A5063037201- SARS-CoV-2 and COVID-19 Research
- Vector-Borne Animal Diseases
- Wastewater Treatment and Nitrogen Removal
- Plant Virus Research Studies
- Biosensors and Analytical Detection
- Viral Infections and Vectors
- COVID-19 Clinical Research Studies
- SARS-CoV-2 detection and testing
- Constructed Wetlands for Wastewater Treatment
- RNA Interference and Gene Delivery
- Water Quality Monitoring Technologies
- Antibiotic Resistance in Bacteria
- interferon and immune responses
- Diverse Scientific Research Studies
- Bacterial Genetics and Biotechnology
- Ammonia Synthesis and Nitrogen Reduction
- Infection Control and Ventilation
- Pharmaceutical and Antibiotic Environmental Impacts
- COVID-19 and healthcare impacts
- Protein Structure and Dynamics
- Antibiotics Pharmacokinetics and Efficacy
- Biotin and Related Studies
- MicroRNA in disease regulation
- Water Treatment and Disinfection
- HIV/AIDS drug development and treatment
Rice University
2020-2025
Maldives National University
2022-2025
John Wiley & Sons (United States)
2021
Hudson Institute
2021
George Washington University
2020
District of Columbia Water and Sewer Authority
2020
Universiti Teknologi MARA
2015
Goethe University Frankfurt
2009-2015
Indian Institute of Science Bangalore
2005-2013
Jawaharlal Nehru Centre for Advanced Scientific Research
2005
The signal peptide plays a key role in targeting and membrane insertion of secretory proteins both prokaryotes eukaryotes. In E. coli, recombinant can be targeted to the periplasmic space by fusing naturally occurring sequences their N-terminus. model protein thioredoxin was fused at its N-terminus with malE pelB sequences. While WT fusion are soluble when expressed, inclusion bodies refolded vitro yield monomeric product identical secondary structure thioredoxin. purified were studied...
Wet weather events, such as hurricanes and tropical storms, are on the rise globally due to climate change. Activated sludge systems vulnerable wet weather, hydraulic overloading can cause a washout of biomass. Biofilm-based treatment technologies, moving bed biofilm reactors (MBBR), improve resiliency by preventing biomass protecting slow-growing nitrifiers. In this study, we investigated resilience system challenged events examined impact different disturbances system's microbial...
Abstract Membrane‐aerated biofilm reactors (MABRs) are being increasingly implemented at full‐scale for domestic wastewater treatment and effective control is critical to their performance. This study investigated the impact of three scouring strategies on nitrogen removal performance a pilot‐scale MABR operated in Houston, TX: (1) regular air scouring, (2) high intensity (3) liquid flow scouring. Normal regimes scour (10× baseline flow) were each tested sequentially. High NH 4 + ‐N...
Abstract Primary sludge fermentate, a concentrated hydrolyzed wastewater carbon, was evaluated for use as an alternative carbon source mainstream partial denitrification–anammox (PdNA) in suspended growth activated process terms of denitrification (PdN) efficiency, PdNA nitrogen removal contributions, and final effluent quality. Fermenter operation at 2‐day retention time (SRT) resulted the maximum achievable yield 0.14 ± 0.05 g sCOD/g VSS without release excessive ammonia phosphorus to...
The recently emerged novel coronavirus, "severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2)," caused a highly contagious disease called coronavirus 2019 (COVID-19). It has severely damaged the world's most developed countries and turned into major threat for low- middle-income countries. Since its emergence in late 2019, medical interventions have been substantial, relied on public health measures collectively known as nonpharmaceutical (NPIs). We aimed to centralize accumulative...
Protein B2 from Nodamura virus (NMV B2), a member of the Nodavirus family, acts as suppressor RNA interference (RNAi). The N-terminal domain NMV B2, consisting residues 1-79, recognizes double-stranded (dsRNA). 2.5 A crystal structure RNA-binding shows dimeric, helical bundle structure. conserved set compared with flock house despite limited sequence identity. packing places along one face symmetry-related molecules, suggesting potential platform for recognition dsRNA.
Mammalian homologs of lin28: Lin28 and Lin28B block the post‐transcriptional processing let‐7 family miRNAs. We report that in vitro terminal stem‐loop region let‐7g miRNA precursor (pre‐let‐7g) required to bind is restricted 24 nucleotides (nt) including 3′ GGAG motif. Additionally, full length for efficient binding pre‐let‐7g stoichiometry complex 1:1. Molecular dynamics (MD) simulations reveal interactions motif stem cold shock domain (CSD) zinc knuckle (ZKD) Lin28B, respectively.
Most HIV-1 broadly neutralizing antibodies are directed against the gp120 subunit of env surface protein. Native consists a trimer gp120-gp41 heterodimers, and in contrast to monomeric gp120, preferentially binds CD4 binding site (CD4bs)-directed over non-neutralizing ones. Some cryo-electron tomography studies have suggested that V1V2 loop regions located close interface. We therefore designed cyclically permuted variants with without h-CMP SUMO2a trimerization domains inserted into loop....
Disulfides cross-link residues in a protein that are separated primary sequence and stabilize the through entropic destabilization of unfolded state. While removal naturally occurring disulfides leads to destabilization, introduction engineered does not always lead significant stabilization protein. We have analyzed span adjacent antiparallel strands β sheets (cross-strand disulfides). Cross-strand recently been implicated as redox-based conformational switches proteins such gp120 CD4. The...
To evaluate the threat of environmental dissemination antibiotic resistance associated with wastewater treatment plants (WWTPs), removal efficiency genes (ARGs) during needs to be assessed. The sample collection strategy is one factor that often overlooked in study design and most studies on ARGs perform grab sampling. Here, we hypothesized sampling (i.e., composite sampling) influences observed ARG concentrations calculated rates across WWTPs. We compared based two different methods for...
Abstract As the COVID-19 pandemic continues to affect communities across globe, need contain spread of outbreaks is paramount importance. Wastewater monitoring SARS-CoV-2 virus, causative agent responsible for COVID-19, has emerged as a promising tool health officials anticipate outbreaks. interest in wastewater grow and municipalities begin implement this approach, there further identify evaluate methods used concentrate virus RNA from samples. Here we recovery, cost, throughput five...
Nodamura virus (NoV) B2, a suppressor of RNA interference, binds double stranded RNAs (dsRNAs) and small interfering (siRNAs) corresponding to Dicer substrates products. Here, we report that the amino terminal domain NoV B2 (NoV 79) specifically siRNAs but not dsRNAs. 79 oligomerizes on binding 27 nucleotide siRNA. Mutation residues phenylalanine49 alanine60 cysteine methionine, respectively enhances affinity 79. Circular dichroism spectra demonstrated wild type mutant have similar secondary...
In 2003, severe acute respiratory syndrome (SARS) caused by SARS coronavirus (CoV) affected 26 countries with 8000 cases.The disease 2019 (COVID-19) SARS-CoV-2 was first reported in Wuhan, China December 2019.It rapidly evolved into a pandemic resulting an unprecedented health crisis.Antiviral drugs and vaccines against related coronaviruses are crucial to prevent any future epidemics pandemics.RNA interference (RNAi), RNA guided post transcriptional gene silencing mechanism, plays important...
In Bangladesh, surface water (e.g., pond/river water) and rainwater are important sources of potable water, particularly in areas suffering from arsenic contamination groundwater high salinity.However, often suffers microbial needs disinfection for use.Disinfection is a challenge small supply systems, as family or communities do not have necessary facilities to disinfect properly.Many commercially available household-level treatment systems effective removing pathogens.This study presents an...