This study was conducted as part of an epidemiological survey 126 nonsmokers and 178 smokers, showing primary infertility residing around Kolkata region Eastern India. Their lifestyle history including smoking habits along with semen blood were collected. The examined the association cigarette risk infertility, by determining quality, follicle stimulating hormone (FSH), luteinizing (LH), testosterone levels, androgen receptor (AR)-CAG repeat length in a group smokers compared control (non...

Background Aberrant methylation is a known cause of cancer initiation and/or progression. There are scant data on the genome‐wide pattern nonfunctioning pancreatic neuroendocrine tumors (NFPanNETs) and sporadic hereditary NFPanNETs. Methods Thirty‐three tissue samples were analyzed: they included from (n = 9), von Hippel‐Lindau (VHL)‐related 10), multiple endocrine neoplasia type 1 (MEN1)–related NFPanNETs 10) as well normal islet cells 4) for comparison. Genome‐wide CpG profiling was...

With an increase in the abuse of various oral habitual products India over past few decades; incidence potentially malignant conditions as leukoplakia, submucous fibrosis and squamous cell carcinoma (SCC) rates have also increased. No recent study has been conducted reporting scenario cancer Eastern (specifically Kolkata).The present was at Dr. R. Ahmed Dental College, Kolkata during 2010-2011 to find a possible correlation between effects different habits, age, sex types mucosal lesions...

Early detection and quantification of DNA damage in oral premalignancy or malignancy may help management the disease improve survival rates. The comet assay has been successfully utilised to detect premalignant malignancy. However, due invasive nature collecting blood, it be painful for many unwilling patients. This study compares micronucleus (MN) buccal mucosa cells with peripheral blood a subset habit-induced precancer cancer For this, MN exfoliated epithelial was compared leucocytes...

Abstract Background: Arsenic, acting as an endocrine disruptor, causes reproductive malfunctions. Studies have been undertaken to find out whether the co-supplementation of α-tocopherol and ascorbic acid (AT-AA) could reduce arsenic-induced testicular toxicity caused by oxidative stress resulting DNA damage. Methods: Adult male Wistar rats (120±10 g) were given arsenic trioxide [3 mg/kg body weight (b.wt.) per day] for 30 consecutive days supplement group received (400 b.wt. day) (200 day)....

Context: The detrimental effects of arsenic on female reproductive functions may involve overt oxidative stress. Casein and pea [Pisum sativum Linn. (Fabaceae)] proteins have antioxidant properties.Objective: To investigate the role casein- pea-supplemented high-protein diet (HPD) in utero-ovarian protection from toxicity.Materials methods: Adult Wistar rats were orally gavaged with vehicle (Gr-I) or at 3 ppm/rat/d (Gr-II Gr-III) for 30 consecutive days, when they maintained either regular...

We designed a novel method to double-strand Agilent microarrays such that 5mC and 5hmC are incorporated on one DNA strand. Using protein binding we demonstrate the utility of this in exploring how cytosine modification outside CG dinucleotide alter sequence-specific transcription factors.

<b>Context:</b> Single cell gel electrophoresis (SCGE) or comet assay is a rapid and very sensitive fluorescent microscopic method for detecting various forms of DNA damage at individual level.<br> <b>Aims:</b> The aim the present study was to detect extent in oral cancer, submucous fibrosis (OSMF) leukoplakia comparison normal individual.<br>Settings Design: A total 44 consecutive patients with cancer (<i>n</i>=26), (<i>n</i>=12) OSMF (<i>n</i>=6) 10 healthy volunteers epithelia (controls)...

J Oral Pathol Med (2012) 41 : 292–302 Background: Arecanut and smokeless tobacco usage is a major cause for oral submucous fibrosis (OSF) its subsequent development to squamous cell carcinoma in South‐east Asian population. Polymorphisms at N ‐acetyltransferase 2 locus, coding an enzyme catalyzing acetylation of aromatic amines, might DNA adduct formation because improper these polyaromatic hydrocarbons. repair enzymes remove prevent malignancy. Methods: In this hospital‐based study, 100...

Most aggressive thyroid cancers are commonly associated with a BRAFV600E mutation. Preclinical and clinical data in suggest that combined BRAF MEK inhibitor treatment results response, but resistance is common. One mechanism of acquired through persistent activation tyrosine kinase (TK) signaling by alternate pathways. We hypothesized combination therapy multitargeting TK inhibitors (MTKI) might be more effective cancer than single-agent or inhibitors.The drug activity was analyzed to...

Background : The aim of this study is to evaluate the expression human telomerase reverse transcription (hTERT) enzyme in chronic periodontitis (CP) and aggressive (AgP) compared with healthy individuals. Methods: A total 79 individuals consented participate study. sample comprised (n = 30), patients CP AgP 19). Gingival tissue was collected evaluated for hTERT by Western blot immunohistochemical methods. Reverse polymerase chain reaction performed using gingival crevicular fluid (GCF)...

Abstract Background Osteosarcoma (OS), the most common bone tumor in children and adolescents, has high rates of metastasis leading to poor survival. Leucine‐rich repeat containing 15 (LRRC15), a transmembrane protein whose expression is modulated by TGFβ, was recently shown be highly expressed on surface OS cells. Here, we evaluate novel antibody‐drug conjugate (ADC) targeting LRRC15 human cell lines murine xenografts. We compare this new ADC, which conjugated anthracycline derivative...

We report a case of 24-year-old man who presented with complaint reduced mouth opening and burning sensation. On examination, he was clinically diagnosed oral submucous fibrosis (OSF). Following routine biopsy histopathological confirmation OSF, the patient supplemented zinc acetate along vitamin A followed up for 4 months. treatment reported increased Histopathologically re-epithelialisation evident appearance normal rete pegs. The data opening, collagen content epithelial thickness six...

The clinico-histopathological diagnosis of oral verrucous carcinoma (OVC) is often exclusionary and extremely difficult. Distinction from the classical squamous-cell (OSCC) a frequent problem for both clinicians pathologists because extensive nature lesion mimicking an invasive cancer. Immunohistochemistry in this case provides platform studying distinct molecular mechanism by variation expression protein markers. In study, authors have attempted to differentiate OVC (case no 1) OSCC 2)...

Summary. Haemophilia B is an X‐linked recessively inherited bleeding disorder caused by heterogeneous mutations spanning the entire factor IX gene. As spontaneous germ‐line are known to occur mostly at CpG dinucleotides in FIX gene, control of disease would require continuous carrier detection and mutation screening. Identification point mutations, most common type more challenging compared with deletion insertion mutations. We examined haemophilia database identify specific nucleotides gene...

Abstract Background Proper expression and functioning of transcription factors (TFs) are essential for regulation different traits thus could be crucial the development complex diseases. Subjects with Down syndrome (DS) have a higher incidence acute lymphoblastic leukemia (ALL) while solid tumors, like breast cancer (BC) oral (OC), show rare incidences. Triplication human chromosome 21 in DS is associated altered genetic dosage TFs. V-ets erythroblastosis virus E26 oncogene homolog 2 (ETS2)...

Chewing of processed arecanut products with tobacco and betel quid has been attributed to many oral pathological conditions. These are very popular among the youngsters lower economic groups. Genetic predisposition now identified as a major risk factor for increasing susceptibility toward disease these chewers.Our study mainly aims find out LOX (G473A) NQO1 (C609T) polymorphisms present comparison between population (habitually exposed smokeless products) metro-city Kolkata tea-garden...

Melanotic neuroectodermal tumour of infancy (MNTI) is a rare benign neural crest origin that was first described by Krompecher in 1918.1 It predominantly found infancy, with about 92% cases below the age 12 months and 82% 6 months. The predominant site premaxilla though it reported at other sites also including skull, mandible, epididymis brain.2 lesions often have areas bluish discolouration on surface are characterised displacement involved tooth bud local aggressiveness. present report...