A5011917656- Immunotherapy and Immune Responses
- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- RNA Interference and Gene Delivery
- Pituitary Gland Disorders and Treatments
- Cytomegalovirus and herpesvirus research
- Neuroscience and Neuropharmacology Research
- Microscopic Colitis
- Ion channel regulation and function
- Ion Transport and Channel Regulation
- Renal Transplantation Outcomes and Treatments
- Inflammatory Bowel Disease
- Cancer Research and Treatments
- Colorectal Cancer Screening and Detection
Istituti di Ricovero e Cura a Carattere Scientifico
2020-2023
Istituto di Ricovero e Cura a Carattere Scientifico San Raffaele
2021-2023
Vita-Salute San Raffaele University
2007-2023
The San Raffaele Telethon Institute for Gene Therapy
2020-2023
Università Cattolica del Sacro Cuore
2020
The prominent role of dendritic cells (DC) in promoting tolerance and the development methods to generate clinical grade products allowed application tolerogenic DC (tolDC)-based therapies for controlling unwanted immune responses. We established an efficient method human DC, producing supra-physiological levels IL-10, by genetically engineering monocyte-derived with a bidirectional Lentiviral Vector (bdLV) encoding IL-10 marker gene. IL−10 are mature modulate T cell responses, promote...
Tolerogenic dendritic cells (tolDC) play a central role in regulating immune homeostasis and promoting peripheral tolerance. These features render tolDC promising tool for cell-based approaches aimed at inducing tolerance T-cell mediated diseases allogeneic transplantation. We developed protocol to generate genetically engineered human overexpressing IL-10 (DCIL-10) by means of bidirectional lentiviral vector (LV) encoding IL-10. DCIL-10 promote allo-specific T regulatory type 1 (Tr1) cells,...