A5025224948- Monoclonal and Polyclonal Antibodies Research
- Cell Adhesion Molecules Research
- Rheumatoid Arthritis Research and Therapies
- Immune Cell Function and Interaction
- Protease and Inhibitor Mechanisms
- Phagocytosis and Immune Regulation
- Immune cells in cancer
- S100 Proteins and Annexins
- Toxin Mechanisms and Immunotoxins
- HER2/EGFR in Cancer Research
Karolinska Institutet
2017-2023
Science for Life Laboratory
2020
Southern Medical University
2017-2018
Abstract Although elevated levels of anti-citrullinated protein antibodies (ACPAs) are a hallmark rheumatoid arthritis (RA), the in vivo functions these remain unclear. Here, we have expressed monoclonal ACPAs derived from patients with RA, and analyzed their mice, as well specificities. None showed arthritogenicity nor induced pain-associated behavior mice. However, one antibodies, clone E4, protected mice antibody-induced arthritis. E4 binding pattern restricted to skin, macrophages...
Today, it is known that autoimmune diseases start a long time before clinical symptoms appear. Anti-citrullinated protein antibodies (ACPAs) appear many years the onset of rheumatoid arthritis (RA). However, still unclear if and how ACPAs are arthritogenic. To better understand molecular basis pathogenicity ACPAs, we investigated autoantibodies reactive against C1 epitope collagen type II (CII) its citrullinated variants. We found these commonly occurring in RA. A mAb (ACC1) was to...
Tumor-associated macrophages (TAMs) continuously fine tune their immune modulatory properties, but how gene expression programs coordinate this cell plasticity is largely unknown. Selective mRNA translation, controlled by MNK1/MNK2 and mTOR pathways impinging on eIF4E, facilitates reshaping of proteomes without changes in abundance corresponding mRNAs. Using polysome profiling developed for small samples we show that, during tumor growth, TAMs predominately modulated via mRNA-selective...
Objective Cartilage oligomeric matrix protein (COMP) is an autoantigen in rheumatoid arthritis (RA) and experimental models of arthritis. This study was undertaken to investigate the structure, function, relevance anti‐COMP antibodies. Methods We investigated pathogenicity monoclonal antibodies mice using passive transfer experiments, we explored interaction with cartilage immunohistochemical staining. The antibody 15A11 complex its specific COMP epitope P6 determined by x‐ray...
Abstract Rheumatoid arthritis (RA) involves several classes of pathogenic autoantibodies, some which react with type-II collagen (COL2) in articular cartilage. We previously described a subset COL2 antibodies targeting the F4 epitope (ERGLKGHRGFT) that could be regulatory. Here, using phage display, we developed recombinant against this and examined underlying mechanism action. One these antibodies, R69-4, protected cartilage antibody- collagen-induced mice, but not autoimmune disease models...
Abstract Background Antibodies binding to cartilage proteins are present in the blood and synovial fluid of early rheumatoid arthritis patients. In order develop animal models mimicking human disease, we have characterized arthritogenic capacity monoclonal antibodies directed towards different joint cartilage. Methods Purified specific unmodified or citrullinated collagen type II (CII), XI (CXI), oligomeric matrix protein (COMP) were produced as culture supernatant, affinity purified, pooled...
Collagen XI (CXI) is a heterotrimeric molecule with triple helical structure in which the α3(XI) chain identical to α1(II) of collagen II (CII), but extensive posttranslational modifications. CXI molecules are intermingled cartilage fibers, mainly composed CII. One alpha chains shared CII and contains immunodominant T cell epitope, it unclear whether there B epitopes as antibodies tend recognize structures.Mice expressing susceptible immune response gene Aq were immunized or CXI. Serum...