The cellular organization of normal mouse liver was studied using light and electron microscopy quantitative immunocytochemical techniques. general histological the is similar to livers other mammalian species, with a lobular based on distributions portal areas central venules. parenchymal hepatocytes were detected techniques recognize albumin or biotin containing cells. macrophage Kupffer cells identified F4-80 immunocytochemistry, Ito stellate GFAP endothelial labeled CD-34 antibody....

Abstract Background In this study, we assessed the accuracy of genomic prediction for carcass weight (CWT), marbling score (MS), eye muscle area (EMA) and back fat thickness (BFT) in Hanwoo cattle when using best linear unbiased (GBLUP), weighted GBLUP (wGBLUP), a BayesR model. For these models, investigated potential gain from pre-selected single nucleotide polymorphisms (SNPs) genome-wide association study (GWAS) on imputed sequence data gene expression information. We used 13,717 animals...

Whole-genome sequence (WGS) data are increasingly being applied into genomic predictions, offering a higher predictive ability by including causal mutations or single-nucleotide polymorphisms (SNPs) putatively in strong linkage disequilibrium with affecting the trait. This study aimed to improve performance of customized Hanwoo 50 k SNP panel for four carcass traits commercial population adding highly variants from data. A total 16,892 cattle phenotypes (i.e., backfat thickness, weight,...

Data collected from 690 purebred Duroc pigs 2009 to 2012 were used estimate the heritability, and genetic phenotypic correlations between production meat quality traits. Variance components obtained through restricted maximum likelihood procedure using Wombat SAS version 9.0. Animals raised under same management in five different breeding farms. The average daily gain, loin muscle area (LMA), backfat thickness (BF), lean percent (LP) measured as Meat traits included pH, cooking loss,...

Genetic parameters for the reproductive traits of Hanwoo cattle were estimated using data obtained from 15,355 cows in 92 herds across South Korea, which inseminated May 1997 to July 2016. An "average information" restricted maximum likelihood (REML) procedure that fit single-trait and multi-trait animal models was used estimate variance components age at first calving (AFC), interval (CI), days open (DO), gestation length (GL). Results showed low estimates heritability all both models....

Hanwoo is one of the most economically important animal species in Korea due to its significant contribution nutrition. However, current selection index only focuses improve carcass traits Hanwoo. Thus, this study aimed estimate genetic parameters birth weight (BW) and weaning (WW) their phenotypic relationship age at first calving (AFC) gestation length (GL) The for were estimated using data obtained from 52,173 35,800 calves born February 1998 March 2017, respectively. Further, these used...

Hanwoo was originally raised for draft purposes, but the increase in local demand red meat turned that purpose into full-scale meat-type cattle rearing; it is now considered one of most economically important species and a vital food source Koreans. The application genomic selection breeding programs recent years expected to lead higher genetic progress. However, better statistical methods can improve prediction accuracy are required. Hence, this study aimed compare predictive performance...

Immune response of 107 vaccinated Holstein cattle was initially obtained prior to the ELISA test. Five with high and low bovine viral diarrhea virus (BVDV) type I antibody were identified as final experimental animals. Blood samples from these animals then utilized determine significant differentially expressed genes (DEGs) using RNA-seq transcriptome analysis enrichment analysis. Our 261 DEGs in Functional gene ontology (GO) annotations Kyoto Encyclopedia Genes Genomes (KEGG) pathways...

Genome wide association study was conducted to identify and validate candidate genes associated with fatty acid composition of pork.A total 480 purebreed Duroc pigs were genotyped using IlluminaPorcine60k bead chips while the test implemented following genome-wide rapid Mixed Model Regression-Genomic Control (GRAMMAR-GC) approach.A 25, 29, 16 single nucleotide polymorphisms (SNPs) significantly stearic (18:0), oleic (18:1) saturated acids (SFA), respectively. significant variants located on...

Genetic parameters and trends in the average daily gain (ADG), backfat thickness (BF), loin muscle area (LMA), lean percentage (LP), age at 90 kg (D90) were estimated for populations of Landrace Yorkshire pigs. Additionally, correlations between these production traits litter estimated. Litter included total born (TB) number alive (NBA). The data used this study obtained from eight farms during 1999 to 2016. Analyses carried out with a multivariate animal model estimate genetic while...

Hanwoo breed is preferred in South Korea because of the high standards marbling and palatability its meat. Numerous studies have been conducted are ongoing to increase meat production quality this beef population. The aim study was estimate compare genetic parameters for carcass traits using BLUPF90 software. Four models were constructed, single trait pedigree model (STPM), single-trait genomic (STGM), multi-trait (MTPM), (MTGM), pedigree, phenotype, information 7991 cattle. evaluated: Back...

Objective This study was conducted to estimate breed-specific variance components for total number born (TNB), alive (NBA) and mortality rate from birth through weaning including stillbirths (MORT) of three main swine breeds in Korea. In addition, the importance maternal genetic service sire effects estimation models evaluated. Methods Records farrowing traits 6,412 Duroc, 18,020 Landrace, 54,254 Yorkshire sows collected January 2001 September 2016 different farms Korea were used analysis....

The genomic best linear unbiased prediction (GBLUP) method has been widely used in routine evaluation as it assumes a common variance for all single nucleotide polymorphism (SNP). However, this is unlikely the case of traits influenced by major SNP. Hence, present study aimed to improve accuracy GBLUP using weighted (WGBLUP), which gives more weight important markers various carcass Hanwoo cattle, such backfat thickness (BFT), (CWT), eye muscle area (EMA), and marbling score (MS). Linear...

This study aimed to evaluate the accuracy of genomic prediction using single-trait and multi-trait single-step best linear unbiased (ssGBLUP) based on 50 K SNP chip dataset imputed high-density (HD) genotypes for various carcass traits in commercial Hanwoo population such as backfat thickness (BFT), weight (CWT), rib eye area (REA), marbling score (MS). Analyses involved phenotypes from 18,269 animals born between April 2006 June 2017 different herds South Korea well information 16,892...

Genetic parameters and accuracy of genomic prediction for production traits in a Duroc population were estimated. Data on 24 828 purebred pigs born 2000-2016. After quality control procedures, 30 263 single nucleotide polymorphism markers 560 animals remained that used to predict the breeding values individuals. Accuracies predicted average daily gain (ADG), backfat thickness (BF), loin muscle area (LMA), lean percentage (LP) age at 90 kg (D90) between pedigree-based single-step methods...

Abstract Comparison of the multi‐trait animal model and traditional repeatability was carried out using data obtained from 6,424 Landrace 20,835 Yorkshire sows farrowed January 2000 to April 2018 in order estimate genetic parameters for litter traits at different parities. Specifically, records total number born (TNB), alive (NBA), mortality (MORT), stillborn (NSB) mummified pigs (MUM) were used. Although results showed heterogeneity heritability parities, mean estimates found be higher than...

This study was conducted to estimate the genetic parameters of different feeding pattern traits, including average daily feed intake (ADFI), occupation time per day (AOTD), visit (AOTV), rate (ADFR), (AFRV), (AFIV), and number visits (ANVD), their relationship production such as on-test gain (ADG), backfat thickness (BFT), loin muscle area (LMA), lean percentage (LP), efficiency conversion ratio (FCR) five measures residual (RFI1 RFI5), in Duroc pigs (DD). The non-heritable common spatial...

Background: Identification of HER2 protein overexpression and/or amplification the gene are required to qualify breast cancer patients for targeted therapies. In situ hybridization (ISH) assays that identify function as a stand-alone test determination status and rely on manual quantification number genes copies chromosome 17 determine amplification. Methods: To assist pathologists, we have developed uPath Dual ISH Image Analysis Breast (uPath DISH IA) algorithm, an adjunctive aid in...

This study was conducted to estimate the genetic parameters for pork belly traits and muscles in Yorkshire pigs. Each cut into nine parts perpendicular thoracic vertebrae (6th 14th). Traits of including deep pectoral, latissimus dorsi, cutaneous trunci, rectus abdominis, external internal abdominal oblique from 382 purebred pigs were recorded analyzed using SAS Package (9.1) Derivative-free restricted maximum likelihood methods. Heritability estimates ranged 0.27 0.49, while they 0.12 0.66...

Meat quality and carcass characteristics have gained the attention of breeders due to their increasing economic value. Thus, this study investigated genomic prediction efficiencies best linear unbiased (GBLUP) single-step GBLUP (ssGBLUP) for traits associated with meat quality, sensory characteristics, fatty-acid composition. A total 1237 Duroc finishing pigs 654 individuals genotyped using Illumina Porcine SNP 60k marker panel were used in study. Prediction accuracy bias ssGBLUP evaluated a...

The objective of this study was to evaluate the present conventional selection program a swine nucleus farm and compare it with new strategy employing genomic enhanced breeding value (GEBV) as criteria. ZPLAN+ software employed calculate genetic gain, total cost, return profit each strategy. first reflected current program, which progeny test system (CS). second scheme based strictly on information (GS1). third scenario same GS1, but by GEBV further supplemented performance (GS2). last...

Residual feed intake (RFI) gained attention as a potential alternative to the conversion ratio (FCR). Thus, this study aimed estimate genetic parameters for different efficiency (FE) traits (FCR, RFI1 RFI5) and their correlation on-test daily weight gain (ADG), backfat (BFT), loin muscle area (LMA), lean percentage (LP), total (FI) 603 Male Duroc (DD), 295 Landrace (LL), 341 Yorkshire (YY). The common spatial pen effect was also estimated in these traits. Five RFI measures were by regressing...