A5081949119- Forensic and Genetic Research
- Forensic Anthropology and Bioarchaeology Studies
- Epigenetics and DNA Methylation
- Forensic Fingerprint Detection Methods
- Circadian rhythm and melatonin
- Autopsy Techniques and Outcomes
- Paleopathology and ancient diseases
- Forensic Entomology and Diptera Studies
- Free Radicals and Antioxidants
- Molecular Biology Techniques and Applications
- Mitochondrial Function and Pathology
- Biosensors and Analytical Detection
- Environmental DNA in Biodiversity Studies
- Archaeology and ancient environmental studies
- Metabolism and Genetic Disorders
- Cultural Heritage Materials Analysis
- SARS-CoV-2 detection and testing
- Genomics and Chromatin Dynamics
- Metabolomics and Mass Spectrometry Studies
- Identification and Quantification in Food
- Advanced Glycation End Products research
- Digital and Cyber Forensics
- Genetics, Aging, and Longevity in Model Organisms
- Salivary Gland Disorders and Functions
- Genetics and Neurodevelopmental Disorders
New Jersey Institute of Technology
2022-2024
Smithsonian Institution
2015-2024
National Museum of Natural History
2013-2024
Laboratories of Analytical Biology
2024
Florida International University
2017-2021
Mercyhurst University
2017
Mangalam Research Center for Buddhist Languages
2015
Universidad de Oviedo
2008-2013
Instituto de Investigación Sanitaria del Principado de Asturias
2009-2012
Instituto de Biología Molecular y Celular de Plantas
2009
Age estimation from DNA methylation markers has seen an exponential growth of interest, not in the least forensic scientists. The current published assays, however, can still be improved by lowering number assay and providing more accurate models to predict chronological age. From literature we selected 4 age-associated genes (ASPA, PDE4C, ELOVL2, EDARADD) determined CpG levels 206 blood samples both deceased living individuals (age range: 0-91 years). This data was subsequently used compare...
Age estimation is a critical aspect of reconstructing biological profile in forensic sciences. Diverse biochemical processes have been studied their correlation with age, and the results driven DNA methylation to forefront as promising biomarker. methylation, an epigenetic modification, has extensively recent years for developing age models criminalistics anthropology. Epigenetic clocks, which analyze sites undergoing hypermethylation or hypomethylation individuals paved way improved...
Abstract: Ewing sarcoma, the second most frequent bone cancer type, affects mainly adolescents, who have a survival of 50% 5 yr after diagnosis. Current treatments include combination surgery, radiotherapy and chemotherapy, which present potential serious side effects. Melatonin, natural molecule without relevant effects, has been previously shown to induce cytotoxicity in SK‐N‐MC cells, sarcoma cell line. Here, we found that there is synergy antitumor effect when melatonin (50 μ m –1 )...
Melatonin is an endogenous indolamine, classically known as a light/dark regulator. Besides classical functions, melatonin has also showed to have wide range of antitumoral effects in numerous cancer experimental models. However, no definite mechanism been described explain the whole antineoplasic effects. Here we describe dual effect on intracellular redox state relation its antiproliferative vs cytotoxic actions cells. Thus, inhibition proliferation correlates with decrease reactive oxygen...
Body fluid identification plays a crucial role in criminal investigations. Because of their presence many cases, blood and semen are the most relevant body fluids forensic sciences. Based on antigen-antibody reactions binding unique proteins for each fluid, serological assays represent one rapid highly specific tests semen. Currently, few studies have assessed factors affecting by applying these assays. This work aimed to study effect different fabrics from clothes time since deposition...
Abstract: Incorporation of new therapeutic agents remains as a major challenge for treatment patients with malignant haematological disorders. Melatonin is an indolamine without relevant side effects. It has been shown previously to exhibit synergism several chemotherapeutic drugs in Ewing sarcoma cells by potentiating the extrinsic pathway apoptosis. also sensitizes human glioma against TRAIL increasing DR5 expression. Here, we report induction cell death melatonin lines through activation...
There are several methodologies available to estimate time since death based on different changes that a corpse undergoes after death. However, these methods imprecise due the decomposition process being affected by factors, principally temperature and humidity. Current trends for determination of Postmortem Interval (PMI) attempt PMI in quantifiable manner, chemical body, summarized field “thanatochemistry”. Although have improved estimates, additional research has been developed increase...
Alcohol use disorder (AUD) is a major component in the etiology of cognitive decline and dementia. Underlying mechanisms by which long-term alcohol abuse causes dysfunction include excessive oxidative stress inflammation brain, activated increased reactive oxygen/nitrogen species (ROS/RNS), advanced glycation end-products (AGEs) high-mobility group box 1 protein (HMGB1). In pilot study, we examine potential clinical value circulating biomarkers including ROS/RNS, HMGB1, soluble receptor for...
Despite recent advances in cancer therapy, the 5-year survival rate for Ewing's sarcoma is still very low, and new therapeutic approaches are necessary. It was found previously that melatonin induces cell death line, SK-N-MC, by activating extrinsic apoptotic pathway. Melatonin actions were analysed metabolic viability/survival assays, flow cytometry, quantitative PCR mRNA expression, western blot protein activation/expression electrophoretic mobility shift assay transcription factor...
Some studies have pointed to the relationship between mitochondrial DNA (mtDNA) mutations and age in different tissues, which are potentially interesting aging research forensic identification because they could help improve estimation of age-at-death. The present study aims evaluate mtDNA from dentin pulp their relation with age. Healthy erupted third molars were extracted individuals two Spanish populations, aged 20–70. When analyzing amplification hypervariable region 2 by real-time...
Abstract Parkinson's disease has been widely related to both apoptosis and oxidative stress. Many publications relate the loss of mitochondrial potential an apoptosis‐mediated cell death in different vivo vitro models this pathology. The present study used dopaminegic specific neurotoxin 1‐methyl‐4‐phenylpyridinium (MPP + ) on neuron‐like PC12 cells, which is a well‐accepted model disease. Results showed early increase oxidants, drives modulation c‐Jun N‐terminal kinase (JNK) AKT/mammalian...
Age-at-death estimation constitutes one of the key parameters for identification human remains in forensic investigations. However, applications anthropology, many current methods are not sufficiently accurate adult individuals, leading to chronological age estimates erring by ±10 years. Based on recent trends aging studies, DNA methylation has great potential as a solution this problem. there only few studies that have been published utilizing determine from remains. The aim present study...
The postmortem interval (PMI) is difficult to estimate in later stages of decomposition. There therefore a need develop reliable methodologies late PMI. This study aims assess whether there correlation between changes the mineral composition human teeth and estimation X-ray diffraction (XRD) attenuated total reflection Fourier transform infrared (ATR-FTIR) spectroscopy techniques were performed address this challenge. Forty healthy obtained from odontological clinics stored at different...
Lateral flow immunochromatographic (LFI) tests are widely used in both biomedical and forensic sciences for different applications. In sciences, their main use is to detect body fluids at crime scenes. However, there situations which the amount of potential biological evidence so low that DNA extraction favored with respect identification fluids. Here, an efficient quick protocol presented integrate detection through LFI from a sample swab buffer, providing complete characterization...
Abstract The concept of ion channels as membrane therapeutic targets and diagnostic/prognostic biomarkers has attracted growing attention. We therefore investigated the expression pattern clinical significance Kv3.4 potassium channel subunit during development progression head neck squamous cell carcinomas (HNSCCs). KCNC4 mRNA levels were determined by real‐time RT‐PCR in both HNSCC tissue specimens derived lines. protein was evaluated immunohistochemistry paraffin‐embedded from 84 patients...