A5014839899- Neurogenesis and neuroplasticity mechanisms
- Cell Image Analysis Techniques
- Cholesterol and Lipid Metabolism
- Alzheimer's disease research and treatments
- Neuroinflammation and Neurodegeneration Mechanisms
- Fatty Acid Research and Health
- RNA Research and Splicing
- Mitochondrial Function and Pathology
- Peroxisome Proliferator-Activated Receptors
- Developmental Biology and Gene Regulation
- Diet and metabolism studies
- Nerve injury and regeneration
- Eicosanoids and Hypertension Pharmacology
- Advanced Neuroimaging Techniques and Applications
- Drug Transport and Resistance Mechanisms
- Bioinformatics and Genomic Networks
- Lanthanide and Transition Metal Complexes
- Cellular Mechanics and Interactions
- Pluripotent Stem Cells Research
- Liver Disease Diagnosis and Treatment
- Multiple Sclerosis Research Studies
- Immune cells in cancer
- Machine Learning in Bioinformatics
- Tryptophan and brain disorders
- Advanced Fluorescence Microscopy Techniques
University of Southern California
2021-2025
Southern California University for Professional Studies
2022-2024
Keck Hospital of USC
2022-2024
Istanbul Medipol University
2017-2023
Abdullah Gül University
2022
Yeditepe University
2022
Istanbul University
2022
Akdeniz University
2022
Ordu University
2022
Justus-Liebig-Universität Gießen
2022
Highlights•Reliable method for generation of astrocytes from human iPSCs and ESCs•Generated are functional inflammation-responsive•Generated share properties with primary in vitro•This is a valuable tool disease modeling neuroinflammationSummaryAstrocyte dysfunction neuroinflammation detrimental features multiple pathologies the CNS. Therefore, development methods that produce represents an advance study neurological diseases. Here we report efficient inflammation-responsive astrocyte...
Comparative analyses of neuronal phenotypes in closely related species can shed light on changes occurring during evolution. The study post-mortem brains nonhuman primates (NHPs) has been limited and often does not recapitulate important species-specific developmental hallmarks. We utilize induced pluripotent stem cell (iPSC) technology to investigate the development cortical pyramidal neurons following migration maturation cells grafted developing mouse cortex. Our results show differential...
Apart from its potent antioxidant property, recent studies have revealed that melatonin promotes PI3K/Akt phosphorylation following focal cerebral ischemia (FCI) in mice. However, it is not clear (i) whether increased a concomitant event or directly contributes to melatonin's neuroprotective effect, and (ii) how regulates signaling pathway after FCI. In this study, we showed Akt was intensively phosphorylated at the Thr308 activation loop as compared with Ser473 by Melatonin treatment...
Myelination in the central nervous system is process by which oligodendrocytes form myelin sheaths around axons of neurons. enables neurons to transmit information more quickly and efficiently allows for complex brain functions; yet, remarkably, underlying mechanism myelination occurs still not fully understood. A reliable vitro assay essential dissect oligodendrocyte biology. Hence, we developed a protocol generate myelinating from mouse embryonic stem cells established formation with...
Abstract Background Cellular senescence, a hallmark of aging, has been implicated in Alzheimer’s disease (AD) pathogenesis. Cholesterol accumulation is known to drive cellular senescence; however, its underlying mechanisms are not fully understood. ATP-binding cassette transporter A1 (ABCA1) plays an important role cholesterol homeostasis, and expression trafficking altered APOE4 AD models. However, the ABCA1 senescence associated with remains unclear. Methods We examined association between...
Chronic neuroinflammation plays a key role in the progression of Alzheimer's disease (AD), and cytosolic calcium-dependent phospholipase A2 (cPLA2) enzyme is critical mediator inflammatory lipid signaling pathways. Here we investigate therapeutic potential novel cPLA2 inhibitors modulating AD. By leveraging giga-scale V-SYNTHES 2.0 virtual screening on-demand chemical space conducting two rounds optimization for potency selectivity, have identified BRI-50460, achieving an IC50 0.88 nM...
Background Synapses are essential for learning and memory, their loss predicts cognitive decline in Alzheimer's disease (AD). Synaptic is associated with excitotoxicity, neuroinflammation, amyloid-β, tau pathology, but the molecular mechanisms remain unclear. There an urgent need to identify new targets modify slow synaptic decline. This study examines if calcium-dependent phospholipase A2 (cPLA2) implicated AD loss. cPLA2 catalyzes membrane phospholipids release arachidonic acid, which can...
Abstract Ribbon is a nuclear Broad Tramtrack Bric‐a‐brac (BTB) ‐domain protein required for morphogenesis of the salivary gland and trachea. We recently showed that ribbon mutants exhibit decreased Crumbs Rab11‐coincident apical vesicles increased Moesin activity microvillar structure during tube elongation. To learn how these molecular morphological changes affect dynamics tubulogenesis, we optimized an advanced two‐photon microscope to enable high‐resolution live imaging Live revealed...
Abstract Background Inducing brain ATP-binding cassette 1 (ABCA1) activity in Alzheimer’s disease (AD) mouse models is associated with improvement AD pathology. The purpose of this study was to investigate the effects ABCA1 agonist peptide CS-6253 on amyloid-β peptides (Aβ) and lipoproteins plasma cerebrospinal fluid (CSF) cynomolgus monkeys, a species amyloid lipoprotein metabolism similar humans. Methods injected intravenously into monkeys at various doses three different studies. Plasma...
Apolipoprotein ε allele 4 (APOE4) influences the metabolism of polyunsaturated fatty acids (PUFAs) such as docosahexaenoic acid (DHA). The entorhinal cortex (EC) in brain is affected early Alzheimer's disease and rich DHA. purpose this study to identify effect APOE4 DHA lipid species on EC. Plasma cerebrospinal fluid (CSF) lipidomic measurements were obtained from Brain Delivery Pilot, a randomized clinical trial supplementation (n = 10) versus placebo 12) for six months nondemented older...
Cellular senescence is a hallmark of aging and has been implicated in Alzheimer's disease (AD) pathogenesis. Cholesterol accumulation drives cellular senescence; however, the underlying mechanisms are unclear. ATP-binding cassette transporter A1 (ABCA1) plays an important role cholesterol homeostasis. ABCA1 expression its trafficking afiltered APOE4 AD mouse models. However, whether involved remains unknown.
Myelin disorders burden millions of people around the world, yet existing therapies are inadequate to cure them. Current remedies commonly treat symptoms with minimal no effect on actual cause disorder. The basis and/or mechanism demyelination is not known for many either. In recent years, stem cells variable origin have been used in clinical trials as transplant agents restore defective biochemical process or damaged tissue. We summarize outcomes these disorders. capability reprograming...
Automated screening systems in conjunction with machine learning-based methods are becoming an essential part of the healthcare for assisting disease diagnosis. Moreover, manually annotating data and hand-crafting features training purposes impractical time-consuming. We propose a segmentation classification-based approach assembling automated system analysis calcium imaging. The method was developed verified using effects IgGs (from Amyotrophic Lateral Sclerosis patients) on (Ca2+)...
Docosahexaenoic acid [22:6(n-3), DHA], a polyunsaturated fatty acid, has an important role in regulating neuronal functions and normal brain development. Dysregulated DHA uptake metabolism are found individuals carrying the APOE4 allele, which increases genetic risk for Alzheimer's disease (AD), implicated progression of several neurodegenerative disorders. However, there limited tools to assess kinetics vivo that can be translated humans. Here, we report synthesis ω-radiofluorinated PET...
The Drosophila embryonic salivary gland is an epithelial organ formed by the coordinated invagination and migration of primordial cells. To identify genes that regulate we performed a deficiency screen third chromosome. Here, report on analysis beta 2 tubulin isoform (beta2t) maps at 85D15. We show that, in beta2t mutant embryos, glands did not complete their posterior fusion competent myoblasts longitudinal visceral muscle founder cells between circular mesoderm was delayed. also...