A5039725947- Prostate Cancer Treatment and Research
- Bladder and Urothelial Cancer Treatments
- Prostate Cancer Diagnosis and Treatment
- Renal cell carcinoma treatment
- Epigenetics and DNA Methylation
- Advanced Radiotherapy Techniques
- RNA modifications and cancer
- Urinary and Genital Oncology Studies
- Cancer Immunotherapy and Biomarkers
- Immune cells in cancer
- Renal and related cancers
- Cancer, Hypoxia, and Metabolism
- Urinary Bladder and Prostate Research
- Nutrition and Health in Aging
- Urologic and reproductive health conditions
- Photodynamic Therapy Research Studies
- Cancer, Lipids, and Metabolism
- Tissue Engineering and Regenerative Medicine
- Urological Disorders and Treatments
- Ferroptosis and cancer prognosis
- Inflammatory mediators and NSAID effects
- Nanoplatforms for cancer theranostics
- Cancer, Stress, Anesthesia, and Immune Response
- Cancer Genomics and Diagnostics
- Hepatocellular Carcinoma Treatment and Prognosis
Nara Medical University Hospital
2014-2025
Nara Medical University
2015-2024
King's College Hospital NHS Foundation Trust
2024
Shirasagi Hospital
2023-2024
International University of Health and Welfare
2024
John Wiley & Sons (United States)
2019
Hudson Institute
2019
National Archives and Records Administration
2013
Kyushu University Hospital
2006-2013
Kyushu University Beppu Hospital
2010-2013
Tumor-associated macrophages (TAMs) and cancer-associated fibroblasts (CAFs) are reported to be associated with poor prognosis, depending on their pro-tumoral roles. Current knowledge of TAMs CAFs in the tumor microenvironment urothelial cancer bladder (UCB) is limited. Therefore, we investigated paracrine effect induced by human UCB. For this, first carried out immunohistochemical analysis for CXCL1, CD204 (TAM marker), αSMA (CAF E-cadherin, MMP2 using 155 UBC tissue samples. Next,...
Abstract We recently identified a novel human AlkB homologue, ALKBH8, which is expressed in various types of cancers including urothelial carcinomas. In examining the role and function ALKBH8 bladder cancer development vitro, we found that silencing through small interfering RNA transfection reduced reactive oxygen species (ROS) production via down-regulation NAD(P)H oxidase-1 (NOX-1) induced apoptosis subsequent activation c-jun NH2-terminal kinase (JNK) p38. However, also JNK p38 resulted...
Current knowledge of the molecular mechanism driving tumor budding is limited. Here, we focused on elucidating detailed underlying in urothelial cancer bladder. Invasive was pathologically classified into three groups as follows: nodular, trabecular, and infiltrative (tumor budding). Pathohistological analysis orthotopic model revealed that human cell lines MGH-U3, UM-UC-14, UM-UC-3 displayed typical patterns, respectively. Based results comprehensive gene expression using microarray (25 K...
The clinical significance of regulatory T cells (Treg) and tumor-associated macrophages (TAM) in the tumor microenvironment human bladder cancer remains unclear. aim this study is to explore their relevance oncological features non-muscle invasive (NMIBC). We carried out immunohistochemical analysis forkhead box P3 (FOXP3, Treg maker), CD204 (TAM marker), interleukin-6 (IL6) using surgical specimens obtained from 154 NMIBC patients. TAM counts surrounding lesion IL6-positive cell were...
Abstract Background- Data exist that demonstrate isoflavones' potent antiproliferative effects on prostate cancer cells. We evaluated the efficacy of isoflavone in patients with PSA recurrent after prior therapy. postulated therapy would slow rate rise serum PSA. Methods- Twenty rising local were enrolled this open-labeled, Phase II, nonrandomized trial (Trial registration # NCT00596895). Patients treated soy milk containing 47 mg isoflavonoid per 8 oz serving three times day for 12 months....
Memory T cells are well known to have a critical role for host defense in humans. However, their actual human cancer remains largely unknown. In this study, we tried reveal the clinical importance of tumour-infiltrating CD45RO+ memory renal cell carcinoma (RCC). We analysed 105 patients with RCC, who received radical or partial nephrectomy. Those were 65 TNM stage I, 7 II, 15 III, and 18 IV, respectively. CD45RO expression was evaluated by immunohistochemistry. CD4 CD8 expressions also...
Although the significance of preoperative nutritional status has been investigated, there is no report regarding relationship their postoperative changes on outcomes in patients who underwent radical cystectomy for bladder cancer. Here, we clinical impact change, from baseline, and volume abdominal skeletal muscle mass adipose tissue after cystetomy. A retrospective analysis 89 with cancer, curative cystectomy, was conducted to assess time course body composition at 1, 3, 6, 12, 24 months,...
Abstract Purpose: The role and function of a novel human AlkB homologue, ALKBH3, in urothelial carcinoma development were examined. Experimental design: Biologic roles ALKBH3 examined by gene silencing analysis using vitro vivo siRNA transfection. Immunohistochemical analyses the related molecules bladder cancer samples conducted to estimate association with clinicopathologic or prognostic parameters. Results: knockdown induced cell cycle arrest at G1 phase through downregulation NAD(P)H...
The present study evaluated the clinical relevance of an integrative preoperative assessment inflammation-, nutrition-, and muscle-based markers for patients with muscle-invasive bladder cancer (MIBC) undergoing curative radical cystectomy (RC).The analysis enrolled 117 variables included age, body mass index (BMI), neutrophil-to-lymphocyte ratio, monocyte-to-lymphocyte platelet-to-lymphocyte modified Glasgow Prognostic Score (mGPS), prognostic nutritional (PNI), Controlling Nutritional...
The evidence of association between sexually transmitted infection and prostatic inflammation in human prostate cancer (PCa) is limited. Here, we sought to examine the potential with inflammatory environment carcinogenesis. We screened surgical biopsy specimens from 45 patients PCa against a panel infection-related organisms using polymerase chain reaction examined severity intraprostatic by pathologic examination. Among tested organisms, rate Mycoplasma genitalium (Mg) was significantly...
Sarcopenia is a muscle loss syndrome known as risk factor of various carcinomas. The impact sarcopenia and sarcopenia-related inflammatory/nutritional markers in metastatic urothelial carcinoma (mUC) treated with pembrolizumab was unknown, so this retrospective study 27 patients performed. Psoas mass index (PMI) calculated by bilateral psoas major area at the L3 computed tomography. cut-off PMI value for defined ≤6.36 cm2/m2 men ≤3.92 women. Neutrophil-to-lymphocyte ratio (NLR) ≥ 4.0...
Abstract Background Reactive oxygen species (ROS) production via NADPH oxidase (NOX) contributes to various types of cancer progression. In the present research, we examined pathobiological role (NOX)4-mediated generation reactive in urothelial carcinoma (UC) urinary bladder, and demonstrated utility ROS labeling urine cytology. Methods NOX4 gene was silenced vivo vitro by siRNA transfection with or without atlocollagen. Cell cycle measurement were analyzed flowcytometry. Orthotopic...
Primary androgen deprivation therapy (PADT) is the most effective systemic for patients with metastatic prostate cancer. Nevertheless, once PSA progression develops, prognosis serious and mortal. We sought to identify factors that predicted in a series of Two-hundred eighty-six cancer who received PADT from 1998 2005 Nara Uro-Oncology Research Group were enrolled. The log-rank test Cox's proportional hazards model used determine predictive prognosis; rate castration-resistant (CRPC) overall...
Abstract Background COX-2 inhibitors have an antitumor potential and been verified by many researchers. Treatment of cancer cells with external stressors such as irradiation can stimulate the over-expression possibly confer radiation resistance. In this study, we tested if topical diclofenac, which inhibits both COX-1 COX-2, administration rendered prostate tumor sensitize to effects radiation. Methods LNCaP-COX-2 LNCaP-Neo were treated 0 1000 μM diclofenac. Next, a clonogenic assay was...
The naturally occurring amino acid 5-aminolevulinic (5-ALA) is a precursor of protoporphyrin IX (PpIX) biosynthesised in the mitochondria. When accumulated PpIX excited by light (wavelength 625-635 nm), reactive oxygen species (ROS) are generated. Here, we investigated whether 5-ALA may increase sensitisation prostate cancer (PCA) cells to radiotherapy through generation ROS via its metabolite, PpIX.Effect on PC-3 and DU-145 PCA cell lines treated with ionising radiation (IR) was examined...
Abstract Background The phase 3 open-label KEYNOTE-426 study demonstrated that first-line pembrolizumab plus axitinib improved overall survival (OS) and progression-free (PFS) versus sunitinib for metastatic renal cell carcinoma (mRCC) in a global population. This subgroup analysis investigated the efficacy safety of pembrolizumab-axitinib patients enrolled East Asia (Japan, South Korea, Taiwan). Methods Adults with clear mRCC were randomly assigned 1:1 to receive intravenous 200 mg every...
Activating mutation of the fibroblast growth factor receptor-3 (FGFR3) gene is known as a key molecular event in both oncogenesis and cell proliferation low-grade noninvasive human bladder urothelial carcinoma (UC), which characterized by frequent intravesical recurrence. In this study, we investigated antitumor potentiality 1-<i>tert</i>-butyl-3-[6-(3,5-dimethoxy-phenyl)-2-(4-diethylamino-butylamino)-pyrido[2,3-<i>d</i>]pyrimidin-7-yl]-urea (PD173074), small-molecule FGFR3-selective...
The ALKBH family of proteins are highly expressed in various types human cancer where they involved tumor growth and progression. However, multiple isoforms exist the effect individual on development urinary bladder is unknown, particularly molecular mechanisms progression from a noninvasive to invasive phenotype. We examined role function ALKBH2 vitro provide first report that suppression urothelial carcinoma cell line, KU7, reduces expression transmembrane mucin protein, MUC1, induces G1...
Abstract Increasing evidence suggests that tumour‐initiating cells ( TICs ) contribute to the development of prostate cancer. Here, we identified syndecan‐1 as a key molecule maintaining stability cancer . Holoclones harbouring biological properties stemness were derived from single‐cell cultures PC3 human cell line. These holoclones over‐expressed syndecan‐1, but showed reduced expression NADPH oxidase NOX and synthesis hydrogen peroxide oxygen radicals. Stable RNA ‐mediated silencing gene...
Objective To determine the prognostic factors of primary T1 high‐grade bladder cancer and to validate Spanish Urological Club for Oncological Treatment model in Japanese patients with treated at a single institution. Methods Records 106 from 1998 2013 were retrospectively reviewed. Variables included various clinicopathological parameters, including lymphovascular invasion tumor growth pattern front. Recurrence‐free survival progression‐free analyzed. Multivariate Cox proportional regression...
Collagen type 4 alpha 1 ( COL 4A1) and collagen 13 13A1) produced by urothelial cancer cells support the vital oncogenic property of tumor invasion. We investigated diagnostic prognostic capability 4A1 13A1 in voided urine compared observed values with those fragments cytokeratin‐19 CYFRA 21‐1), nuclear matrix protein 22 NMP ‐22), cytology bladder BC a). collected samples from 154 patients newly diagnosed a, before surgery 61 control subjects. Protein levels 4A1, 13A1, 21‐1, ‐22 supernatants...
Rationale:The expression of the chemokine (C-X-C motif) ligand 1 (CXCL1), an inflammatory protein, has been reported to be up-regulated in many human cancers.The mechanisms through which aberrant cellular CXCL1 levels promote specific steps tumor growth and progression are unknown.Methods: We described anticancer effects mechanism action HL2401, a monoclonal antibody directed at with vitro vivo data on bladder prostate cancers.Results: HL2401 inhibited proliferation invasion cells along...