A5082533631- Immune Cell Function and Interaction
- Immunotherapy and Immune Responses
- T-cell and B-cell Immunology
- Cancer Immunotherapy and Biomarkers
- X-ray Diffraction in Crystallography
- Crystallization and Solubility Studies
- IL-33, ST2, and ILC Pathways
- CAR-T cell therapy research
- Immunodeficiency and Autoimmune Disorders
- RNA Interference and Gene Delivery
- Immune cells in cancer
- Eosinophilic Esophagitis
- Asthma and respiratory diseases
- Immune Response and Inflammation
- Monoclonal and Polyclonal Antibodies Research
- Peptidase Inhibition and Analysis
- Autoimmune Bullous Skin Diseases
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Skin Protection and Aging
- Diabetes and associated disorders
- Inflammation biomarkers and pathways
- Gut microbiota and health
- Fullerene Chemistry and Applications
- Apelin-related biomedical research
- Cancer, Stress, Anesthesia, and Immune Response
Nippon Medical School
2023-2024
Dokkyo Medical University
2011-2021
Medical & Biological Laboratories (Japan)
2016
Tokyo Medical and Dental University
2006-2012
The University of Tokyo
2000-2011
Chulalongkorn University
2011
Juntendo University
2010
Center of Molecular Immunology (Cuba)
2004-2007
Augusta University
2004
Zero to Three
2004
Abstract The glucocorticoid-induced TNF receptor (GITR), which is a member of the family, expressed preferentially at high levels on CD25+CD4+ regulatory T cells and plays key role in peripheral tolerance that mediated by these cells. GITR also conventional CD4+ CD8+ cells, its expression enhanced rapidly after activation. In this report we show provides potent costimulatory signal to both CD25+ CD25− GITR-mediated stimulation induced anti-GITR mAb DTA-1 or ligand transfectants efficiently...
Peyer's patch (PP) dendritic cells (DCs) have been shown to exhibit a distinct capacity induce cytokine secretion from CD4(+) T compared with DCs in other lymphoid organs such as the spleen (SP). In this study, we investigated whether PP are functionally different SP their ability Ab production B cells. Compared DCs, freshly isolated induced higher levels of IgA naive DC-T cell-B cell coculture system vitro. The by was attenuated neutralization IL-6. addition, induction but not further...
The B7 family member B7-H3 (CD276) plays important roles in immune responses. However, the function of remains controversial. We found that murine specifically bound to Triggering receptor expressed on myeloid cells (TREM)-like transcript 2 (TLT-2, TREML2). TLT-2 was CD8 + T constitutively and activated CD4 cells. Stimulation with transfectants preferentially up-regulated proliferation IFN-γ production Transduction into resulted enhanced IL-2 via interactions B7-H3. Blockade B7-H3:TLT-2...
B7-H1 (CD274) is a T-cell coinhibitory molecule that also often induced on human carcinoma cells, where its expression has been implicated in immune escape. Under inflammatory conditions, inducible normal epithelial cells but little known about involvement conversion of to tumor cells. Here, we show skin-specific accelerates carcinogenesis methylcholantrene (MCA)-induced model squamous cell (SCC). Inflammatory responses by MCA or phorbol ester TPA were clearly inhibited transgenic mice...
IgE autoantibodies are considered to be involved in the pathogenesis of bullous pemphigoid (BP), particularly inflammatory and erythematous phenotypes. To develop reliable enzyme‐linked immunosorbent assays (ELISAs) for detection both BP180 BP230 BP sera, compare ELISA results with clinical features. We used commercially available IgG ELISAs BP230. determine influence excess amounts autoantibodies, all normal sera were tested before after adsorption. The statistically compared various...
Induction of the co-stimulatory molecule CD86 on dendritic cells (DCs) in peripheral tissues is a critical event triggering antigen-specific immune responses. In this study, we propose new small interfering RNA (siRNA)-based therapy using cream-emulsified siRNA, targeting DCs for murine contact hypersensitivity (CH) and atopic dermatitis (AD)-like disease. Topical application siRNA efficiently inhibited CH markedly decreased numbers infiltrating CD86(+) or major histocompatibility complex...
Effective communication is crucial for supporting people with diabetes, yet many medical staff feel unsure about their skills in this area. We evaluated role-playing seminars as a method to improve among staff. From 2008 2024, we conducted 78 2,458 participants, including nurses, dietitians, and pharmacists. Participants engaged realistic simulated scenarios based on common clinical situations of patient-medical interactions, taking roles patients (patient performers), (medical observers....
Eosinophils are multifunctional leukocytes involved in allergic reactions as well adipose tissue regulation. IL‐5 is required for eosinophil survival; however, the vivo mechanisms of regulation not fully understood. A tg mouse model with il5 promoter‐driven EGFP expression was established detecting IL‐5‐producing cells . Il5‐egfp mice expressed high levels gonadal (GAT) cells. + GAT were mainly group 2 innate lymphoid (ILCs). IL‐33 preferentially expanded and eosinophils vivo. ILCs found to...
Background: Immune checkpoint inhibitors (ICIs) can induce immune-related adverse events (irAEs). Liquid biomarkers to predict irAE occurrence are urgently needed. We previously developed an ELISA system specifically detect soluble PD-L1 (sPD-L1) with PD-1-binding capacity (bsPD-L1). Here, we investigated the relationship between sPD-L1 and bsPD-L1 in gastric cancer (GC) non-small cell lung (NSCLC) treated PD-1/PD-L1 blockade their association irAEs. Methods: examined sPD-L1, bsPD-L1, matrix...
The effects of the dietary oligosaccharide raffinose on immune responses, with special reference to its anti-allergic functions, were examined in vivo. First, feeding a diet supplemented 50 g raffinose/kg BALB/c mice significantly (P<0.05) increased interleukin (IL) 12 secretion from isolated Peyer's patch (PP) cells vitro compared control diet. When PP used as antigen-presenting (APC) for CD4+ T-splenocytes ovalbumin (OVA)-specific T-cell receptor transgenic (Tg) presence OVA antigen,...
Summary B7‐H3 is a B7‐family co‐stimulatory molecule and broadly expressed on various tissues immune cells. Transduction of into some tumours enhances anti‐tumour responses. We have recently found that triggering receptor myeloid cell‐like transcript 2 (TLT‐2) for B7‐H3. Here, we examined the roles tumour‐associated involvement TLT‐2 in immunity. Ovalbumin (OVA) 257–264 ‐specific OT‐I CD8 + T cells exhibited higher cytotoxicity against B7‐H3‐transduced OVA‐expressing tumour (B7‐H3/E.G7)...
Abstract Keratinocytes (KCs) may play important roles for maintenance of peripheral tolerance in the upper layers skin. Coinhibitory signals mediated via programmed death (PD)-1 and its ligand B7-H1 (PD-L1/CD274) are crucial downregulation T cell immune responses tolerance. In this study, to investigate role KC-expressed regulation responses, we generated transgenic (tg) mice overexpressing under control keratin 14 (K14) promoter (K14-B7-H1 tg). K14-B7-H1 tg displayed impaired contact...
Ligands of the B7 family provide both positive and negative costimulatory signals to CD28 receptors on T lymphocytes, balance which determines immune response. B7-H3 is a member whose function in T-cell activation has been subject some controversy: autoimmunity tumor immunity, it described as coinhibitory, while transplantation, signaling thought contribute graft rejection. However, we now demonstrate results contrary. Signaling through putative receptor prolonged allograft survival fully...
Glucocorticoid-induced tumour necrosis factor receptor-related receptor (GITR) costimulates functions of both effector and regulatory T cells. The administration agonistic anti-GITR monoclonal antibodies efficiently enhances various T-cell-mediated immune responses; however, it is unknown to what extent the ligand GITR (GITRL) contributes T-cell responses. We investigated involvement endogenously expressed GITRL on dendritic cells ectopically tumours in immunity. Expression secondary...
The tumor microenvironment (TME) impacts the therapeutic efficacy of immune checkpoint inhibitors (ICIs). No liquid biomarkers are available to evaluate TME heterogeneity. Here, we investigated clinical significance PD-1-binding soluble PD-L1 (bsPD-L1) in gastric cancer (GC) patients and non-small cell lung (NSCLC) treated with PD-1/PD-L1 blockade.
Glucocorticoid-induced TNFR-related protein (GITR) has various roles in the activation of T cells and inflammation. In this study, we investigated GITR-GITR ligand (GITRL) pathway contact hypersensitivity (CH). Treatment with anti-GITRL mAb at sensitization inhibited CH responses. Depletion studies using an anti-CD25 or anti-PDCA-1 revealed that regulatory plasmacytoid dendritic (DCs), known to express high levels GITR GITRL, respectively, were not apparently involved GITRL-mediated...
// Masahiko Itoh 1 , Derek C. Radisky 2 Masaaki Hashiguchi 3 and Hiroyuki Sugimoto Department of Biochemistry, School Medicine, Dokkyo Medical University, Mibu, Tochigi, Japan Cancer Biology, Mayo Clinic, Jacksonville, Florida, USA Immunology, Correspondence to: Itoh, email: mitoh@dokkyomed.ac.jp Keywords: breast cancer, epithelial-mesenchymal transition, invasion, alternative splicing, RhoGEF Received: July 18, 2017 Accepted: August 21, Published: September ABSTRACT Breast cancer invasion...