The effective healing of a bone defect is dependent on the careful coordination inflammatory and bone-forming cells. In current work, pro-inflammatory M1 anti-inflammatory M2 macrophages were co-cultured with primary murine mesenchymal stem cells (BMSCs), in vitro, to establish cross-talk among polarized BMSCs, as well their effects osteogenesis. Meanwhile, influence osteogenesis BMSCs through paracrine forms such exosomes. We focused whether exosomes promote osteogenic differentiation....

Abstract Repairing damage in the craniofacial skeleton is challenging. Craniofacial bones require intramembranous ossification to generate tissue-engineered bone grafts via angiogenesis and osteogenesis. Here, we designed a mineralized collagen delivery system for BMP-2 vascular endothelial growth factor (VEGF) implantation into animal models of mandibular defects. BMP-2/VEGF were mixed with which was implanted rabbit mandibular. Animals divided (i) controls no factors; (ii) alone; or (iii)...

Biomaterials regulate macrophages and promote regeneration function, which is a new hot pot in tissue engineering regenerative medicine. The research based on macrophage materials biology has appeared happy future, but related regulating promoting still its infancy. surface roughness of biomaterials one the important factors affecting behavior. Previous study also found that many polarization, not including mineralized collagen (MC). In this study, we designed fabricated MC with different...

The design of a novel interpenetrating network hydrogel inspired by the microscopic architecture natural cartilage based on supramolecular sodium alginate (SA) nanofibril is reported in this paper. mechanical strength and toughness poly(vinyl alcohol) (PVA) were significantly improved after being incorporated with network. multiple hydrogen bonds between PVA chains fibers provided an efficient energy dissipation, thus leading to significant increase PVA/SA/NaCl hydrogel. demonstrated...

Abstract In the bone immune microenvironment, cells can regulate osteoblasts through a complex communication network. Macrophages play central role in mediating osteogenesis, exosomes derived from them have osteogenic regulation and be used as carriers tissue engineering. However, there are problems with exosomal therapy alone, such poor targeting, content of loaded molecules cannot reach therapeutic concentration. this study, macrophage-derived modified miR-365-2-5p were developed to...

Ti6Al4V artificial implants are increasingly demanded for addressing human dysfunction caused by an aging population and major diseases. However, they restricted due to the release of vanadium aluminum ions in process corrosion wear. This work is aimed provide a protective film implants, then, Si-incorporated diamond-like carbon (Si-DLC) Si- N-incorporated DLC (SiN-DLC) were deposited on surface plasma-enhanced chemical vapor deposition. Results suggest that thickness as-deposited...

Abstract Immune response is an important factor in determining the fate of bone replacement materials, which macrophages play role. It a new idea to design biomaterials with immunomodulatory function reduce inflammation and promote integration by regulating polarization. In this work, properties CaP Zn-Mn-Li alloys specific mechanism action were investigated. We found that Zn0.8Mn0.1Li alloy promoted polarization toward M2 reduced inflammation, could effectively upregulate...

Bone immune responses based on macrophages are critical in the osteogenesis of bone abnormalities. In general, M2 macrophage facilitate promotion osteogenesis, as well, M1 play an important role early healing, confirmed by previous studies. However, it is not clear how involved response. MiR-21a-5p a highly expressed microRNA contrast to M2. Therefore, current work sought ascertain influence healing via exosomal miR-21a-5p and probable mechanism. We discovered that injecting exosomes...

Creating both strong and tough hydrogel materials have been a challenging task, due to their nature of generally mutual exclusion. Interestingly, natural such as bone shells usually offer an unprecedented combination strength toughness, which would provide inspiration for novel materials. Herein, the design cartilage is mimicked with collagen fibers incorporated into extracellular matrix, fabric‐reinforced composite hydrogels are prepared. The poly (vinyl alcohol) (PVA) exhibits significant...

Abstract Hydration lubrication plays an important role including the cartilage of organism. Here, polystyrene nanospheres modified with a hydrophilic polymer through subsurface‐initiated grafting as biolubricating additive is proposed. The material provides good tribological performance when subjected to high load. Compared surfaced‐initiated brushes, subsurface initiation can form much thicker hydration layers obtain lower friction coefficient and better mechanical properties for...

Zn-based alloys are considered as new kind of potential biodegradable implanted biomaterials recently. The difficulty metal and bone tissue integration seriously affects the applications scaffolds in tissue-related fields. Herein, we self-designed Zn0.8Mn Zn0.8Mn0.1Li CaP coated alloys, then evaluated degradation property cytocompatibility. results demonstrated that had profoundly modified cytocompatibility, but particularly adverse effects on surface morphology osteoblasts. furtherly showed...

Rib segment, as one of the most widely used autologous boneresources for bone repair, is commonly isolated with an empty left in defect. Although defective rib repair thought to be unnecessary traditionally, it's vital importance actually promote regeneration patients better postoperative recovery and higher life quality. Comparative investigations on rabbit without graft were reported this article. A segmental defect was performed 8th 4-month-old male New Zealand rabbits. The mineralized...

It is a new hot pot in tissue engineering and regenerative medicine to study the effects of physicochemical properties implanted biomaterials on regulating macrophage polarization promote bone regeneration. In this study, we designed fabricated mineralized collagen (MC) with different microporous structures via vitro biomimetic mineralization method. The structures, mechanical properties, shore hardness water contact angle measurements were tested. Live/dead cell staining, CCK-8 assay,...

Mineralized collagen (MC) is a biomaterial that commonly used in the treatment of bone defects. However, inflammatory response after implantation recurrent problem requires urgent attention. Our previous studies on MC-macrophage interactions were descriptive but we did not perform an in-depth analysis genetic level to investigate underlying mechanisms. In this study, cultured RAW264.7 cells MC or and examined proliferation macrophages by Cell Counting Kit-8 assay. We sequenced RNA discover...

Abstract Nowadays, the whole greenhouse effect is becoming more and serious. It common mission of mankind to curb global warming. As world’s second largest gas (GHG), warming potential caused by methane (CH 4 ) not negligible, so it imperative calculate reduce its emissions. For lack CH accounting methodology in China’s petrochemical chemical industries, this paper proposes a based on enterprise’s boundary. Emission sources include emissions from fuel combustion., flare combustion, escape,...