- Enzyme Production and Characterization
- Biofuel production and bioconversion
- Gut microbiota and health
- Probiotics and Fermented Foods
- Obesity, Physical Activity, Diet
- Cardiovascular Health and Risk Factors
- Enzyme Catalysis and Immobilization
- Microbial Metabolic Engineering and Bioproduction
- Health disparities and outcomes
- Enzyme Structure and Function
- Metabolomics and Mass Spectrometry Studies
- Privacy, Security, and Data Protection
- Genomics and Phylogenetic Studies
- Microbial Metabolites in Food Biotechnology
- Blood Pressure and Hypertension Studies
- Maternal Mental Health During Pregnancy and Postpartum
- Polysaccharides Composition and Applications
- COVID-19 and Mental Health
- Nutritional Studies and Diet
- Phytase and its Applications
- Clostridium difficile and Clostridium perfringens research
- Acute Ischemic Stroke Management
- Protein Hydrolysis and Bioactive Peptides
- Child Nutrition and Water Access
- Opinion Dynamics and Social Influence
Yunnan Center for Disease Control And Prevention
2013-2025
Xi'an Jiaotong University
2022-2025
Dali University
2022-2024
Kunming Medical University
2022-2024
Inner Mongolia Medical University
2024
Yunnan Normal University
2012-2021
Kunming University of Science and Technology
2016
Beijing University of Posts and Telecommunications
2014-2015
The animal gastrointestinal tract contains a complex community of microbes, whose composition ultimately reflects the co-evolution microorganisms with their host. An analysis 78,619 pyrosequencing reads generated from pygmy loris fecal DNA extracts was performed to help better understand microbial diversity and functional capacity gut microbiome. taxonomic metagenomic indicated that microbiomes were dominated by Bacteroidetes Proteobacteria phyla. hierarchical clustering several metagenomes...
The widely used plasticizer phthalate esters (PAEs) have become a public concern because of their effects on environmental contamination and toxicity mammals. However, the biodegradation PAEs, especially diisobutyl (DiBP), remains poorly understood. In particular, genes involved in hydrolysis these compounds were not conclusively identified. this study, CarEW gene, which encodes an enzyme that is capable hydrolyzing ρ-nitrophenyl fatty acids, was cloned from thermophilic bacterium Bacillus...
The animal gastrointestinal tract contains a complex community of microbes, whose composition ultimately reflects the co-evolution microorganisms with their host and diet adopted by host. Although importance gut microbiota humans has been well demonstrated, there is paucity research regarding non-human primates (NHPs), especially herbivorous NHPs.In this study, an analysis 97,942 pyrosequencing reads generated from Rhinopithecus bieti fecal DNA extracts was performed to help better...
Heart disease has been the leading cause of death in China since 2020. With development aging population, dietary habits changes, and extreme climate conditions, this study aimed to evaluate national sub-national changes heart burden from 2013 2021 china, using data National Mortality Surveillance System. This was mainly System, which covered 300 million, accounted for 24% China's with provincial representativeness 2021. The categories were modified contain individual classes disease,...
Bo Xu , Liming Dai Junjun Li Meng Deng Huabiao Miao Junpei Zhou Yuelin Mu Qian Wu Xianghua Tang Yunjuan Yang Junmei Ding Nanyu Han and Zunxi Huang . J. Microbiol. Biotechnol. 2016;26:9-19. https://doi.org/10.4014/jmb.1504.04021
A novel bacterium, Bacillus licheniformis K-19, which produces a large amount of akeratinase that is extremely thermostable and has broad resistance to pH, was isolated and characterized. The maximum keratinase activity (about 224 Uml-1) produced at 37°C when the bacterium cultured for 72 h in broth containing feather meal with initial pH 7.5. The observed over wide range temperatures (30 - 90°C) and values (pH 6 10). It was optimal at 60°C and pH 7.5 8 respectively. These results suggest...
Phytase supplied in feeds for monogastric animals is important improving nutrient uptake and reducing phosphorous pollution. High-thermostability phytases are particularly desirable due to their ability withstand transient high temperatures during feed pelleting procedures. A comparison of crystal structures the widely used industrial Aspergillus niger PhyA phytase (AnP) with its close homolog, thermostable fumigatus (AfP), suggests 18 residues three segments associated thermostability. In...
Abstract Background We aim to describe the prevalence and trends of hypertension among 9–17 school-aged students from 2017 2019 in Yunnan, China. Methods conducted a cross-sectional study by using data Students’ Health Surveillance Surveys 2017, 2018 Yunnan. The Yunnan collected date on physical fitness health status through multistage-stratified sampling 3 prefectures. In each prefecture, population were classified gender region (urban or rural), group had an equal size. Diagnosing criteria...
China is experiencing rapid population aging, with the proportion of older adult people aged 60 and above reaching 19. 8% total in 2022. With growth age, physical function adults declines their mental health getting worse, increasing trend empty nesting childlessness, lack information social interaction others fall into isolation, loneliness some problems, problems gradually rises mortality rate increases incrementally, requiring effective ways to intervene promote healthy aging.The present...
Bos frontalis, which consumes bamboo and weeds, may have evolved unique gastrointestinal microorganisms that digest cellulase. A Paenibacillus sp. YD236 strain was isolated from B. frontalis feces, a GH8 endoglucanase gene, pglue8 (1107 bp, 54.5 % GC content), encoding 368-residue polypeptide (PgluE8, 40.4 kDa) cloned. PgluE8 efficiently hydrolyzed barley-β-d-glucan followed by CMC-Na, soluble starch, laminarin, glucan black yeast optimally at pH 5.5 50 °C, retained 78.6, 41.6, 34.5 maximum...
Yunnan province borders Myanmar, Laos, and Vietnam, giving it one of the longest in China. We aimed to determine trends prevalence impact COVID-19 on depressive symptoms among adolescents (12-18 years) from 2018 2022 Yunnan, southwest
Deacetylation of 7-aminocephalosporanic acid (7-ACA) at position C-3 provides valuable starting material for producing semisynthetic β-lactam antibiotics. However, few enzymes have been characterized in this process before now. Comparative analysis the genome thermophilic bacterium Alicyclobacillus tengchongensis revealed a hypothetical protein (EstD1) with typical esterase features. The EstD1 was functionally cloned, expressed, and purified from Escherichia coli BL21(DE3). It indeed...
A two-stage process was used to prepare rice alcohol, i.e., saccharification of glutinous by Rhizopus nigricans Q3, followed Saccharomyces cerevisiae's fermentation. Q3 cultured during the stage, and cerevisiae GY-1 added in fermentation stage. Total sugar content reducing these two stages were analyzed. The relationship between production proportion consumption interval analyzed using indices. It is an important rule that high-concentration syrup oligosaccharides prepared could reach 42°Bx...
Objective: Previous research has shown a high rate and unique pattern of suicide in China. We aim to present the current rates patterns Yunnan province, multi-ethnic region Southwestern Methods: This is descriptive study based on 3rd Chinese national mortality survey. reported by sex, 5-year age group, (urban or rural), minority group from 2004 2005 province. Results: estimated mean annual 19.82 per 100,000 total 8751 deaths year. Sex-specific estimates were 21.09 18.46 for males females,...
Stroke is an acute cerebrovascular disease with high mortality and disability. This study aimed to investigate the trend of stroke prevalence from 1989 2015 in China, explore transition high-risk population factors, provide some evidence develop more targeted intervention strategies. We derived baseline data China Health Nutrition Survey (CHNS). Participants responded face-to-face interviews examinations containing demographic information, behavioral health history, physical examination....
Catechol 1,2-dioxygenase is the key enzyme that catalyzes cleavage of aromatic ring catechol. We explored genetic diversity catechol in fecal microbial metagenome by PCR with degenerate primers. A total 35 gene fragments C12O were retrieved from DNA feces pygmy loris. Based on phylogenetic analysis, most sequences closely related to Acinetobacter. full-length was directly cloned, heterologously expressed Escherichia coli, and biochemically characterized. Purified catPL12 had optimum pH...
There is a huge variability in reducing sugars, viscosity, and composition of oligosaccharides the hydrolyzed products konjac flour with different concentrations. We analyzed factors affecting average degree polymerization (DP) during preparation from by β-mannanase under high-concentration solute hydrolysis model. Hydrolysate flour, using concentrations ranging 50 to 200 g/L, was directly added into 20 U/mL solution. The results showed that when proportion water content solution decreased,...