A5036402119- Hydraulic flow and structures
- Hydrology and Sediment Transport Processes
- Nuclear Engineering Thermal-Hydraulics
- Hydrology and Watershed Management Studies
- Nuclear reactor physics and engineering
- Hydraulic and Pneumatic Systems
- Nuclear Materials and Properties
- Fluid Dynamics and Turbulent Flows
- Water Systems and Optimization
- Fluid Dynamics and Vibration Analysis
- Fungal and yeast genetics research
- Dam Engineering and Safety
- Sulfur Compounds in Biology
- Irrigation Practices and Water Management
- Bacterial Genetics and Biotechnology
- Biofuel production and bioconversion
- Amino Acid Enzymes and Metabolism
- Fungal Biology and Applications
- Enzyme Structure and Function
- Polyamine Metabolism and Applications
- Flood Risk Assessment and Management
- Enzyme Production and Characterization
- Folate and B Vitamins Research
- Microbial Metabolic Engineering and Bioproduction
- Gut microbiota and health
University of Tsukuba
2017-2024
Nihon University
2008-2023
Tsukuba International University
2019
Japan Atomic Energy Agency
1998-2018
Nara Institute of Science and Technology
2008-2017
Hamamatsu Photonics (Japan)
1998-2009
Shimadzu (Japan)
2005-2007
Tokyo Institute of Technology
2003-2006
Polytechnic University of Bari
2005
Advanced Science Research Center
2002
Skimming flows in stepped channels are systematically investigated under a wide range of channel slopes (5.7°⩽θ⩽55°). The flow conditions skimming classified into two regimes, and the hydraulic required to form quasi-uniform determined. An aerated depth is estimated from assumption that residual energy at end coincides with toe jump formed immediately downstream channel. In region, friction factor represented by relative step height slope. for slope θ=19° appears have maximum. formulated...
Abstract The relationships between depression and gut microbiota, particularly those involving the immune system, have become a major focus of recent research. Here, we analyzed changes in microbiota their sulfur metabolites feces rat model using modified 14-day social defeat stress (SDS) paradigm. Our results showed that SDS increased fecal Lactobacillus reuteri correlation with ergothioneine levels at around day 11, which continued for least 1 month following administration. In vitro study...
Dams and weirs must be equipped with adequate flood-release facilities for a safe dissipation of the kinetic energy flow. With stepped spillway design, it is essential to accurately predict flow-resistance contribution steps. The authors investigate flow resistance skimming flows associated form losses. Recent laboratory experiments were systematically performed channel slopes ranging from 5.7° up 55° results are compared existing prototype data. provide better understanding basic patterns...
This paper presents a systematic investigation on the transition from supercritical to subcritical flow at an abrupt drop. Over wide range of experimental conditions various types have been classified and concept low high drops defined. When step is supercritical, direction main passing over can be decided momentum equation. The reason why are formed has explained. hydraulic required form their length characteristics clarified.
Abstract Background Escherichia coli has two L-cysteine biosynthetic pathways; one is synthesized from O -acetyl L-serine (OAS) and sulfate by synthase (CysK), another produced via S -sulfocysteine (SSC) OAS thiosulfate SSC (CysM). converted into sulfite an uncharacterized reaction. As thioredoxins (Trx1 Trx2) glutaredoxins (Grx1, Grx2, Grx3, Grx4, NrdH) are known as reductases of peptidyl disulfides, overexpression such might be a good way for improving production to accelerate the...
Intracellular thiols like L-cystine and play a critical role in the regulation of cellular processes. Here we show that Escherichia coli has two transporters, symporter YdjN ATP-binding cassette importer FliY-YecSC. These proteins import L-cystine, an oxidized product from periplasm to cytoplasm. The YdjN, which is expected be new member regulon, low affinity transporter (Km = 1.1 μM) mainly involved uptake outside as nutrient. E. only importers because ΔydjNΔyecS mutant cells are not...
Abstract Stepped chutes effectively dissipate the energy of supercritical flow steep channels. In general, stepped chute flows are characterized by self-aeration. For their hydraulic design, it is important to know aerated depth, velocity, air concentration, and specific energy. Herein, quasi-uniform skimming systematically investigated over a range angles from 19° 55° for wide step heights discharges. Air concentration velocity profiles presented, depth-averaged friction factor determined....
Abstract Ergothioneine (ERG), a unique thiol compound, is suggested to function as an antioxidant and cytoprotectant. Despite several recent attempts produce ERG using various organisms, its yield was still very low the costs remained high. Since level of produced depends strictly on availability three distinct precursor amino acids ( l -cysteine (Cys), -histidine, -methionine (Met)), metabolic engineering for enhancement flux toward biosynthesis required. Herein, we took advantage high-Cys...
The transition from supercritical to subcritical flow in sloping channels is systematically investigated under a wide range of slopes. hydraulic conditions for the formation D‐jump, where entire jump formed on slope, and B‐jump, partly portion horizontal channel portion, are determined. Also, practical equations sequent depths length jumps proposed. For streamwise decay maximum velocity zone, analogy with wall jet discussed.
Abstract This paper presents the upper limit of inflow Froude number for undular-jump formations in smooth rectangular channels. It has been found that formation undular jumps depends not only on but boundary-layer development at toe jump under conditions which effects aspect ratio and Reynolds flow condition are negligible. The velocity first wave crest immediately before breaking is a maximum near water surface becomes critical velocity. For with developing inflow, F1limit shown...
Assuming that skimming flow occurs when the jet leaving a step has slope equal to of stepped spillway it impinges on pool behind next step, an equation been developed predict incipient value \Iy\dc/\ih, where \iy\dc = critical depth and \ih height. This was found agree well with most experimental observations for \ih/\il greater than about 1.0, \il length. For 1.7, \iy\dc/\ih 0.24, whereas values smaller 0.8, is almost constant at 0.8.
Abstract Background During the bread-making process, industrial baker's yeast, mostly Saccharomyces cerevisiae , is exposed to baking-associated stresses, such as air-drying and freeze-thaw stress. These stresses exert severe injury yeast cells, mainly due generation of reactive oxygen species (ROS), leading cell death reduced fermentation ability. Thus, there a great need for strain with higher tolerance stresses. Recently, we revealed novel antioxidative mechanism in laboratory that...
Ergothioneine (ERG) is a histidine-derived thiol compound suggested to function as an antioxidant and cytoprotectant in humans. Therefore, experimental trials have been conducted applying ERG from mushrooms dietary supplements cosmetic additive. However, this method of producing expensive; therefore, alternative methods for supply are required. Five Mycobacterium smegmatis genes, egtABCDE, confirmed be responsible biosynthesis. This enabled us develop practical fermentative production by...
To establish a reliable and practical ergothioneine (ERG) supply, we employed fermentative ERG production using Aspergillus oryzae, fungus used for food production. We heterologously overexpressed the egt-1 -2 genes of Neurospora crassa in A. oryzae succeeded producing (231.0 mg/kg media, which was 20 times higher than wild type). Abbreviations: ERG: ergothioneine; HER: hercynine; Cys-HER: hercynylcysteine-sulfoxide; SAM: S-adenosylmethionine; SAH: S-adenosylhomocysteine; l-His: l-histidine;...
Noninvasive biomarkers are urgently needed for optimal management of nonalcoholic fatty liver disease (NAFLD) the prevention progression into steatohepatitis (NASH) and hepatocellular carcinoma (HCC). In order to identify biomarkers, we generated swine (HCC) model associated with NAFLD performed serum proteomics on model.
Zinc (Zn) is an essential micronutrient for various physiological and metabolic processes in plants, although it toxic excess. To understand better Zn-responsive proteins, we developed a highly improved method of isoelectric focusing (IEF) which whole lysate from Arabidopsis roots subjected to IEF without any desalting steps. In this method, samples extracted with lysis buffer containing 1.5% SDS can also be directly applied IEF. By applying Zn-treated roots, 10 up-regulated 17...