A5091278469- Glioma Diagnosis and Treatment
- Pituitary Gland Disorders and Treatments
- Brain Metastases and Treatment
- Adrenal and Paraganglionic Tumors
- Meningioma and schwannoma management
- Growth Hormone and Insulin-like Growth Factors
- Radiopharmaceutical Chemistry and Applications
- Alzheimer's disease research and treatments
- Mitochondrial Function and Pathology
- Neurofibromatosis and Schwannoma Cases
- Lung Cancer Research Studies
- Neuroscience and Neuropharmacology Research
- Parkinson's Disease Mechanisms and Treatments
- Adrenal Hormones and Disorders
- Estrogen and related hormone effects
- Cardiomyopathy and Myosin Studies
- Single-cell and spatial transcriptomics
- Eosinophilic Esophagitis
- Sarcoma Diagnosis and Treatment
- Cancer Treatment and Pharmacology
- Neuroblastoma Research and Treatments
- Neurogenesis and neuroplasticity mechanisms
- Tumors and Oncological Cases
- Chemical Reactions and Isotopes
- Cerebral Venous Sinus Thrombosis
University of Southern California
2015-2025
Los Angeles Medical Center
2016-2025
LAC+USC Medical Center
2021-2023
University of California, San Diego
2018-2023
University of California, Irvine
2023
Illinois College
2023
Tripler Army Medical Center
2023
Cedars-Sinai Medical Center
2023
Children's Hospital of Los Angeles
2021
Neurological Surgery
2018-2019
Abnormally phosphorylated tau, an indicator of Alzheimer's disease, accumulates in the first decades life locus coeruleus (LC), brain's main noradrenaline supply. However, technical challenges in-vivo assessments have impeded research into role LC disease. We studied participants with or known to be at-risk for mutations genes causing autosomal-dominant disease (ADAD) early onset, providing a unique window pathogenesis largely disentangled from age-related factors. Using high-resolution MRI...
Nuclear receptors form strong dimers that are essential for their function as transcription factors, and it is thought ligand binding can affect dimer stability. In this report, we describe convenient fluorescence resonance energy transfer (FRET)-based methods measuring the thermodynamic kinetic stability of estrogen receptor-alpha ligand-binding domain (ERalpha-LBD). We have developed chemically labeled with a single fluorophore in site-specific manner. These fluorophore-labeled ERs...
Medulloblastoma (MB) is a malignant pediatric brain tumor arising in the cerebellum.Although abnormal GABAergic receptor activation has been described MB, studies have not yet elucidated contribution of receptor-independent GABA metabolism to MB pathogenesis.We find primary tumors globally display decreased expression transaminase (ABAT), protein responsible for metabolism, compared with normal cerebellum.However, less aggressive WNT and SHH subtypes express higher ABAT levels metastatic G3...
Extremely drug-resistant (XDR) Acinetobacter baumannii is a notorious and frequently encountered pathogen demanding novel therapeutic interventions. An initial monoclonal antibody (MAb), C8, raised against A. capsule, proved highly effective treatment minority of clinical isolates. To overcome this limitation, we broadened coverage by developing second for use in combination regimen. We sought to develop an additional anti-A. MAb through hybridoma technology immunizing mice with sublethal...
Abstract Purpose: Limited effective treatments are currently available for central nervous system (CNS) metastasis (CM). This is largely driven by the inability of current therapeutics to penetrate blood brain barrier (BBB) and lack preclinical models testing new therapies. Here we study efficacy AZD1390, a BBB penetrating ataxia-telangiectasia mutated inhibitor, as radiosensitizer breast cancer CM treatment. Experimental Design: Three patient-derived xenograft (PDX) tumors including 2 HER2+...
OBJECTIVE Tumor consistency, or fibrosity, affects the ability to optimally resect meningiomas, especially with recent trends evolving toward minimally invasive approaches. The authors’ team previously validated a practical 5-point scale for intraoperative grading of meningioma consistency. impact consistency on surgical management and outcomes, however, has yet be explored. This study aimed determine associations between presenting symptoms, tumor characteristics, postoperative outcomes....
Abstract Objective Nonfunctioning pituitary adenomas (NFPA) are common, benign lesions of the gland. The endoscopic endonasal approach (EEA) has improved their treatment. Large multi-center data across different healthcare systems on outcomes following EEA resection NFPA limited. We aimed to provide highly generalizable benchmark from an international, review for resection. Methods Institution-level symptoms, tumor and intraoperative characteristics, complications, long-term were obtained...
Balamuthia mandrillaris is a rare, free-living amoeba (FLA) that causes granulomatous amoebic encephalitis, disease with close to 90% mortality. The geographical ranges of many FLA are expanding, potentially increasing human exposure B. mandrillaris. Here, we report case 58-year-old woman progressive neurological symptoms, ultimately diagnosed postmortem encephalitis through plasma metagenomic next-generation sequencing (mNGS) despite negative results on both cerebrospinal fluid (CSF) mNGS...
OBJECTIVE Pituitary adenomas (PAs) are common lesions that often present with endocrinopathy and/or visual symptoms. Classification of PAs has historically been based on functional status and histopathological staining anterior pituitary hormones. In 2017, the WHO revised classification PAs, establishing cell lineages identified by transcription factors (TFs) PIT1, TPIT, SF1. The clinical behavior PA subtypes TF typing, including growth patterns, response to treatment, recurrence rates, is...
INTRODUCTION: Successful surgical treatment of biochemically confirmed Cushing’s Disease (CD) is challenging in patients who have no adenoma visible on preoperative magnetic resonance imaging (MRI). It unclear whether MRI status affects patient outcomes CD. METHODS: In this retrospective study analyzing data from the Registry Adenomas Pituitary and Related Disorders (RAPID), adult with CD were stratified into two groups based presence or absence a imaging. Patients excluded if they had prior...
Synapses are essential for learning and memory, their loss predicts cognitive decline in Alzheimer's disease (AD). Synaptic is associated with excitotoxicity, neuroinflammation, amyloid-β, tau pathology, but the molecular mechanisms remain unclear. There an urgent need to identify new targets modify slow synaptic decline. This study examines if calcium-dependent phospholipase A2 (cPLA2) implicated AD loss. cPLA2 catalyzes membrane phospholipids release arachidonic acid, which can be...
OBJECTIVE Glioblastoma (GBM) is the most prevalent and aggressive of primary brain tumors. There currently no effective treatment for this tumor. The proteasome inhibitor bortezomib a variety tumors, but not GBM. authors' goal was to demonstrate that can be in orthotopic GBM murine model if appropriate method drug delivery used. In study Alzet mini-osmotic pump used bring directly tumor brain, circumventing blood-brain barrier; thus making an METHODS 2 human glioma cell lines, U87 U251, were...
Abstract The Grade of meningioma has significant implications for selecting treatment regimens ranging from observation to surgical resection with adjuvant radiation. For most patients, meningiomas are diagnosed radiologically, and is not determined unless a procedure performed. goal this study train novel auto-classification network determine I II using T1-contrast enhancing (T1-CE) T2-Fluid attenuated inversion recovery (FLAIR) magnetic resonance (MR) images. Ninety-six consecutive naïve...
Missense variants of the valosin-containing protein (VCP) gene cause a progressive, autosomal dominant disease termed VCP multisystem proteinopathy (MSP1). The is constellation clinical features including inclusion body myopathy (IBM), Paget bone (PDB), frontotemporal dementia (FTD), and amyotrophic lateral sclerosis (ALS), typically reported at frequency 90%, 42%, 30%, 9%, respectively. Hispanic population currently underrepresented in previous reports myopathy. We expand our...
Glioblastoma is a heterogeneous tumor for which effective treatment options are limited and often insufficient. Few studies have examined the intratumoral transcriptional proteomic heterogeneity of glioblastoma microenvironment to characterize spatial distribution potential molecular cellular therapeutic immunooncology targets. We applied an integrated multimodal approach comprised NanoString GeoMx Digital Spatial Profiling, single-cell RNA-seq (scRNA-seq), expert neuropathologic assessment...
Better treatments for glioblastoma (GBM) patients, in particular the recurrent setting, are urgently needed. Clinical trials performed Brazil indicated that intranasal delivery of perillyl alcohol (POH) might be effective this patient group. NEO100, a highly purified version POH, was current good manufacturing practice (cGMP) manufactured to evaluate safety and efficacy novel approach Phase I/IIa clinical trial United States.A total 12 patients with GBM were enrolled into I trial. NEO100...
Silent corticotroph adenomas (SCAs) are a distinct subtype of nonfunctioning pituitary (NFAs) that demonstrate positive immunohistochemistry for adrenocorticotropic hormone (ACTH) without causing Cushing's disease. SCAs hypothesized to exhibit more aggressive behavior than standard NFAs. The authors analyzed their institution's surgical experience with in an effort characterize rates invasion, postoperative clinical outcomes, and patterns disease recurrence progression. secondary objectives...
Abstract Background Limited data exist pertaining to outcomes following surgery for recurrent Rathke's cleft cysts (RCC). Objective To determine treatment in patients undergoing reoperation or residual RCCs. Methods A retrospective analysis of 112 consecutive RCC operations 109 between 1995 and 2017 was conducted. Results Eighteen underwent 21 reoperations with a mean follow-up 58 mo. Patient symptoms prior included headaches (14, 66.7%) vision loss (12, 57.1%). Thirteen 18 (72.2%) required...
Abstract Abnormally phosphorylated tau, an indicator of Alzheimer’s disease, accumulates in the first decades life locus coeruleus (LC), brain’s main noradrenaline supply. However, technical challenges reliable in-vivo assessments have impeded research into role LC disease. We studied participants with or known to be at-risk for mutations genes causing autosomal-dominant disease (ADAD) early onset, providing a unique window pathogenesis largely disentangled from age-related factors. Using...
Abstract Objective ACTN2, encoding alpha‐actinin‐2, is essential for cardiac and skeletal muscle sarcomeric function. ACTN2 variants are a known cause of cardiomyopathy without involvement. Recently, specific dominant monoallelic were reported as rare core myopathy variable clinical onset, although the pathomechanism remains to be elucidated. The possibility recessively inherited ACTN2‐myopathy has also been proposed in single series. Methods We provide clinical, imaging, histological...
Site-directed spin labeling (SDSL), the site-specific incorporation of nitroxide spin-labels into a protein, has allowed us to investigate ligand-induced conformational changes in ligand-binding domain human estrogen receptor α (hERα-LBD). EPR (electron paramagnetic resonance) spectroscopy probe attached ER produces different spectra depending upon identity bound ligand; these differences are indicative type and degree motional character spin-label induced by conformations labeled ER. Visual...