A5088675703- Sepsis Diagnosis and Treatment
- Immune Response and Inflammation
- Adrenal Hormones and Disorders
- Metabolomics and Mass Spectrometry Studies
- Rheumatoid Arthritis Research and Therapies
- Intensive Care Unit Cognitive Disorders
- COVID-19 Clinical Research Studies
- Long-Term Effects of COVID-19
- Systemic Lupus Erythematosus Research
- Inflammation biomarkers and pathways
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- S100 Proteins and Annexins
- Neonatal and Maternal Infections
- T-cell and B-cell Immunology
- Immune Cell Function and Interaction
- Heart Failure Treatment and Management
- SARS-CoV-2 and COVID-19 Research
- Molecular Biology Techniques and Applications
- Immunotherapy and Immune Responses
- Nosocomial Infections in ICU
- Chemokine receptors and signaling
- Radiomics and Machine Learning in Medical Imaging
- Pediatric Pain Management Techniques
- NF-κB Signaling Pathways
- Kawasaki Disease and Coronary Complications
bioMérieux (France)
2013-2025
Université Claude Bernard Lyon 1
2009-2025
Hospices Civils de Lyon
2010-2025
Hôpital Edouard Herriot
2006-2017
Laboratoire de Chimie
2015
bioMérieux (Brazil)
2010-2014
HCL Technologies (India)
2014
Inserm
2011
bioMérieux (United States)
2011
A dramatic decrease in circulating lymphocyte number is regularly described after septic shock. However, it unknown how early this alteration develops diagnosis of shock and if remains stable over time. Twenty-one patients with no comorbidities were included within 2 h the beginning vasopressive treatment. Flow cytometry phenotyping leukocyte subpopulations quantitative real-time polymerase chain reaction T-bet, GATA-3, FOXP3, RORγ mRNA performed from every 6 during subsequent 48 h. From...
Severe septic syndromes deeply impair innate and adaptive immunity are responsible for sepsis-induced immunosuppression. Although neutrophils represent the first line of defense against infection, little is known about their phenotype functions a few days after sepsis, when immunosuppressive phase maximal (i.e., between d 3 8). The objective present study was to perform, time, global evaluation neutrophil alterations in immunosuppressed patients (at 3–4 6–8) using phenotypic functional...
IL-17A is implicated in rheumatoid arthritis (RA) pathogenesis; however, the contribution of IL-17F remains to be clarified. Using microarrays and gene-specific expression assays, we compared regulatory effects alone or combination with TNF-alpha on RA synoviocytes. was studied osteoarthritis synovium by immunohistochemistry. The comparison between stimulatory effect synoviocytes assessed at protein level ELISA mRNA real-time RT-PCR. TNFRII RT-PCR immunofluorescence, neutralizing Ab used...
As early and appropriate care of severe septic patients is associated with better outcome, understanding the very first events in disease process needed. Pan-genomic analyses offer an interesting opportunity to study global genomic response within hours after sepsis. The objective this was investigate systemic intensive unit (ICU) determine whether patterns gene expression could be clinical severity evaluated by score.Twenty-eight ICU were enrolled at onset shock. Blood samples collected 30...
Abstract IL-17A is a cytokine secreted by the newly described Th17 cells implicated in rheumatoid arthritis (RA). Less known about its receptors synoviocytes. IL-17RA and IL-17RC were found to be overexpressed RA peripheral whole blood their expression was detected locally synovium. In vitro, synergized with TNF-α induce IL-6, IL-8, CCL-20, matrix metalloproteinase-3. Using microarrays, specific up-regulation of Glu-Leu-Arg+ CXC chemokines observed IL-17A-treated both posttranslational...
Although it is known that septic shock rapidly induces immune dysfunctions, which contribute to the impaired clearance of microorganisms observed in patients, mechanisms for this phenomenon remain incompletely understood. We recently observed, a microarray study, an altered circulating leukocyte CX3CR1 mRNA expression associated with patients' mortality. As monocytes play central role pathophysiology and express high levels CX3CR1, we therefore further investigated alteration its ligand...
Abstract Introduction Septic syndromes remain the leading cause of mortality in intensive care units (ICU). patients rapidly develop immune dysfunctions, intensity and duration which have been linked with deleterious outcomes. Decreased mRNA expressions major histocompatibility complex (MHC) class II-related genes reported after sepsis. We investigated whether their levels whole blood could predict septic shock patients. Methods A total 93 were included. On third day shock, five MHC ( CD74,...
In critically ill COVID-19 patients, the initial response to SARS-CoV-2 infection is characterized by major immune dysfunctions. The capacity of these severe patients mount a robust and persistent specific T cell despite presence alterations during ICU stay unknown. Critically were sampled five times 9 13 months afterwards. Immune monitoring included counts lymphocyte subpopulations, HLA-DR expression on monocytes, plasma IL-6 IL-10 concentrations, anti-SARS-CoV-2 IgG levels proliferation in...
Septic patients develop immune dysfunctions, the intensities and durations of which are associated with deleterious outcomes. LILRB2 (leukocyte immunoglobulin-like receptors subfamily B, member 2), an inhibitory LILR family receptors, is known for its immunoregulatory properties. In a microarray study, we identified as upregulated gene in septic shock patients. On monocytes primed LPS ex vivo, mRNA protein expressions were dose-dependently downregulated subsequently highly versus...
Intensive care unit (ICU)-acquired infections (IAI) result in increased hospital and ICU stay, costs mortality. To date, no biomarker has shown sufficient evidence ease of application clinical routine for the identification patients at risk IAI. We evaluated association systemic mRNA expression two host response biomarkers, CD74 IL10, with IAI occurrence a large cohort patients.ICU were prospectively enrolled multicenter study. Whole blood was collected on day admission (D1) 3 (D3) 6 (D6)...
The development of stratification tools based on the assessment circulating mRNA genes involved in immune response is constrained by heterogeneity septic patients. aim this study to develop a transcriptomic score pragmatic combination immune-related detected with prototype multiplex PCR tool.As training cohort, we used gene expression dataset obtained from 176 critically ill patients enrolled REALISM (NCT02638779) various etiologies and still hospitalized intensive care unit (ICU) at day...
Transcriptomics biomarkers have been widely used to predict mortality in patients with sepsis. However, the association between mRNA levels and outcomes shows substantial variability over course of sepsis, limiting their predictive performance. We aimed to: (a) identify validate an biomarker signature whose all-cause intensive care unit (ICU) is consistent at several timepoints; (b) evaluate how this could be lactate for prognostic enrichment conducted a gene expression analysis study two...
FoxO3a is a transcriptional factor implicated in cell cycle regulation and apoptosis. Since rheumatoid arthritis (RA) associated with apoptosis defects, the expression level, phosphorylation status of was investigated blood synovium from patients RA.In microarray experiments, an overexpression mRNA observed RA compared healthy controls. quantified polymorphonuclear cells (PMNs) peripheral mononuclear by qRT-PCR. Total phosphorylated (pFoxO3a) protein analysed leucocytes versus controls...
Chemokine (C-X3-C motif) receptor 1 (CX3CR1) was identified as the most differentially expressed gene between survivors and non-survivors in two independent cohorts of septic shock patients proposed a marker sepsis-induced immunosuppression. Whether such biomarker is associated with mortality heterogeneous group critically ill unknown. The primary objective this study to evaluate association CX3CR1 messenger RNA (mRNA) expression intensive care unit (ICU) patients. secondary similar...
Abstract Background Lymphopenia is a hallmark of severe coronavirus disease 19 (COVID-19). Similar alterations have been described in bacterial sepsis and therapeutic strategies targeting T cell function such as recombinant human interleukin 7 (rhIL-7) proposed this clinical context. As COVID-19 viral sepsis, the objectives study were to characterize lymphocyte response over time patients assess effect ex vivo administration rhIL-7. Results Peripheral blood mononuclear cells from...
<b>Objective:</b> To investigate the genetic contribution of cytokine gene polymorphisms (interleukin 1 (IL1) and tumour necrosis factor α (TNFα)) on disease phenotype response to TNF-blocking agents in a population patients with juvenile idiopathic arthritis (JIA). <b>Methods:</b> A cohort 107 consecutive JIA who were receiving treatment anti-TNF was enrolled this study. Analysis for IL1B +3954, IL1RA +2018, TNFα −238 −308 performed by enzyme-linked oligo sorbent assay, compared those...
The aim of this study was to assess the effect low-dose corticosteroid therapy in reducing shock duration after severe burn.A placebo-controlled, double-blind, randomized clinical trial (RCT) performed on two parallel groups burn intensive care unit (ICU). Patients were receive either or placebo for seven days. A corticotropin test at time randomization, before administration treatment dose. Thirty-two severely burned patients with refractory (>0.5 μg/kg/min norepinephrine) prospectively...
Septic shock remains a serious disease with high mortality and increased risk of hospital-acquired infection. The prediction outcome is the utmost importance for selecting patients therapeutic strategies aiming to modify immune response. aim this study was assess capability S100A9 messenger RNA in whole blood from septic predict survival occurrence infection.Cohort study.Two intensive care units university hospital.The included (n = 166) healthy volunteers 44).None.For patients, overall 38%...
Novel biomarkers are needed to progress toward individualized patient care in sepsis. The immune profiling panel (IPP) prototype has been designed as a fully-automated multiplex tool measuring expression levels of 26 genes sepsis patients explore functions, determine endotypes and guide personalized clinical management. performance the IPP gene set predict 30-day mortality not extensively characterized heterogeneous cohorts patients. Publicly available microarray data with widely variable...
<b>Objective:</b> To investigate whether ethnic differences exist in the effect of shared epitope and selected cytokine gene polymorphisms on susceptibility severity rheumatoid arthritis Syria (Damascus) France (Rhône-Alpes area). <b>Methods:</b> 156 patients with 120 healthy controls from were compared 512 471 France. Shared status, interleukin (IL)-1B +3954, IL-1RN +2018 tumour necrosis factor α promoter (−238 −308) analysed by enzyme-linked oligosorbent assay. Joint destruction was...
Abstract Background The objective of this study was to evaluate the ability endothelial biomarkers early predict clinical deterioration patients admitted emergency department (ED) with a suspected sepsis. This prospective, multicentre, international conducted in EDs. Adult acute bacterial infection and sepsis were enrolled but only those confirmed analysed. kinetics organ dysfunction collected at T0, T6 T24 hours after ED admission assess prognostic performances sVEGFR2, suPAR procalcitonin...
Despite shortening vasopressor use in shock, hydrocortisone administration remains controversial, with potential harm to the immune system. Few studies have assessed impact of on transcriptional response and we are lacking data burn shock. Our objective was assess hydrocortisone-induced modulation severe particularly response. We collected whole blood samples during a randomized controlled trial assessing efficacy Using genome microarrays, first compared patients (n = 32) from placebo group...
Nucleic acid sequence-based amplification (NASBA) is a sensitive isothermal transcription-based method known to be suitable tool for RNA research. We demonstrate that NASBA technology can applied single nucleotide polymorphism (SNP) analysis using human genomic DNA as template. Combination of with multiplex hybridization specific molecular beacons makes it possible unambiguously discriminate the presence SNP interest. This protocol easy-to-use, robust, and rapidly detect substitutions in...