A5038257875- Sepsis Diagnosis and Treatment
- Intensive Care Unit Cognitive Disorders
- Nosocomial Infections in ICU
- Antibiotics Pharmacokinetics and Efficacy
- Cardiac, Anesthesia and Surgical Outcomes
- Immune Response and Inflammation
- Respiratory Support and Mechanisms
- Pneumonia and Respiratory Infections
- Antibiotic Resistance in Bacteria
- Phagocytosis and Immune Regulation
- Cardiac Arrest and Resuscitation
- Antibiotic Use and Resistance
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Intraperitoneal and Appendiceal Malignancies
- Appendicitis Diagnosis and Management
- Advanced Glycation End Products research
- Healthcare Systems and Practices
- Bacterial Identification and Susceptibility Testing
- Trauma, Hemostasis, Coagulopathy, Resuscitation
- Airway Management and Intubation Techniques
- Frailty in Older Adults
- Trauma and Emergency Care Studies
- Hemodynamic Monitoring and Therapy
- COVID-19 and healthcare impacts
- Central Venous Catheters and Hemodialysis
Hôpital Lyon Sud
2016-2025
Hospices Civils de Lyon
2016-2025
Centre International de Recherche en Infectiologie
2017-2025
Centre National de la Recherche Scientifique
2017-2025
Inserm
2015-2025
Université Claude Bernard Lyon 1
2016-2025
École Normale Supérieure de Lyon
2017-2024
Omar Al-Mukhtar University
2023
Lyon College
2023
Christie's
2023
Uncontrolled extracellular matrix production by fibroblasts in response to tissue injury contributes fibrotic diseases, such as idiopathic pulmonary fibrosis (IPF), a progressive and ultimately fatal process that currently has no cure. Although dysregulation of miRNAs is known be involved variety pathophysiologic processes, the role lung diseases unclear. In this study, we found up-regulation miR-21 lungs mice with bleomycin-induced also patients IPF. Increased expression was primarily...
Toll-like receptors (TLRs) are major that enable inflammatory cells to recognize invading microbial pathogens. MicroRNAs small non-coding RNAs play important regulatory roles in a variety of biological processes. In this study, we found microRNA, miR-147, was induced upon stimulation multiple TLRs and functioned as negative regulator TLR-associated signaling events murine macrophages. We first demonstrated the NMES1 transcript functional primary miR-147. miR-147 LPS-stimulated mouse...
Although metabolic conditions associated with an increased AMP/ATP ratio are primary factors in the activation of 5′-adenosine monophosphate-activated protein kinase (AMPK), a number recent studies have shown that intracellular levels reactive oxygen species can stimulate AMPK activity, even without decrease cellular ATP. We found exposure recombinant AMPKαβγ complex or HEK 293 cells to H<sub>2</sub>O<sub>2</sub> was activity and also resulted oxidative modification AMPK, including...
To determine whether peak systolic velocity of tricuspid annular motion assessed by tissue Doppler echocardiography (Sta), a right ventricular function parameter, can discriminate patients with true- and false-positive pulse pressure variation. Pulse variation is used to predict fluid responsiveness in mechanically ventilated patients. However, this parameter has been reported be falsely positive, especially dysfunction.A prospective study.Medical surgical intensive care unit university...
Severe septic syndromes deeply impair innate and adaptive immunity are responsible for sepsis-induced immunosuppression. Although neutrophils represent the first line of defense against infection, little is known about their phenotype functions a few days after sepsis, when immunosuppressive phase maximal (i.e., between d 3 8). The objective present study was to perform, time, global evaluation neutrophil alterations in immunosuppressed patients (at 3–4 6–8) using phenotypic functional...
Abstract Background Antimicrobial resistance (AMR) compromises the treatment of patients with serious infections in intensive care units (ICUs), and physicians are increasingly facing bacterial limited or no adequate therapeutic options. A survey was conducted to assess physicians’ perception AMR situation European Union/European Economic Area (EU/EEA). Methods Between May July 2017, working ICUs were invited complete an online questionnaire hosted by Society Intensive Care Medicine. The...
Rationale: Patients with a severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection are at higher risk of ventilator-associated pneumonia (VAP) and may have an increased attributable mortality (increased or decreased death if VAP occurs in patient) fraction (proportion deaths that to exposure) VAP-related compared subjects without disease (COVID-19). Objectives: Estimation the among patients COVID-19. Methods: Using REA-REZO surveillance network, three groups adult medical ICU...
Phagocytosis of apoptotic cells is critical to resolution inflammation. High mobility group box 1 protein (HMGB1), a mediator inflammation, has been shown diminish phagocytosis through binding phosphatidylserine (PS) exposed on the surface neutrophils. However, it currently unknown whether HMGB1 also modulates activity receptors involved in PS recognition phagocytes. In present studies, we found that preincubation macrophages with decreased their ability engulf neutrophils or thymocytes....
ObjectivesEnterococci may increase morbidity and mortality in elderly patients with intra-abdominal infections (IAIs) hospitalized the intensive care unit (ICU).
Abstract Clearance of apoptotic cells by macrophages and other phagocytic cells, called efferocytosis, is a central process in the resolution inflammation. Although receptor for advanced glycation end products (RAGE) has been shown to participate variety acute chronic inflammatory processes lungs organs, role RAGE efferocytosis not reported. In present studies, we examined potential involvement efferocytosis. Macrophages from transgenic RAGE−/− mice showed decreased ability engulf...
Abstract Introduction Septic syndromes remain the leading cause of mortality in intensive care units (ICU). patients rapidly develop immune dysfunctions, intensity and duration which have been linked with deleterious outcomes. Decreased mRNA expressions major histocompatibility complex (MHC) class II-related genes reported after sepsis. We investigated whether their levels whole blood could predict septic shock patients. Methods A total 93 were included. On third day shock, five MHC ( CD74,...
Low first-dose peak serum concentrations of amikacin and gentamicin are commonly reported in ICU patients. The present study aimed to assess whether 30 mg/kg or 8 achieved target patients with severe sepsis. Sixty-three (Simplified Acute Physiology Score II = 43 ± 16) sepsis an indication for intravenous (n 47) were included. first (30 amikacin; gentamicin) subsequent doses corresponding min after the completion infusion) recorded. French guideline ≥60 ≥30 mg/L gentamicin, respectively. A...
OBJECTIVES: Little is known about the epidemiology of ventilator-acquired pneumonia among coronavirus disease 2019 patients such as incidence or etiological agents. Some studies suggest a higher risk ventilator-associated in this specific population. DESIGN: Cohort exposed/nonexposed study REA-REZO surveillance network. SETTING: Multicentric; ICUs France. PATIENTS: The at admission were matched on age, sex, center inclusion, presence antimicrobial therapy admission, patient provenance, time...
Phagocytosis of apoptotic cells by macrophages, known as efferocytosis, is a critical process in the resolution inflammation. High mobility group box 1 (HMGB1) protein was first described nuclear nonhistone DNA-binding protein, but now to be secreted activated during inflammatory processes, where it participates diminishing efferocytosis. Although HMGB1 undergo modification when secreted, effect such modifications on inhibitory actions efferocytosis have not been reported. In present...
Abstract High mobility group box 1 (HMGB1) is a highly conserved protein with multiple intracellular and extracellular functions, including transcriptional regulation, as well modulation of inflammation, cell migration, ingestion apoptotic cells. In these experiments, we examined potential role for HMGB1 in modulating phagocytosis. We found that phagocytosis cells resulted translocation into the cytoplasm space. Transient or stable inhibition expression bone marrow-derived macrophages...
Patients with advanced chronic obstructive pulmonary disease (COPD) are at risk for developing invasive aspergillosis. A clinical algorithm has been validated to discriminate colonization from putative aspergillosis (PIPA) in Aspergillus-positive respiratory tract cultures of critically ill patients. We focused on patients COPD who met the criteria PIPA.This matched cohort study included two university hospital intensive care units (ICUs). studied factors PIPA as well impact short- and...
Septic patients develop immune dysfunctions, the intensities and durations of which are associated with deleterious outcomes. LILRB2 (leukocyte immunoglobulin-like receptors subfamily B, member 2), an inhibitory LILR family receptors, is known for its immunoregulatory properties. In a microarray study, we identified as upregulated gene in septic shock patients. On monocytes primed LPS ex vivo, mRNA protein expressions were dose-dependently downregulated subsequently highly versus...
Data on the relationship between antimicrobial resistance and mortality remain scarce, this needs to be investigated in intensive care units (ICUs). The aim of study was compare ICU rates patients with ICU-acquired pneumonia due highly antimicrobial-resistant (HAMR) bacteria those non-HAMR bacteria. We conducted a multicenter, retrospective cohort using French National Surveillance Network for Healthcare Associated Infection ICUs ("REA-Raisin") database, gathering data from 200 January 2007...
Abstract This retrospective study aimed to compare the mortality and burden of respiratory syncytial virus (RSV group), SARS-CoV-2 (COVID-19 non-H1N1 (Seasonal influenza group) H1N1 (H1N1 in adult patients admitted intensive care unit (ICU) with failure. A total 807 were included. Mortality was compared between four following groups: RSV, COVID-19, seasonal influenza, groups. Patients RSV group had significantly more comorbidities than other patients. At admission, COVID-19 less severe...