A5021438456- Forensic and Genetic Research
- Molecular Biology Techniques and Applications
- Genetic diversity and population structure
- Race, Genetics, and Society
- Forensic Anthropology and Bioarchaeology Studies
- Genetic Associations and Epidemiology
- Archaeology and Historical Studies
- Genomics and Phylogenetic Studies
- Identification and Quantification in Food
- RNA and protein synthesis mechanisms
- Archaeology and ancient environmental studies
- Historical and Archaeological Studies
- Epigenetics and DNA Methylation
- Paleopathology and ancient diseases
- Demographic Trends and Gender Preferences
- Bacteriophages and microbial interactions
- Human-Animal Interaction Studies
- T-cell and B-cell Immunology
- Environmental DNA in Biodiversity Studies
- Immune Cell Function and Interaction
- Evolutionary Psychology and Human Behavior
- Criminal Law and Evidence
- Nutrition, Genetics, and Disease
- Ancient Mediterranean Archaeology and History
- Chromosomal and Genetic Variations
KU Leuven
2012-2024
Universitair Ziekenhuis Leuven
2011-2021
Institute of Archeology
2015
Dokuz Eylül University
2003
Newcastle University
2003
Center for Human Genetics
2000
Maastricht University
1994
Membrane cofactor protein (MCP; CD46) is a widely expressed transmembrane complement regulator. Like factor H it inhibits activation by regulating C3b deposition on targets. Factor mutations occur in 10–20% of patients with hemolytic uremic syndrome (HUS). We hypothesized that MCP could predispose to HUS, and we sequenced coding exons affected individuals from 30 families. were detected three families: deletion two amino acids (D237/S238) family 1 (heterozygous) substitution, S206P, families...
Nonrecombining Y-chromosomal microsatellites (Y-STRs) are widely used to infer population histories, discover genealogical relationships, and identify males for criminal justice purposes. Although a key requirement their application is reliable mutability knowledge, empirical data only available small number of Y-STRs thus far. To rectify this, we analyzed large 186 Y-STR markers in nearly 2000 DNA-confirmed father-son pairs, covering an overall 352,999 meiotic transfers. Following...
Zooarcheological evidence suggests that pigs were domesticated in Southwest Asia ∼8,500 BC. They then spread across the Middle and Near East westward into Europe alongside early agriculturalists. European either independently or more likely appeared so as a result of admixture between introduced wild boar. As result, boar mtDNA lineages replaced Eastern/Anatolian signatures subsequently indigenous domestic pig Anatolia. The specific details these processes, however, remain unknown. To...
Age estimation from DNA methylation markers has seen an exponential growth of interest, not in the least forensic scientists. The current published assays, however, can still be improved by lowering number assay and providing more accurate models to predict chronological age. From literature we selected 4 age-associated genes (ASPA, PDE4C, ELOVL2, EDARADD) determined CpG levels 206 blood samples both deceased living individuals (age range: 0-91 years). This data was subsequently used compare...
A nonisotopic oligotyping method using reverse dot blot hybridization was developed for HLA class II DQA1, DQB1, DPB1, DRB1, DRB3, DRB4, DRB5 alleles. The polymorphic second exon of the different genes amplified by polymerase chain reaction (PCR). For each gene DNA hybridized at stringent conditions to membrane‐bound sequence‐specific oligonucleotides (SSOs) and visualization positive signals done chemiluminescence. combination 11, 18, 23 31 SSOs designed identify 9/13 DQAI, 16/17 23/24 DPB1...
Relevant for various areas of human genetics, Y-chromosomal short tandem repeats (Y-STRs) are commonly used testing close paternal relationships among individuals and populations, male lineage identification. However, even the widely 17-loci Yfiler set cannot resolve populations completely. Here, 52 centers generated quality-controlled data 13 rapidly mutating (RM) Y-STRs in 14,644 related unrelated males from 111 worldwide populations. Strikingly, >99% 12,272 were completely individualized....
During the last few decades, a wealth of studies dedicated to human Y chromosome and its DNA variation, in particular Y-chromosome single-nucleotide polymorphisms (Y-SNPs), has led construction well-established phylogeny. Since recent advent new sequencing technologies, discovery additional Y-SNPs is exploding their continuous incorporation phylogenetic tree leading an ever higher resolution. However, large increasing amount information included “complete” phylogeny, which now already...
The polymerase chain reaction (PCR) technique has provided a substantial improvement for the detection and analysis of known genetic polymorphisms. Here, we describe application this method variable number tandem repeat (VNTR) sequences. With use unique oligonucleotide primers, flanking sequence, thermostable Taq DNA polymerase, hypervariable regions 3′ Ha-ras gene, apolipoprotein B 5′ to joining segments heavy-chain immunoglobulin gene could be amplified. Alleles up 2,000 bp visualized...
Recent evidence suggests that seeking out extra-pair paternity (EPP) can be a viable alternative reproductive strategy for both males and females in many pair-bonded species, including humans. Accurate data on EPP rates humans, however, are scant mostly restricted to extant populations. Here, we provide the first large-scale, unbiased genetic study of historical Western European human population based combining Y-chromosomal infer patrilineages with genealogical surname data, which reflect...
Due to the rapid progress of next-generation sequencing (NGS) facilities, an explosion human whole genome data will become available in coming years. These can be used optimize and increase resolution phylogenetic Y chromosomal tree. Moreover, exponential growth known lineages require automatic determination position individual based on SNP calling up date tree.We present automated approach, 'AMY-tree', which is able determine a chromosome using profile, independently from NGS platform...
Male-specific Y-chromosome (chrY) polymorphisms are interesting components of the DNA for population genetics. While single nucleotide (Y-SNPs) indicate distant evolutionary ancestry, short tandem repeats (Y-STRs) able to identify close familial kinships. Detailed chrY analysis provides thus both biogeographical background information as paternal lineage identification. The rapid advancement high-throughput massive parallel sequencing (MPS) technology in past decade has revolutionized...
Summary Y‐chromosomal short tandem repeats (Y‐STRs) are often used in addition to single‐nucleotide polymorphisms (Y‐SNP) detect subtle patterns a population genetic structure. There are, however, indications for Y‐STR haplotype resemblance across different subhaplogroups within haplogroup R1b1b2 (R‐M269) which may lead erosion the observation of pattern. Hence question arises whether haplotypes still informative beyond high‐resolution Y‐SNP genotyping studies. To address this question, we...
<title>Abstract</title> Predicting recent intercourse is crucial in forensic casework on sexual assaults. In this work, we assessed whether can be predicted based the vaginal microbiome and compared it to gold standard method of semen detection. Using a prediction model 3,043 women, was with 71% accuracy balanced cross-validation machine learning setting. Next, validated longitudinal intervention study tested assault cases. The developed predictor could accurately establish 82% Yet,...
Abstract Objective Evolutionary theory has shown that seeking out extrapair paternity (EPP) can be a viable reproductive strategy for both sexes in pair‐bonded species, also humans. As yet, estimates of the contemporary or historical EPP rate human population are still rare. In present study, we estimated Dutch over last 400 years and compared with those obtained other populations to determine evolutionary, cultural, socio‐demographic factors influence cuckoldry behavior. Methods We via...