A5067682780- Sepsis Diagnosis and Treatment
- Immune Response and Inflammation
- Intensive Care Unit Cognitive Disorders
- Cytomegalovirus and herpesvirus research
- Machine Learning in Healthcare
- Liver Disease and Transplantation
- Chromosomal and Genetic Variations
- Organ Transplantation Techniques and Outcomes
- Inflammation biomarkers and pathways
- RNA and protein synthesis mechanisms
- Pneumonia and Respiratory Infections
- Liver Disease Diagnosis and Treatment
- COVID-19 Clinical Research Studies
- Immune Cell Function and Interaction
- Genetic diversity and population structure
- Long-Term Effects of COVID-19
- Genomics and Phylogenetic Studies
- RNA modifications and cancer
- Inflammasome and immune disorders
- Adrenal Hormones and Disorders
- Trauma, Hemostasis, Coagulopathy, Resuscitation
- Digital Imaging for Blood Diseases
- SARS-CoV-2 and COVID-19 Research
- Clinical Reasoning and Diagnostic Skills
- Acute Kidney Injury Research
Hospices Civils de Lyon
2019-2025
Université Claude Bernard Lyon 1
2019-2025
bioMérieux (France)
2019-2025
Hôpital Edouard Herriot
2019-2024
Institut Mérieux (France)
2024
Université de Limoges
2017
OBJECTIVES: The host response plays a central role in the pathophysiology of sepsis and severe injuries. So far, no study has comprehensively described overtime changes injury-induced immune profile large cohort critically ill patients with different etiologies. DESIGN: Prospective observational study. SETTING: Adult ICU University Hospital Lyon, France. PATIENTS: Three hundred fifty-three septic, trauma, surgical 175 healthy volunteers were included REAnimation Low Immune Status Marker...
In critically ill COVID-19 patients, the initial response to SARS-CoV-2 infection is characterized by major immune dysfunctions. The capacity of these severe patients mount a robust and persistent specific T cell despite presence alterations during ICU stay unknown. Critically were sampled five times 9 13 months afterwards. Immune monitoring included counts lymphocyte subpopulations, HLA-DR expression on monocytes, plasma IL-6 IL-10 concentrations, anti-SARS-CoV-2 IgG levels proliferation in...
Infections are a leading cause of early mortality after liver transplantation (LT). Prior to transplantation, cirrhosis-associated immune dysfunction significantly increases the risk infection. This study investigated potential monitoring, with focus on monocytic HLA-DR (mHLA-DR) expression, as predictor post-LT complications. We conducted prospective 130 patients awaiting LT at Lyon University Hospital assess mHLA-DR lymphocyte subsets, and T-cell function before LT. Multivariate analysis...
Elevated neutrophil to lymphocyte ratio (NLR) upon ICU admission has been reported be associated with disease progression, severity, or mortality in critically ill patients. However, the overtime trajectories of NLR after and their association other markers intensive care units patients’ immuno-inflammatory status have not evaluated so far. In a cohort 353 patients (sepsis, trauma, major surgery), we between deterioration (mortality occurrence nosocomial infection) both by conventional...
Sepsis is a life-threatening organ dysfunction caused by dysregulated host response to infection. The immune system plays key role in sepsis onset and remains over time heterogeneous manner. Here, we decipher the heterogeneity of first week evolution monocyte HLA-DR (mHLA-DR) surface protein expression septic patients, molecule for adaptive immunity onset. We found verified four distinctive trajectories endotypes discovery (n = 276) verification cohort 102). highlight that 59% patients...
The development of stratification tools based on the assessment circulating mRNA genes involved in immune response is constrained by heterogeneity septic patients. aim this study to develop a transcriptomic score pragmatic combination immune-related detected with prototype multiplex PCR tool.As training cohort, we used gene expression dataset obtained from 176 critically ill patients enrolled REALISM (NCT02638779) various etiologies and still hospitalized intensive care unit (ICU) at day...
Abstract Sepsis induces intense, dynamic and heterogeneous host response modulations. Despite improvement of patient management, the risk mortality healthcare-associated infections remains high. Treatments to counterbalance immune are under evaluation, but effective biomarkers still lacking perform stratification. The design present study was defined alleviate limitations existing literature: we selected patients who survived initial hyperinflammatory hospitalized at day 5–7 after ICU...
Abstract Background Acute kidney injury (AKI) is common in hospitalized patients and results significant morbidity mortality. The objective of the study was to explore systemic immune response intensive care unit presenting with AKI, especially association between profiles persistent AKI during first week after admission following various types injuries (sepsis, trauma, surgery, burns). Methods REALAKI an ancillary analysis REAnimation Low Immune Status Marker (REALISM) cohort study, which...
Intensive care unit (ICU) patients develop an altered host immune response after severe injuries. This may evolve towards a state of persistent immunosuppression that is associated with adverse clinical outcomes. The expression human leukocyte antigen DR on circulating monocytes (mHLA-DR) and ex vivo release tumor necrosis factor α (TNF-α) by lipopolysaccharide-stimulated whole blood are two related biomarkers offered to characterize this phenomenon. purpose study was concomitantly evaluate...
Novel biomarkers are needed to progress toward individualized patient care in sepsis. The immune profiling panel (IPP) prototype has been designed as a fully-automated multiplex tool measuring expression levels of 26 genes sepsis patients explore functions, determine endotypes and guide personalized clinical management. performance the IPP gene set predict 30-day mortality not extensively characterized heterogeneous cohorts patients. Publicly available microarray data with widely variable...
We described the HERV and MaLR transcriptome in PBMCs, finding that about 8.4% of LTR retrotransposon loci were expressed identifying betaretrovirus-like HERVs as those with highest percentage loci. found 4,607 modulated a result vivo stimulation LPS. The HERV-H group showed number differentially most intact proviruses. characterized expressed, checking their genomic context insertion observing general colocalization genes are involved immune response, consequence LPS stimulation. analyses...
Abstract Background The immune response of critically ill patients, such as those with sepsis, severe trauma, or major surgery, is heterogeneous and dynamic, but its characterization impact on outcomes are poorly understood. Until now, the primary challenge in advancing our understanding disease has been to concurrently address both multiparametric temporal aspects. Methods We used a clustering method identify distinct groups based various marker trajectories during first week after...
Abstract Sepsis triggers a complex response marked by the simultaneous presence of proinflammatory and immunosuppressive elements, disrupting mechanisms intended to maintain homeostasis. While NLRP3 inflammasome has been demonstrated contribute inflammatory side, its connection with delayed sepsis-induced immunosuppression remains unexplored. The present objective was concomitantly prospectively assess activation (IL-1β, IL-18, soluble receptors) features immune failure (IL-10, mHLA-DR,...
Immune responses affiliated with COVID-19 severity have been characterized and associated deleterious outcomes. These approaches were mainly based on research tools not usable in routine clinical practice at the bedside. We observed that a multiplex transcriptomic panel prototype termed Profiling Panel (IPP) could capture dysregulation of immune ICU patients admission. Nine transcripts mortality univariate analysis this 9-mRNA signature remained significantly multivariate included age, SOFA...
Abstract Background and Research Question The host response plays a central role in the pathophysiology of sepsis severe injuries. So far, no study has comprehensively described overtime changes injury-induced immune profile large cohort critically ill patients with different etiologies. Study Design Methods 353 septic, trauma surgical 175 healthy volunteers were prospectively included REAnimation Low Immune Status Marker (REALISM) study. Extensive profiling was performed by assessing...
<title>Abstract</title> <bold>Background</bold> Infections are a leading cause of early mortality after liver transplantation (LT). Prior to transplantation, cirrhosis-associated immune dysfunction significantly increases the risk infection. This study investigated potential monitoring, with focus on monocytic HLA-DR (mHLA-DR) expression, as predictor post-LT complications.<bold>Methods</bold> We conducted prospective 130 patients awaiting LT at Lyon University Hospital assess mHLA-DR...
Immune reconstitution after allogeneic-hematopoietic-stem-cell transplantation (allo-HSCT) is a complex and individual process. In this cross-sectional study, whole-blood (WB) immune functional assay (IFA) was used to characterize function by assessing immune-related gene/pathway alterations. The usefulness of tool in the context infection, 6 months transplantation, evaluated. Sixty allo-HSCT recipients at 10 healthy volunteers (HV) were included. WB stimulated standardized TruCulture tubes...
Abstract Human Endogenous Retroviruses (HERVs) and Mammalian apparent LTR-retrotransposons (MaLRs) are retroviral sequences that integrated into the germline cells millions year ago. Transcripts of these present in several tissues, their expression is modulated pathological conditions, although function remains often far from being understood. In this work, we focused on HERVs/MaLRs modulation a scenario immune system activation. We used public dataset Peripheral Blood Mononuclear Cells...