A5036942871- Neurotransmitter Receptor Influence on Behavior
- Obsessive-Compulsive Spectrum Disorders
- Neuroendocrine regulation and behavior
- Receptor Mechanisms and Signaling
- Neural dynamics and brain function
- Autism Spectrum Disorder Research
- Stress Responses and Cortisol
- Neuropeptides and Animal Physiology
- Neuroscience and Neuropharmacology Research
- Memory and Neural Mechanisms
- Photoreceptor and optogenetics research
- Nuclear Receptors and Signaling
- Tryptophan and brain disorders
- Genetics and Neurodevelopmental Disorders
- Anxiety, Depression, Psychometrics, Treatment, Cognitive Processes
- Computational Drug Discovery Methods
- Nicotinic Acetylcholine Receptors Study
- Adipose Tissue and Metabolism
- Nerve injury and regeneration
- Eating Disorders and Behaviors
- Neurogenesis and neuroplasticity mechanisms
- Hypothalamic control of reproductive hormones
- Bipolar Disorder and Treatment
- Attention Deficit Hyperactivity Disorder
- Functional Brain Connectivity Studies
University of Washington
2020-2025
University of Pittsburgh
2015-2025
Center for Neurosciences
2022
Seattle University
2022
University of Maryland, Baltimore
2011-2015
National Institutes of Health
2015
VistaGen Therapeutics (United States)
2015
National Institute of Mental Health
2015
The forced swim test is a rodent behavioral used for evaluation of antidepressant drugs, efficacy new compounds, and experimental manipulations that are aimed at rendering or preventing depressive-like states. Mice placed in an inescapable transparent tank filled with water their escape related mobility behavior measured. straightforward to conduct reliably it requires minimal specialized equipment. Successful implementation the adherence certain procedural details minimization unwarranted...
The tail-suspension test is a mouse behavioral useful in the screening of potential antidepressant drugs, and assessing other manipulations that are expected to affect depression related behaviors. Mice suspended by their tails with tape, such position it cannot escape or hold on nearby surfaces. During this test, typically six minutes duration, resulting oriented behaviors quantified. valuable tool drug discovery for high-throughput prospective compounds. Here, we describe details required...
Currently approved antidepressant drug treatment typically takes several weeks to be effective. The noncompetitive N-methyl-d-aspartate (NMDA) receptor antagonist ketamine has shown efficacy as a rapid-acting of depression, but its use is associated with significant side effects. We assessed effects following blockade the glycineB co-agonist site NMDA receptor, located on GluN1 subunit, by selective full 7-chloro-kynurenic acid (7-Cl-KYNA), delivered systemic administration brain-penetrant...
Abstract Compulsive behaviors are a hallmark symptom of obsessive compulsive disorder (OCD). Striatal hyperactivity has been linked to behavior generation in correlative studies humans and causal rodents. However, the contribution two distinct striatal output populations treatment is unknown. These direct indirect pathway-projecting spiny projection neurons (SPNs) have classically thought promote or suppress actions, respectively, leading long-held hypothesis that increased relative pathway...
The tail-suspension test is a mouse behavioral useful in the screening of potential antidepressant drugs, and assessing other manipulations that are expected to affect depression related behaviors. Mice suspended by their tails with tape, such position it cannot escape or hold on nearby surfaces. During this test, typically six minutes duration, resulting oriented behaviors quantified. valuable tool drug discovery for high-throughput prospective compounds. Here, we describe details required...
Rationale: Current first-line treatments for stress-related disorders such as Major Depressive Disorder (MDD) act on monoaminergic systems and take weeks to achieve a therapeutic effect with poor response low remission rates. Recent research has implicated the GABAergic system in pathophysiology of depression, including deficits interneurons targeting dendritic compartment cortical pyramidal cells. Objectives: The present study evaluates whether SH-053-2'F-R-CH3 (denoted "α5-PAM"), positive...
The forced swim test is a rodent behavioral used for evaluation of antidepressant drugs, efficacy new compounds, and experimental manipulations that are aimed at rendering or preventing depressive-like states. Mice placed in an inescapable transparent tank filled with water their escape related mobility behavior measured. straightforward to conduct reliably it requires minimal specialized equipment. Successful implementation the adherence certain procedural details minimization unwarranted...
A mood stabilizing and antidepressant response to lithium is only found in a subgroup of patients with bipolar disorder depression. Identifying strains mice that manifest differential behavioral responses may assist the identification genomic other biologic factors play role responsiveness. Mouse were tested forced swim test (FST), tail suspension (TST) open-field after acute chronic systemic intracerebroventricular (ICV) treatments. Serum brain levels measured. Three (129S6/SvEvTac,...
Neuromodulatory signaling is poised to serve as a neural mechanism for gain control, acting crucial tuning factor influence neuronal activity by dynamically shaping excitatory and inhibitory fast neurotransmission. The endocannabinoid (eCB) system, the most widely expressed neuromodulatory system in mammalian brain, known filter inputs through retrograde, pre-synaptic action. However, whether eCBs exert retrograde control ultimately facilitate reward-seeking behaviors freely moving mammals...
Abstract Obsessive-compulsive disorder (OCD) is a chronic and severe psychiatric for which effective treatment options are limited. Structural functional neuroimaging studies have consistently implicated the orbitofrontal cortex (OFC) striatum in pathophysiology of disorder. Recent genetic evidence points to involvement components excitatory synapse etiology OCD. However, transcriptional alterations that could link risk known structural abnormalities remain mostly unknown. To assess...
Abstract Medial nucleus accumbens shell (mNAcSh) is a critical brain region for driving motivated behaviors. Despite this well-established role, the underlying reward processing of individual neurons, circuits and cell-types within mNAcSh remains largely unknown. Here, we leverage deep 2-photon calcium imaging through endoscopic lenses to record spiny projection neuron (SPN) ensemble responses rewards different concentrations reward-predictive cues across cue-reward learning. Reward were...
Opinion Statement Clinical and preclinical studies have uncovered substantial evidence that dysfunction in cortico-striatal-thalamo-cortical (CSTC) loops central to the selection of action strategies may underlie obsessive compulsive disorder (OCD) symptoms. In human OCD, data suggest balance between selections habitual versus goal-directed is disrupted, with concomitant hyperactivation CSTC regions associated these strategies. Preclinical lesion inactivation homologous rodents shed light on...
Abstract Although much is known about how corticostriatal circuits mediate behavioral selection, most previous work has been conducted in highly trained animals engaged instrumental tasks. Understanding selection and initiation a naturalistic setting critical to understanding the brain chooses executes behavior unconstrained situations. Central striatum (CS), an understudied region that lies middle of motor-limbic topography, well-poised play important role these processes since its main...
Abstract Compulsive behaviors are a hallmark symptom of obsessive compulsive disorder (OCD). Striatal hyperactivity has been linked to behavior generation in correlative studies humans and causal rodents. However, the contribution two distinct striatal output populations treatment is unknown. These direct indirect pathway-projecting spiny projection neurons (SPNs) have classically thought promote or suppress actions, respectively, leading long-held hypothesis that increased relative pathway...
Summary The basolateral amygdala (BLA) is an evolutionarily conserved brain region, well known for valence processing. Despite this central role, the relationship between activity of BLA neuronal ensembles in response to appetitive and aversive stimuli subsequent expression valence-specific behavior has remained elusive. Here we leverage 2-photon calcium imaging combined with single cell holographic photostimulation through endoscopic lens implanted deep demonstrate a direct causal role...