A5036821762- Brain Metastases and Treatment
- Glioma Diagnosis and Treatment
- Cancer Cells and Metastasis
- Single-cell and spatial transcriptomics
- Cancer Genomics and Diagnostics
- Cell Image Analysis Techniques
- Cancer-related molecular mechanisms research
- Long-Term Effects of COVID-19
- Cell Adhesion Molecules Research
- Cancer-related cognitive impairment studies
- Neurogenesis and neuroplasticity mechanisms
- Prostate Cancer Treatment and Research
- Cancer Immunotherapy and Biomarkers
- Intracerebral and Subarachnoid Hemorrhage Research
- Ferroptosis and cancer prognosis
- Cancer-related Molecular Pathways
- Lung Cancer Research Studies
- Advanced Fluorescence Microscopy Techniques
- Barrier Structure and Function Studies
- Macrophage Migration Inhibitory Factor
- RNA modifications and cancer
- Nanoplatforms for cancer theranostics
- Tissue Engineering and Regenerative Medicine
- Renal and related cancers
- Pluripotent Stem Cells Research
Volgograd Institute of Business
2025
VIB-KU Leuven Center for Cancer Biology
2024
Memorial Sloan Kettering Cancer Center
2020-2024
KU Leuven
2024
Kettering University
2023-2024
St. Anne's University Hospital Brno
2015-2022
University Hospital Brno
2015-2022
New York Oncology Hematology
2019-2021
Czech Academy of Sciences, Institute of Biophysics
2015-2020
Masaryk University
2015-2020
Ironing out a survival strategy Metastatic cells display notable ability to adapt to—and even thrive in—harsh microenvironments. One extreme example is leptomeningeal metastases (LM), cancer that enter region of the central nervous system called subarachnoid space, which filled with cerebral spinal fluid (CSF). This anatomic site has limited supply micronutrients such as iron, and it harbors immune cells. Chi et al. used single-cell RNA sequencing study CSF samples from patients LM (see...
A key challenge of analyzing data from high-resolution spatial profiling technologies is to suitably represent the features cellular neighborhoods or niches. Here we introduce covariance environment (COVET), a representation that leverages gene-gene covariate structure across cells in niche capture multivariate nature interactions within it. We define principled optimal transport-based distance metric between COVET niches scales millions cells. Using encode context, developed environmental...
Abstract The identification of fibroblasts and cancer‐associated from human cancer tissue using surface markers is difficult, especially because the used currently are usually not expressed solely by fibroblasts, fibroblast‐specific molecules still under investigation. It was aimed to compare three commercially available antibodies in detection different epitopes (anti‐fibroblast, fibroblast activation protein α, protein). specificity their expression, employing cell lines tumor‐derived...
Leptomeningeal metastasis (LM), or spread of cancer cells into the cerebrospinal fluid (CSF), is characterized by a rapid onset debilitating neurological symptoms and markedly bleak prognosis. The lack reproducible in vitro vivo models has prevented development novel, LM-specific therapies. Although LM allows for longitudinal sampling floating with spinal tap, attempts to culture patient-derived leptomeningeal have not been successful.We, therefore, employ derivatives human breast lung cell...
Leptomeningeal metastasis (LM) is a fatal neurological complication of cancer. Proton craniospinal irradiation (pCSI) has emerged as promising life-prolonging intervention for LM patients, but the response to this treatment varies. Here, we aimed characterize molecular basis pCSI resistance and response. Proteomic analysis CSF collected from patients at baseline (before pCSI), multiple time points post-treatment, identified CXC-motif chemokine, CXCL1, associated with growth. Higher CXCL1...
Medulloblastoma (MB) is a malignant pediatric brain tumor arising in the cerebellum.Although abnormal GABAergic receptor activation has been described MB, studies have not yet elucidated contribution of receptor-independent GABA metabolism to MB pathogenesis.We find primary tumors globally display decreased expression transaminase (ABAT), protein responsible for metabolism, compared with normal cerebellum.However, less aggressive WNT and SHH subtypes express higher ABAT levels metastatic G3...
Abstract Metastasis to the cerebrospinal fluid (CSF)-filled leptomeninges, or leptomeningeal metastasis (LM), represents a fatal complication of cancer. Proteomic and transcriptomic analyses human CSF reveal substantial inflammatory infiltrate in LM. We find solute immune composition setting LM changes dramatically, with notable enrichment IFN-γ signaling. To investigate mechanistic relationships between cell signaling cancer cells within we developed syngeneic lung, breast, melanoma mouse...
Abstract The distribution of fluorescence signals measured with flow cytometry can be influenced by several factors, including qualitative and quantitative properties the used fluorochromes, optical detection system, as well variability within analyzed cell population itself. Most single samples prepared from in vitrocultures or clinical specimens contain a variable cycle component. Cell cycle, together changes size, are two factors that alter functional cells thus affect interpretation...
Abstract Skp2 is a crucial component of SCF E3 ubiquitin ligase and often overexpressed in various types cancer, including prostate cancer (PCa). The epithelial-to-mesenchymal transition (EMT) involved PCa progression. acquisition mesenchymal phenotype that results stem cell (CSC) was described. Therefore, we aimed to investigate the expression localization clinical samples from patients with PCa, association EMT status, role CSC. We found nuclear increased compared those benign hyperplasia,...
The intratumoural heterogeneity, often driven by epithelial-to-mesenchymal transition (EMT), significantly contributes to chemoresistance and disease progression in adenocarcinomas. We introduced a high-throughput screening platform identify surface antigens that associate with epithelial–mesenchymal plasticity well-defined pairs of epithelial cell lines their mesenchymal counterparts. Using multicolour flow cytometry, we then analysed the expression 10 most robustly changed identified...
The cell surface glycoprotein Trop-2 is commonly overexpressed in carcinomas and represents an exceptional antigen for targeted therapy. Here, we provide evidence that expression functionally connected with epithelial phenotype breast prostate lines patient tumor samples. We further show suppressed epigenetically or through the action of epithelial-to-mesenchymal transition transcription factors deregulation linked cancer progression poor prognosis. Moreover, our data suggest...
Deciphering the properties of adult stem cells is crucial for understanding their role in healthy tissue and cancer progression as well. Both have shown association with epithelial-to-mesenchymal transition (EMT) various types. Aiming to investigate epithelial mesenchymal phenotypic traits mouse prostate, we sorted subpopulations basal prostate (mPSCs) assessed expression levels EMT regulators markers custom-designed gene array. The population mPSCs defined by a Lin−/Sca-1+CD49fhi/Trop-2+...
Plasticity of cancer cells, manifested by transitions between epithelial and mesenchymal phenotypes, represents a challenging issue in the treatment neoplasias. Both epithelial-mesenchymal transition (EMT) mesenchymal-epithelial (MET) are implicated processes metastasis formation acquisition stem cell-like properties. Mouse double minute (MDM) 2 MDMX important players progression, as they act regulators p53, but their function EMT may be contradictory. Here, we show that phenotype multiple...
Abstract SARS-CoV-2 infection induces a wide spectrum of neurologic dysfunction. Here we show that particularly vulnerable population with manifestations COVID-19 harbor an influx inflammatory cytokines within the cerebrospinal fluid in absence viral neuro-invasion. The majority these mediators are driven by type 2 interferon and known to induce neuronal injury other disease models. Levels matrix metalloproteinase-10 spinal correlate degree Furthermore, this neuroinflammatory process...
Abstract The epithelial-mesenchymal plasticity, in tight association with stemness, contributes to the mammary gland homeostasis, evolution of early neoplastic lesions and cancer dissemination. Focused on cell surfaceome, we used mouse models pre-neoplastic epithelial stem cells reveal connection between surface markers distinct phenotypes. We mechanistically dissected TGF-β family-driven regulation Sca-1, one most commonly adult markers. further provided evidence that disrupts lineage...