A5100398799- Extracellular vesicles in disease
- MicroRNA in disease regulation
- Cancer-related molecular mechanisms research
- Immune Response and Inflammation
- RNA modifications and cancer
- Inflammasome and immune disorders
- Immunotherapy and Immune Responses
- Circular RNAs in diseases
- CRISPR and Genetic Engineering
- Viral Infections and Vectors
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- RNA Interference and Gene Delivery
- RNA and protein synthesis mechanisms
- Connexins and lens biology
- Angiogenesis and VEGF in Cancer
- Cancer, Hypoxia, and Metabolism
- CAR-T cell therapy research
- Advanced Glycation End Products research
- Nerve injury and regeneration
- Alkaloids: synthesis and pharmacology
- Cancer Genomics and Diagnostics
- Alzheimer's disease research and treatments
- Allelopathy and phytotoxic interactions
- Epigenetics and DNA Methylation
- Cancer, Stress, Anesthesia, and Immune Response
Jilin University
2016-2024
Hebei Medical University
2013-2024
Liaocheng People's Hospital
2022-2024
Wuhan University of Technology
2022-2024
Lanzhou University
2021-2024
Second People Hospital of Hunan
2024
Hunan University of Traditional Chinese Medicine
2024
Shandong First Medical University
2023-2024
University of South China
2011-2023
Nantong University
2023
Clinical work and research on diabetic wound repair remain challenging globally. Although various conventional dressings have been continuously developed, the efficacy is unsatisfactory. The effect of drug delivery limited by depth penetration. sustained release biomolecules from biological a promising treatment approach to healing. An assortment cell-derived exosomes (exos) proved be instrumental in tissue regeneration, researchers are dedicated developing carriers with unique properties....
Recent studies demonstrated that metformin exerts anti-neoplastic effect in a spectrum of malignancies. However, the mechanism whereby affects various cancers, including gastric cancer, is poorly elucidated. Considering apoptosis plays critical role tumorigenesis, we, present study, investigated vitro apoptotic on human cancer cell and underlying mechanism. Three differently-differentiated lines, MKN-28, SGC-7901 BGC-823, along with one noncancerous line GES-1 were used. We found treatment...
The effective healing of a bone defect is dependent on the careful coordination inflammatory and bone-forming cells. In current work, pro-inflammatory M1 anti-inflammatory M2 macrophages were co-cultured with primary murine mesenchymal stem cells (BMSCs), in vitro, to establish cross-talk among polarized BMSCs, as well their effects osteogenesis. Meanwhile, influence osteogenesis BMSCs through paracrine forms such exosomes. We focused whether exosomes promote osteogenic differentiation....
Background: The purpose of this study was to prepare neutrophil membrane engineered Panax ginseng root derived exosomes (N-exo) and investigate the effects N-exo miRNA 182-5p (N-exo-miRNA 182-5p) on acute lung injury (ALI) in sepsis. Methods: were separated by differential centrifugation. Neutrophil engineering performed exo obtain N-exo. miRNA182-5p transmitted into electroporation technology N-exo-miRNA 182-5p. LPS used establish an vivo vitro model sepsis evaluate anti-inflammatory effect...
Paeoniflorin, the main active component of peony plant, exerts various pharmacological effects. Recently, research on effect paeoniflorin nervous system has gained more attention. The aim present study was to determine whether a protective that improves Alzheimer's disease (AD) via inflammation and apoptosis in cerebral cortex transgenic mouse model AD. Transgenic mice were used construct AD treated with paeoniflorin. Morris water maze test analyze cognitive function mice. protein expression...
General control non-depressible 5 (GCN5) is a crucial catalytic component of transcriptional regulatory complex that plays important roles in cellular functions from cell cycle regulation to DNA damage repair. Although GCN5 has recently been implicated certain oncogenic roles, its role liver cancer progression remains vague.In this study, we report was overexpressed 17 (54.8 %) 31 human hepatocellular carcinoma (HCC) specimens. Down-regulation inhibited HCC proliferation and xenograft tumor...
Urokinase plasminogen activator receptor (uPAR), a member of the lymphocyte antigen 6 protein superfamily, is overexpressed in different types cancers and plays an important role tumorigenesis development. In this study, we successfully targeted uPAR by CRISPR/Cas9 system two human cancer cell lines with individual sgRNAs. Knockout inhibited proliferation, migration invasion. Furthermore, knockout decreases resistance to 5-FU, cisplatin, docetaxel, doxorubicin these cells. Although there are...
Abstract In the bone immune microenvironment, cells can regulate osteoblasts through a complex communication network. Macrophages play central role in mediating osteogenesis, exosomes derived from them have osteogenic regulation and be used as carriers tissue engineering. However, there are problems with exosomal therapy alone, such poor targeting, content of loaded molecules cannot reach therapeutic concentration. this study, macrophage-derived modified miR-365-2-5p were developed to...
Abstract BACKGROUND Carboxyl/cholinesterases (CCEs) are thought to play a pivotal role in the degradation of sex pheromones and plant‐derived odorants insects, but their exact biochemistry physiological functions remain unclear. RESULTS In this study, two paralogous antennae‐enriched CCEs from Plutella xylostella (PxylCCE16a 16c) were identified functionally characterized. High‐purity protein preparations active recombinant PxylCCE16a 16c have been obtained Sf 9 insect cells by Ni 2+...
Aggressive tumor cells can mimic embryonic vasculogenic networks and form mimicry (VM). Preliminary studies demonstrated that hypoxia promote VM formation; however, the underlying mechanism remains unclear. The present study aimed to investigate role of Twist1‑Bmi1 connection in hypoxia‑induced formation mechanism. In vitro experiments, western blot analysis upregulated expression Twist1, Bmi1, epithelial‑mesenchymal transition (EMT) markers, stem cell markers VM‑associated markers. 3D...
To study the immune-inflammatory response and signaling mechanism of macrophages to purified Enterococcus faecalis (E. faecalis) lipoteichoic acid (LTA), intact LTA was obtained from an E. clinical strain P25RC using butanol method hydrophobic interaction chromatography purification. The fractions containing were determined phosphate detection. Contaminations with lipopolysaccharide proteins excluded Limulus amoebocyte lysate assay sodium dodecyl sulfate‑polyacrylamide gel electrophoresis,...
Resveratrol has also been approved for use in enhancing plant disease resistance and reducing pesticide use. A number of studies have shown that the crops treated with resveratrol is markedly improved. The aim present study was to examine protective effect against alcohol‑induced neurodegeneration occurred its association AMP‑activated protein kinase (AMPK)/sirtuin 1 (SIRT1)/p38 rats humans. ELISA, caspase‑3 activity western blot analyses were employed study. Sprague‑Dawley human...
Abstract Background Colorectal cancer (CRC) is a leading and major cause of death. Lipopolysaccharide (LPS), an abundant component in gut microbiome, involved CRC progression metastasis, potentially through regulating the miRNA composition CRC-derived exosomes. In this study, we aimed to identify species exosome which regulates after LPS stimulation. Results Firstly, discovered shift profile Among differentially expressed miRNAs, identified miR-200c-3p as potential key regulator metastasis....
Boronic ester hydrogels are widely used in biomedicine for their stimulus responsiveness to multiple disease-related triggers. However, applicability is limited by its low hydrolytic stability. Here, we created the highest hydrolytically stable boronic cross-linked network yet documented─novel diisopropanolamine (DIPAB) hydrogel. The systematic study from a molecular level three-dimensional hydrogel demonstrates that DIPAB has significantly greater stability and dynamic exchange activation...
A carbonic anhydrase mimic converting CO<sub>2</sub> to acid, deprotonated under highly basic conditions, and being converted a carbamate upon reaction with monoethanolamine, solvent reported for carbon capture reactions.
The tumor immune microenvironment significantly affects occurrence, progression, and prognosis, but its impact on the prognosis of low-grade glioma (LGG) patients with epilepsy has not been reported. Hence, purpose this study is to explore effect LGG epilepsy.The data derived from TCGA database. level cell infiltration proportion 22 cells were evaluated by ESTIMATE CIBERSORT algorithms, respectively. Cox LASSO regression analysis was adopted determine DEGs, further established clustering...
Bone immune responses based on macrophages are critical in the osteogenesis of bone abnormalities. In general, M2 macrophage facilitate promotion osteogenesis, as well, M1 play an important role early healing, confirmed by previous studies. However, it is not clear how involved response. MiR-21a-5p a highly expressed microRNA contrast to M2. Therefore, current work sought ascertain influence healing via exosomal miR-21a-5p and probable mechanism. We discovered that injecting exosomes...
Efficient catalytic asymmetric synthesis of 1,1-disubstituted tetrahydro-β-carbolines has been achieved via alkylation 1-cyanotetrahydro-β-carbolines using a binaphthyl-modified N-spiro-type chiral phase-transfer catalyst. This is valuable example hitherto difficult highly enantioselective alkylations at α-carbon the cyano group under conditions.
Interspecies somatic cell nuclear transfer (iSCNT) has been regarded as a potential alternative for rescuing highly endangered species and can be used model studying nuclear–cytoplasmic interactions. However, iSCNT embryos often fail to produce viable offspring. The alterations in normal molecular mechanisms contributing extremely poor development are the most part unknown. Przewalski's gazelle–bovine (PBNT) were produced by transferring gazelle fibroblast nuclei into enucleated bovine...
Deep eutectic solvents (DESs) are increasingly receiving interest as a new type of green and sustainable alternative to hazardous organic solvents. In this work, novel DES based on levulinic acid (La) 1,4-butanediol (Buta) an extraction media was developed for extracting the bioactive alkaloid rutaecarpine from unripe fruits Tetradium ruticarpum. 24 different DESs consisting choline chloride, betaine, sugar alcohols, acids, amides, sugars were prepared tailored test their efficiency. After...