 mg/kg/d) from days 21. Fecal samples obtained. analyzed by 16S...
<b>Objective</b> To evaluate the effects of a community based integrated intervention for early prevention and management chronic obstructive pulmonary disease (COPD) in China. <b>Design</b> Cluster randomised controlled trial. <b>Setting</b> Eight healthcare units two communities. <b>Participants</b> Of 1062 people aged 40-89, 872 (101 with COPD 771 without COPD) who fulfilled inclusion exclusion criteria were allocated to or usual care programmes. <b>Intervention</b> Participants randomly...
Stromal interaction molecule 1 (STIM1) is a recently discovered membrane-spanning protein thought to sense lumenal Ca 2+ in sarco(endo)plasmic reticulum (SR/ER) and transduce activation of -permeable store-operated channels (SOC) plasmalemma response SR/ER depletion. To evaluate the role STIM1 closely related protein, STIM2, signaling rat distal pulmonary arterial smooth muscle cells (PASMC) during hypoxia, we used fluorescent microscopy -sensitive dye, fura 2, measure basal intracellular...
In pulmonary arterial smooth muscle cells (PASMCs), Ca2+ influx through store-operated channels thought to be composed of canonical transient receptor potential (TRPC) proteins is an important determinant intracellular free calcium concentration ([Ca2+](i)) and vascular tone. Sildenafil, a type V phosphodiesterase inhibitor that increases cellular cGMP, recently identified as promising agent for treatment hypertension. We previously demonstrated chronic hypoxia elevated basal [Ca2+](i) in...
The proliferation of human bronchial smooth muscle cells (HBSMCs) is a key pathophysiological component airway remodeling in chronic obstructive pulmonary disease (COPD) for which pharmacotherapy limited, and only slight improvements survival have been achieved recent decades. Cigarette smoke well-recognized risk factor COPD; however, the pathogenesis cigarette smoke-induced COPD remains incompletely understood. This study aimed to investigate mechanisms by nicotine affects HBSMC...
Background and objective: Serum D-dimer is elevated in respiratory disease. The objective of our study was to investigate the effect on in-hospital 1-year mortality after acute exacerbations chronic obstructive pulmonary disease (AECOPD). Methods: Upon admission, we measured 343 AECOPD patients' serum levels arterial blood gas analysis, recorded their clinical characteristics. level that discriminated survivors non-survivors determined using a receiver operator curve (ROC). risk factors for...
Chronic hypoxia causes remodeling and alters contractile responses in both pulmonary arteries veins. Although have been studied extensively these disorders, the mechanisms by which veins respond to whether contribute chronic hypoxic hypertension remain poorly understood. In arterial smooth muscle, we previously demonstrated that influx of Ca 2+ through store-operated calcium channels (SOCC) thought be composed transient receptor potential (TRP) proteins is likely play an important role...
Background: Epidemiological research and meta-analyses of published data have shown that biomass smoke (BS) is a risk factor for chronic obstructive pulmonary disease (COPD). However, the link between BS COPD lacks experimental confirmation. Objectives: To verify whether can induce pathologic changes systemic oxidative stress, which may be relevant to development emphysema bronchitis in rats. Methods: Rats were exposed BS, cigarette (CS), or clean air (sham) 14 weeks. During exposure, O2,...
Background: Chronic obstructive pulmonary disease (COPD) is characterized by airway remodeling with smooth muscle (ASM) hypertrophy and hyperplasia. Since tobacco use the key risk factor for development of COPD intracellular Ca2+ concentration ([Ca2+]i) plays a major role in both cell proliferation differentiation, we hypothesized that nicotinic acetylcholine receptor (nAChR) activation elevation [Ca2+]i cells (ASMCs). Methods: We examined expression nAChR functions α7-nAChR ASMCs. Results:...
Pathologic lymphatic remodeling in lymphedema evolves during periods of tissue inflammation and hypoxia through poorly defined processes. In human mouse lymphedema, there is a significant increase inducible factor 1 α (HIF-1α), but reduction HIF-2α protein expression endothelial cells (LECs). We questioned whether dysregulated these transcription factors contributes to disease pathogenesis found that LEC-specific deletion Hif2α exacerbated pathology. Even without vascular injury, the loss...
Background Peribronchiolar fibrosis is an important feature of small airway remodeling (SAR) in cigarette smoke-induced COPD. The aim this study was to investigate the role gelatinases (MMP9, MMP2) and epithelial-mesenchymal transition (EMT) SAR related wood smoke (WS) exposure a rat model. Methods Forty-eight female Sprague-Dawley rats were randomly divided into WS group, (CS) group clean air control group. After 4 7 months exposure, lung tissues examined with morphometric measurements,...
The contribution of airway remodeling in chronic obstructive pulmonary disease (COPD) has been well documented, with smooth muscle cell proliferation and migration playing a role the process. Here, we aimed to verify effects fine particulate matter (PM2.5) on human bronchial (HBSMC) explore underlying signaling pathways. HBSMC apoptosis, were measured using flow cytometry, counting transwell assays, respectively. hedgehog pathway was assessed by western blotting measure expression Sonic...
Listeria monocytogenes is a common foodborne pathogen that presents in various food products, posing important threat to public health. The aim of this study was establish rapid and sensitive method with visualization detect L. based on polymerase spiral reaction (PSR). Primers targeting conserved hlyA gene sequence were designed bioinformatics analyses the current available genomes. isothermal amplification PSR can be completed under constant temperature (65ᵒC) within 60 min high...
Background:Hypoxic pulmonary hypertension (HPH) is characterized by vascular remodeling. Intracellular Ca2+concentration ([Ca2+]i) an essential signal for myocyte proliferation. Whether chronic hypoxia (CH) affects the basal [Ca2+]iand Ca2+entry through store- and/or receptor-operated calcium channels (SOCC, ROCC), and whether canonical transient receptor potential (TRPC) proteins are involved in CH-induced Ca2+influx proliferation venous smooth muscle cells (PVSMCs) examined.Methods...