Emily Conibear
A5027768203- SARS-CoV-2 and COVID-19 Research
- SARS-CoV-2 detection and testing
- COVID-19 epidemiological studies
- COVID-19 Clinical Research Studies
- Respiratory viral infections research
- interferon and immune responses
- vaccines and immunoinformatics approaches
- Immune responses and vaccinations
- Viral gastroenteritis research and epidemiology
- Zebrafish Biomedical Research Applications
- Retinal Development and Disorders
- Immunotherapy and Immune Responses
- Long-Term Effects of COVID-19
- Advanced Fluorescence Microscopy Techniques
Imperial College London
2020-2025
NIHR Imperial Biomedical Research Centre
2021-2025
Cross-reactive immune responses to SARS-CoV-2 have been observed in pre-pandemic cohorts and proposed contribute host protection. Here we assess 52 COVID-19 household contacts capture at the earliest timepoints after exposure. Using a dual cytokine FLISpot assay on peripheral blood mononuclear cells, enumerate frequency of T cells specific for spike, nucleocapsid, membrane, envelope ORF1 epitopes that cross-react with human endemic coronaviruses. We observe higher frequencies cross-reactive...
Knowledge of the window SARS-CoV-2 infectiousness is crucial in developing policies to curb transmission. Mathematical modelling based on scarce empirical evidence and key assumptions has driven isolation testing policy, but real-world data are needed. We aimed characterise across full course infection a community setting.The Assessment Transmission Contagiousness COVID-19 Contacts (ATACCC) study was UK prospective, longitudinal, cohort contacts newly diagnosed, PCR-confirmed index cases....
Background Infectiousness of respiratory viral infections is quantified as plaque forming units (PFU), requiring resource-intensive culture that not routinely performed. We hypothesised RNA load (VL) decline time (e-folding time) in people might serve an alternative marker infectiousness. Aim This study’s objective was to evaluate the association VL with and PFU area under curve (AUC) transmission risk for SARS-CoV-2 influenza A virus. Methods In virus community cohorts, by reverse...
Abstract Blood transcriptional biomarkers of acute viral infections typically reflect type 1 interferon (IFN) signalling, but it is not known whether there are biological differences in their regulation that can be leveraged for distinct translational applications. We use high frequency sampling the SARS-CoV-2 human challenge model to show induction IFN-stimulated gene (ISG) expression with different temporal and cellular profiles. MX1 correlates a rapid transient wave ISG across all cell...
Summary Evaluation of host-response blood transcriptional signatures viral infection have so far failed to test whether these biomarkers reflect different biological processes that may be leveraged for distinct translational applications. We addressed this question in the SARS-CoV-2 human challenge model. found differential time profiles interferon (IFN) stimulated responses represented by measurement single genes. MX1 transcripts correlated with a rapid and transient wave type 1 IFN genes...
Background: The SARS-CoV-2 Delta variant is highly transmissible and spreading globally but a detailed understanding of community transmission risks in vaccinated populations lacking.Methods: Between September 2020 August 2021, we recruited 510 contacts 422 UK COVID-19 cases to cohort study. A total 7194 upper respiratory tract (URT) samples were tested from sequential daily sampling participants for up 20 days. We analysed risk by vaccination status 139 exposed the variant. compared viral...
The success of case isolation and contact tracing for the control severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) transmission depends on accuracy speed identification. We assessed whether inclusion additional symptoms alongside three canonical (CS),
The relationship between gastrointestinal tract infection, the host immune response, and clinical outcome of disease is not well understood in COVID-19. We sought to understand effect intestinal responses SARS-CoV-2 on patient outcomes including magnitude systemic antibody induction. Combining two prospective cohort studies, International Severe Acute Respiratory emerging Infections Consortium Comprehensive Clinical Characterisations Collaboration (ISARIC4C) Integrated Network for...
<ns5:p>The availability of transparent zebrafish mutants (either<ns5:italic>TraNac</ns5:italic>:<ns5:italic>tra<ns5:sup>b6/b6</ns5:sup>; nac<ns5:sup>w2/w2</ns5:sup></ns5:italic>or<ns5:italic>casper: roy<ns5:sup>a9/a9</ns5:sup>; nac<ns5:sup>w2/w2</ns5:sup></ns5:italic>) for live imaging studies together with the ease generating transgenic lines are two strengths model organism. The fact that...
Abstract SARS-CoV-2 immune-escape variants have only been observed to arise in immunosuppressed COVID-19 cases, during prolonged viral shedding. Through daily longitudinal RT-qPCR, quantitative culture and sequencing, we observe for the first time evolution of transmissible harbouring mutations consistent with mild community cases within 2 weeks infection.