A5037139071- NF-κB Signaling Pathways
- Lymphoma Diagnosis and Treatment
- Ubiquitin and proteasome pathways
- Immune Cell Function and Interaction
- Chronic Lymphocytic Leukemia Research
- Chronic Myeloid Leukemia Treatments
- Melanoma and MAPK Pathways
- Acute Myeloid Leukemia Research
- Cytokine Signaling Pathways and Interactions
- Cancer Research and Treatments
- Immune Response and Inflammation
- Acute Lymphoblastic Leukemia research
- T-cell and B-cell Immunology
- Glycosylation and Glycoproteins Research
- Protein Tyrosine Phosphatases
- Genomics and Chromatin Dynamics
- Genomic variations and chromosomal abnormalities
- Viral-associated cancers and disorders
- interferon and immune responses
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- T-cell and Retrovirus Studies
- Chromosomal and Genetic Variations
- Occupational and environmental lung diseases
- Cancer Genomics and Diagnostics
- Toxin Mechanisms and Immunotoxins
KU Leuven
2008-2024
VIB-KU Leuven Center for Brain & Disease Research
2018
VIB-KU Leuven Center for Cancer Biology
2009-2014
Vlaams Instituut voor Biotechnologie
1997-2011
Center for Human Genetics
1995-2011
Radboud University Nijmegen
2003
Radboud University Medical Center
2003
Ghent University Hospital
2003
Universität Hamburg
1999-2000
University of Toronto
2000
Abstract The Carma1-Bcl10-Malt1 signaling module bridges TCR to the canonical IκB kinase (IKK)/NF-κB pathway. Covalent attachment of regulatory ubiquitin chains Malt1 paracaspase directs IKK activation. Further, ubiquitin-editing enzyme A20 was recently suggested suppress T cell activation, but molecular targets for remain elusive. In this paper, we show that regulates strength and duration IKK/NF-κB response upon TCR/CD28 costimulation. By catalyzing removal K63-linked from Malt1, prevents...
A nonisotopic oligotyping method using reverse dot blot hybridization was developed for HLA class II DQA1, DQB1, DPB1, DRB1, DRB3, DRB4, DRB5 alleles. The polymorphic second exon of the different genes amplified by polymerase chain reaction (PCR). For each gene DNA hybridized at stringent conditions to membrane‐bound sequence‐specific oligonucleotides (SSOs) and visualization positive signals done chemiluminescence. combination 11, 18, 23 31 SSOs designed identify 9/13 DQAI, 16/17 23/24 DPB1...
Proper regulation of nuclear factor κB (NF-κB) transcriptional activity is required for normal lymphocyte function, and deregulated NF-κB signaling can facilitate lymphomagenesis. We demonstrate that the API2-MALT1 fusion oncoprotein created by recurrent t(11;18)(q21;q21) in mucosa-associated lymphoid tissue (MALT) lymphoma induces proteolytic cleavage NF-κB-inducing kinase (NIK) at arginine 325. NIK requires concerted actions both partners generates a C-terminal fragment retains resistant...
Enhanced Green Fluorescent Protein (EGFP) is the most commonly used live cell reporter despite a number of conflicting reports that it can affect physiology. Thus far, precise mechanism GFP-associated defects remained unclear. Here we demonstrate EGFP and fusion proteins inhibit polyubiquitination, posttranslational modification controls wide variety cellular processes, like activation kinase signalling or protein degradation by proteasome. As consequence, NF-κB JNK pathways are less...
Mucosa-associated lymphoid tissue 1 (MALT1) controls antigen receptor-mediated signalling to nuclear factor κB (NF-κB) through both its adaptor and protease function. Upon stimulation, MALT1 forms a complex with BCL10 CARMA1, which is essential for initial IκBα phosphorylation NF-κB translocation. Parallel induction of activity serves inactivate negative regulators signalling, such as A20 RELB. Here we demonstrate key role auto-proteolytic cleavage in B- T-cell receptor signalling. occurred...
Fibrosis represents the uncontrolled replacement of parenchymal tissue with extracellular matrix (ECM) produced by myofibroblasts. While genetic fate-tracing and single-cell RNA-Seq technologies have helped elucidate fibroblast heterogeneity ontogeny beyond to myofibroblast differentiation, newly identified populations remain ill defined, respect both molecular cues driving their differentiation subsequent role in fibrosis. Using an unbiased approach, we metalloprotease ADAMTS12 as a...
We have constructed a detailed map of the genomic region containing ETS-variant gene 6 (ETV6), involved in translocations and deletions associated with hematologic malignancies. Thirty-eight cosmids were characterized belonging to two contigs spanning 340 kb, an EcoRl restriction was developed. The gap between contigs, 2 kb size, closed by PCR. contain complete coding sequence 5' 3' UTRs ETV6. Eight exons accounting for ETV6 cDNA identified. helix-loop-helix (HLH) motif is coded 3 4, whereas...
The recurrent translocation t(11;18)(q21;q21) associated with mucosa-associated lymphoid tissue (MALT) lymphoma results in the expression of an API2.MALT1 fusion protein that constitutively activates NF-kappaB. first baculovirus IAP repeat (BIR) domain API2 and C terminus MALT1, which contains its caspase-like domain, are present all reported variants interact TRAF2 TRAF6, respectively, suggesting their contribution to NF-kappaB signaling by API2.MALT1. Also, involvement BCL10 has been...
Abstract The translocation t(11;18)(q21;q21) that generates an API2-MALT1 fusion protein is the most common structural abnormality among genetic defects reported in mucosa-associated lymphoid tissue (MALT)-type lymphomas, and its presence correlates with apparent lack of further instability or chromosomal imbalances. Hence, constitutive nuclear factor-κB (NF-κB) activation induced by considered essential for B-cell transformation. To examine role development lymphomagenesis, Eμ-API2-MALT1...
Genetic events underlying pathogenesis of nodal and extranodal marginal zone lymphoma are not completely understood. We report here a novel t(X;14)(p11.4;q32.33) identified in 4 cases: 2 with mucosa-associated lymphoid tissue lymphoma, one gastric diffuse large B-cell lymphoma. In all cases, evolved from previous auto-immune disorder. Fluorescence situ hybridization molecular studies showed that t(X;14), which is mediated by immunoglobulin heavy chain locus, targets the GPR34 gene at Xp11.4....
MALT1 is a central signaling component in innate and adaptive immunity by regulating NF-κB other key pathways different cell types. Activities of are mediated its scaffold protease functions. Because role lymphocyte activation proliferation, inhibition proteolytic activity high interest for therapeutic targeting autoimmunity certain lymphomas. However, recent studies showing that Malt1 protease-dead knock-in (Malt1-PD) mice suffer from autoimmune disease have somewhat tempered the initial...