A5017446338- Neuroinflammation and Neurodegeneration Mechanisms
- Tryptophan and brain disorders
- Stress Responses and Cortisol
- Immune Response and Inflammation
- Photosynthetic Processes and Mechanisms
- Cerebrospinal fluid and hydrocephalus
- Alzheimer's disease research and treatments
- Neuropeptides and Animal Physiology
- Bacterial Infections and Vaccines
- Growth Hormone and Insulin-like Growth Factors
- Neonatal and fetal brain pathology
- S100 Proteins and Annexins
- Eicosanoids and Hypertension Pharmacology
- Regulation of Appetite and Obesity
- Digestive system and related health
- Advanced Glycation End Products research
- Infectious Encephalopathies and Encephalitis
- Aquatic Ecosystems and Phytoplankton Dynamics
- Natural Antidiabetic Agents Studies
- Neuroscience and Neuropharmacology Research
- Alcohol Consumption and Health Effects
- Algal biology and biofuel production
- Cannabis and Cannabinoid Research
- Influenza Virus Research Studies
- Birth, Development, and Health
Instituto de Investigación Biomédica de Málaga
2017-2024
Hospital Regional Universitario de Málaga
2021-2024
Universidad de Málaga
2013-2021
It is known that microglia morphology and function are closely related, but only few studies have objectively described different morphological subtypes. To address this issue, parameters of microglial cells were analyzed in a rat model aseptic neuroinflammation. After the injection single dose enzyme neuraminidase (NA) within lateral ventricle (LV) an acute inflammatory process occurs. Sections from NA-injected animals sham controls immunolabeled with marker IBA1, which highlights...
Microglia are the resident macrophages in brain. Traditionally, two forms of microglia have been described: one considered as a resting/surveillant state which cells highly branched morphology, and another an activated they acquire de-ramified or amoeboid form. However, many studies describe intermediate microglial morphologies emerge during pathological processes. Since form function closely related, it is interest to correlate morphology with extent its activation. To address this issue,...
Abstract Short-term behavioral alterations are associated with infection and aid the recovery from sickness. However, concerns have raised that sustained disturbances after acute neuroinflammation could relate to neurological diseases in long run. We aimed explore medium- long-term rats, using a model based on intracerebroventricular administration of enzyme neuraminidase (NA), which is part some pathogenic bacteria viruses. Neurological assessments were performed 2 10 weeks injection NA,...
Neuraminidase (NA) is a sialidase present, among various locations, in the envelope/membrane of some bacteria/viruses (e.g., influenza virus), and involved infectiveness and/or dispersion. The administration NA within brain lateral ventricle represents model acute sterile inflammation. relevance Toll-like receptors TLR2 TLR4 (particularly those microglial cells) such process was investigated.Mouse strains deficient either (TLR2-/-) or (TLR4-/-) were used. injected ventricle, inflammatory...
Exposure of microglia to an inflammatory environment may lead their priming and exacerbated response future stimuli. Here we aimed explore hypothalamic its consequences on energy balance regulation. A model intracerebroventricular administration neuraminidase (NA, which is present in various pathogens such as influenza virus) was used induce acute neuroinflammation. Evidences primed were observed 3 months after NA injection, namely (1) a heightened located the arcuate nucleus vivo challenge...
Alcohol use disorder (AUD) is a major component in the etiology of cognitive decline and dementia. Underlying mechanisms by which long-term alcohol abuse causes dysfunction include excessive oxidative stress inflammation brain, activated increased reactive oxygen/nitrogen species (ROS/RNS), advanced glycation end-products (AGEs) high-mobility group box 1 protein (HMGB1). In pilot study, we examine potential clinical value circulating biomarkers including ROS/RNS, HMGB1, soluble receptor for...
Abstract The administration of microbial neuraminidase into the brain ventricular cavities rodents represents a model acute aseptic neuroinflammation. Ependymal cell death and hydrocephalus are unique features this model. Here we demonstrate that activated microglia participates in ependymal death. Co-cultures pure with cells (both obtained from rats) were performed, or lipopolysaccharide used to activate microglia. viability was unaltered absence inflammatory stimulus (neuraminidase...
Increasing evidence shows that hypothalamic dysfunction, insulin resistance, and weight loss precede progress along with the cognitive decline in sporadic Alzheimer's Disease (AD) sex differences. This study aimed to determine effect of oral dietary administration D-Chiro-inositol (DCI), an inositol used against resistance associated polycystic ovary, on occurrence metabolic disorders transgenic 5xFAD mouse model AD (FAD: Family Disease). DCI was administered from 6 10 months age male female...
Aims: Some central nervous system (CNS) pathogens express neuraminidase (NA) on their surfaces. In the rat brain, a single intracerebroventricular (ICV) injection of NA induces myelin vacuolation in axonal tracts. Here we explore nature, time course, and role complement this damage. Methods: The spatio-temporal analysis was performed by optical electron microscopy. Myelin basic protein (MBP)-positive area oligodendrocyte transcription factor (Olig2)-positive cells were quantified damaged...
Children with pregnancy-associated plasma protein-A2 (PAPP-A2) mutations resulting in low levels of bioactive insulin-like growth factor-1 (IGF1) and progressive postnatal retardation have improved velocity height following recombinant human (rh)IGF1 treatment. The present study aimed to evaluate whether Pappa2 deficiency pharmacological manipulation GH/IGF1 system are associated sex-specific differences growth-related signaling pathways. Plasma, hypothalamus, pituitary gland liver...
Innate immune memory explains the plasticity of responses after repeated stimulation, leading to either enhanced or suppressed responses. This process has been extensively reported in peripheral cells and also, although modestly, brain. Here we explored two relevant aspects brain priming: its persistence over time dependence on TLR receptors. For this purpose, used an experimental paradigm consisting applying inflammatory stimuli three months apart. Wild type, toll-like receptor (TLR) 4 TLR2...
Preclinical studies on the effects of abrupt cessation selective serotonin reuptake inhibitors (SSRIs), a medication often prescribed in alcohol use disorder (AUD) patients with depression, results consumption escalation after resuming drinking. However, potential neuroinflammatory component this remains unexplored despite immunomodulatory role serotonin. Here, we utilized rat model 14-daily administration SSRI fluoxetine (10 mg/kg/day) along self-administration deprivation to study...
Abstract Background. Patients with pregnancy-associated plasma protein-A2 ( PAPP-A2 ) mutations have progressive postnatal growth retardation and high circulating levels of IGF1 bound in ternary complexes. The present study aims to assess whether Pappa2 deficiency is associated sex-specific differences the main components complexes signaling pathways response low bioavailability. Methods. Plasma, hypothalamus, pituitary gland liver were analyzed constitutive ko/ko mice both sexes that...
Abstract Preclinical studies on the effects of abrupt cessation selective serotonin reuptake inhibitors (SSRIs), a medication often prescribed in alcohol use disorder (AUD) patients with depression, results consumption escalation after resuming drinking. However, potential neuroinflammatory component this remains unexplored despite immunomodulatory role serotonin. Here, we utilized rat model 14-daily administration SSRI fluoxetine (10 mg/kg/day) along self-administration deprivation to study...