A5103150922- RNA Interference and Gene Delivery
- Advanced biosensing and bioanalysis techniques
- Nanoparticle-Based Drug Delivery
- Supramolecular Self-Assembly in Materials
- Chemical Synthesis and Analysis
- Ubiquitin and proteasome pathways
- Histone Deacetylase Inhibitors Research
- Lipid Membrane Structure and Behavior
- Nanopore and Nanochannel Transport Studies
- Click Chemistry and Applications
- Protein Degradation and Inhibitors
- Polydiacetylene-based materials and applications
- RNA Research and Splicing
- Electrospun Nanofibers in Biomedical Applications
- RNA and protein synthesis mechanisms
- Advanced Polymer Synthesis and Characterization
- Intraperitoneal and Appendiceal Malignancies
- Immunotherapy and Immune Responses
- Polymer Surface Interaction Studies
- Inhalation and Respiratory Drug Delivery
- Pharmaceutical Quality and Counterfeiting
- Phagocytosis and Immune Regulation
- Additive Manufacturing and 3D Printing Technologies
- Bacteriophages and microbial interactions
- Ultrasound and Hyperthermia Applications
Centre for Human Drug Research
2021-2025
Leiden University
2021-2025
Ghent University
2017-2023
First Hospital of China Medical University
2022-2023
China Medical University
2022-2023
University at Buffalo, State University of New York
2023
University Medical Center of the Johannes Gutenberg University Mainz
2022
Johannes Gutenberg University Mainz
2022
Sichuan University
2022
Ludwig-Maximilians-Universität München
2022
Messenger RNA (mRNA) is revolutionizing the future of therapeutics in a variety diseases, including neurological disorders. Lipid formulations have shown to be an effective platform technology for mRNA delivery and are basis approved vaccines. In many these lipid formulations, polyethylene glycol (PEG)-functionalized provides steric stabilization thus plays key role improving stability both ex vivo vivo. However, immune responses towards PEGylated lipids may compromise use those some...
Abstract Hepatocellular carcinoma (HCC) is the most frequent type of primary liver cancer and third leading cause for cancer‐related death worldwide. The tumor difficult‐to‐treat due to its inherent resistance chemotherapy. Antistromal therapy a novel therapeutic approach, targeting cancer‐associated fibroblasts (CAF) in microenvironment. CAF‐derived microfibrillar‐associated protein 5 (MFAP‐5) identified as target antistromal HCC with high translational relevance. Biocompatible...
ABSTRACT Soy proteins are gaining more and attention because of its multifunction biodegradability. Silver nanoparticles (AgNPs) introduced into the nanofibers to prevent growth microorganisms over filter media. In present study, multifunctional antimicrobial nanofibrous membranes were prepared by electrospinning soy protein isolate (SPI)/polymide‐6 (PA6)‐silver nitrate system followed ultraviolet reduction. The morphology SPI/PA6 was characterized scanning electron microscopy. Antibacterial...
mRNA based infectious disease vaccines have opened the venue for development of novel nucleic acids-based therapeutics. For all therapeutics dedicated delivery systems are required, where different functionalities and targeting abilities need to be optimized respective applications. One option advanced formulations with tailored properties lipid-polymer hybrid nanoparticles complex nanostructure, which allow combine features several already well described acid systems. Here, we explored...
An injectable thermosensitive hydrogel to promote local and sustained release of small nanosized (10–20 nm) siRNA polyplexes.
Abstract Extracellular and intracellular degradation of nucleic acids remains an issue in non‐viral gene therapy. Understanding biodegradation is critical for the rational design therapeutics order to maintain stability functionality at target site. However, there are only limited methods available that allow determining genetic materials biological environments. In this context, decay kinetics fluorescently labeled plasmid DNA (pDNA) messenger RNA (mRNA) undiluted samples (i.e., human...
Liposomes can efficiently deliver messenger RNA (mRNA) into cells. When mRNA cocktails encoding different proteins are needed, a considerable challenge is to all mRNAs the cytosol of each individual cell. In this work, two methods explored co-deliver varying ratiometric doses red (R) or green (G) fluorescent and it found that packaging same lipoplexes (mingle-lipoplexes) crucial multiple types cells according pre-defined ratio. A mixture containing only one type (single-lipoplexes), however,...
Here we report the design and synthesis of a new class bioorthogonal reagents called hydrazonyl sultones (HS) that serve as stable tautomers highly reactive nitrile imines (NI). Compared to photogenerated NI, HS display broad range aqueous stability tunable reactivity in 1,3-dipolar cycloaddition reaction, depending on substituents, sultone ring structure, solvent conditions. DFT calculations have provided vital insights into → NI tautomerism, including base-mediated anionic tautomerization...
Core cross-linked polymeric micelles (CCPMs) are designed to improve the therapeutic profile of hydrophobic drugs, reduce or completely avoid protein corona formation, and offer prolonged circulation times, a prerequisite for passive active targeting. In this study, we tuned CCPM stability by using bifunctional trifunctional cross-linkers varying cross-linkable polymer block length. For CCPMs, amphiphilic thiol-reactive polypept(o)ides polysarcosine-block-poly(S-ethylsulfonyl-l-cysteine)...
Abstract Improving target versus off‐target ratio in nanomedicine remains a major challenge for increasing drug bioavailability and reducing toxicity. Active targeting using ligands on nanoparticle surfaces is key approach but has limited clinical success. A potential issue the integration of also changes physicochemical properties nanoparticles (passive targeting). Direct studies to understand mechanisms active off‐targeting vivo are by lack suitable tools. Here, biodistribution...
Lipid nanoparticle (LNP) remains the most advanced platform for messenger RNA (mRNA) delivery. To date, mRNA LNPs synthesis is mostly performed by mixing lipids and with microfluidics. In this study, a cost-effective microfluidic setup synthesizing developed. It allows to fine-tune characteristics without compromising LNP properties. compared commercial device (NanoAssemblr) ethanol injection influence of manufacturing conditions on performance investigated. prepared exhibit broader size...
Bacterial resistance to antibiotics threatens the ability treat life-threatening bloodstream infections. Oligonucleotides (ONs) composed of nucleic acid mimics (NAMs) able inhibit essential genes can become an alternative traditional antibiotics, as long they are safely transported in human serum upon intravenous administration and carried across multilayered bacterial envelopes, impermeable ONs. In this study, fusogenic liposomes were considered transport ONs promote their internalization...
Acute myeloid leukemia (AML) is a malignancy with generally high mortality. Although recent advances in AML research have revealed that circRNAs play significant roles progression, our understanding of the leukemogenic mechanism remains very limited. In this study, increased expression hsa_circ_0013880 was observed bone marrow mononuclear cells (BMNCs) patients. Overexpression promotes cell proliferation and migration reduces apoptosis. Mechanistically, could elevate USP32, deubiquitinating...
ABC-type triblock copolymers are a rising platform especially for oligonucleotide delivery as they offer an additional functionality besides the anyhow needed functions of shielding and complexation. The authors present polypept(o)ide-based copolymer synthesized by amine-initiated ring-opening polymerization (ROP) N-carboxyanhydrides (NCAs), comprising block A polysarcosine (pSar), poly(S-ethylsulfonyl-l-cystein) (pCys(SO2 Et)) B bioreversible chemo-selective cross-linking poly(l-lysine)...
Self-assembling peptides are an exemplary class of supramolecular biomaterials broad biomedical utility. Mechanistic studies on the peptide self-assembly demonstrated importance oligomeric intermediates towards properties being formed. In this study, we demonstrate how overall yield assemblies moderated through subtle molecular changes in monomers. This strategy is exemplified with a set surfactant-like (SLPs) different β-sheet propensities and charged residues flanking aggregation domains....
ABC-type triblock copolymers are a rising platform especially for oligonucleotide delivery as they offer an additional functionality besides the anyhow needed functions of shielding and complexation.The authors present polypept(o)ide-based copolymer synthesized by amine-initiated ring-opening polymerization (ROP) N-carboxyanhydrides (NCAs), comprising block A polysarcosine (pSar), poly(S-ethylsulfonyl-l-cystein) (pCys(SO 2 Et)) B bioreversible chemo-selective cross-linking poly(l-lysine)...