A5028192803- Cancer Cells and Metastasis
- Angiogenesis and VEGF in Cancer
- Microtubule and mitosis dynamics
- Cancer-related Molecular Pathways
- Cancer, Hypoxia, and Metabolism
- Epigenetics and DNA Methylation
- RNA Research and Splicing
- Cancer-related molecular mechanisms research
- Cancer Genomics and Diagnostics
- interferon and immune responses
- HER2/EGFR in Cancer Research
- RNA regulation and disease
- Cancer Mechanisms and Therapy
- Microbial metabolism and enzyme function
- RNA and protein synthesis mechanisms
- Ferroptosis and cancer prognosis
- Radiopharmaceutical Chemistry and Applications
- DNA Repair Mechanisms
- Cancer-related gene regulation
- Cancer, Lipids, and Metabolism
- RNA modifications and cancer
- Circular RNAs in diseases
- Inflammatory mediators and NSAID effects
- Biomarkers in Disease Mechanisms
- Molecular Biology Techniques and Applications
Tianjin Medical University General Hospital
2012-2024
Tianjin Medical University
2014-2024
Hebei Medical University
2014-2016
Fourth Hospital of Hebei Medical University
2014-2016
Tianjin Medical University Cancer Institute and Hospital
2006-2011
Mayo Clinic in Florida
2010
Sanford Burnham Prebys Medical Discovery Institute
2010
Van Andel Institute
2010
The incidence and mortality rates of hepatocellular carcinoma (HCC) have steadily increased in recent years. A hypoxic microenvironment is one the most important characteristics solid tumors which has been shown to promote tumor metastasis, epithelial-mesenchymal transition angiogenesis. Epithelial-mesenchymal vasculogenic mimicry regarded as crucial contributing factors cancer progression. HIF-1α functions a master transcriptional regulator adaptive response hypoxia. Lysyl oxidases like 2...
Abstract Prostaglandin E receptor 3 (PTGER3) is involved in a variety of biological processes the human body and closely associated with development progression cancer types. However, role PTGER3 triple‐negative breast (TNBC) remains unclear. In present study, low expression was found to be poor prognosis TNBC patients. plays crucial regulating cell invasion, migration, proliferation. Upregulation weakens epithelial–mesenchymal phenotype promotes ferroptosis both vitro vivo by repressing...
Abstract Vasculogenic mimicry ( VM ) refers to the unique capability of aggressive tumour cells mimic pattern embryonic vasculogenic networks. Epithelial–mesenchymal transition EMT regulator slug have been implicated in invasion and metastasis human hepatocellular carcinoma HCC ). However, relationship between formation is not clear. In study, we demonstrated that expression was associated with cancer stem cell (CSCs) phenotype patients. Importantly, showed statistically correlation...
Vasculogenic mimicry (VM), a newly defined pattern of tumor blood supply, describes the functional plasticity aggressive cancer cells that form vascular networks. In our previous study, breast stem (CSC) were shown to potentially participate in VM formation. this CSCs presented centrosome amplification (CA) phenotype and ubiquitin-specific protease 44 (USP44) upregulation. USP44 expression contributed establishment bipolar spindles with supernumerary centrosomes by localizing at...
Abstract Vasculogenic mimicry (VM) refers to the condition in which tumour cells mimic endothelial form extracellular matrix‐rich tubular channels. VM is more extensive aggressive tumours. The human epidermal growth factor receptor 2 (HER2) gene amplified 20–30% of breast cancers and has been implicated mediating metastasis. However, thus far, there have no data on role HER2 formation. Immunohistochemical histochemical double‐staining methods were performed display cancer specimens....
To evaluate the prognostic value of OCT4 expression and vasculogenic mimicry (VM) in human breast cancer, we examined VM formation using immunohistochemistry CD31/PAS (periodic acid-schiff) double staining on 90 cancer specimens. All patients were followed up for five-149 months following surgery. Survival curves generated Kaplan-Meier method. Multivariate analysis was performed Cox regression model to assess values. Results showed positive correlation between (p<0.05). Both also positively...
Treatment of triple-negative breast cancer (TNBC) has been challenging, and paclitaxel resistance is one the major obstacles to better prognosis. Deregulation alternative splicing (AS) may contribute tumor progression chemotherapy resistance. Human AS factor TRA2 two separate gene paralogs encoding TRA2A TRA2B proteins. associated with cell survival therapeutic sensitivity. However, individual role in not reported. Here we report that facilitates proliferation migration invasion TNBC cells....
Vasculogenic mimicry (VM) refers to the unique capability of aggressive tumor cells mimic pattern embryonic vasculogenic networks. Cancer stem (CSC) represent a subpopulation endowed with capacity for self‐renewal and multilineage differentiation. Previous studies have indicated that CSC may participate in formation VM. With advance high‐resolution microarrays massively parallel sequencing technology, long noncoding RNAs (lncRNAs) are suggested play critical role tumorigenesis and,...
Vasculogenic mimicry (VM) is a nonangiogenesis-dependent pathway that promotes tumor growth and disease progression. Nodal signaling has several vital roles in both embryo development cancer However, the effects of on VM formation breast its underlying mechanisms are ill-defined. We analyzed relationship between one hundred human cases results showed expression was significantly correlated with formation, metastasis, differentiation grade, TNM stage poor prognosis. Furthermore, up-regulation...
Abstract HnRNPM is an essential splicing factor and its expression closely correlated with invasion metastasis of tumor cells. The CD44 cell adhesion molecule aberrantly expressed in many breast tumors splice variants have been implicated specific oncogenic signaling pathways. To investigate the clinical significance biological function hnRNPM, immunohistochemistry, quantitative, semiquantitative polymerase chain reaction, lentiviral transfection system transwell assays were performed. We...
Abstract Lysyl oxidase‐like 2 ( LOXL 2) has shown to promote metastasis and poor prognosis in hepatocellular carcinoma HCC ). Also, we have previously reported that vasculogenic mimicry VM ) is associated with invasion, survival patients. In the present study, investigated molecular function of . We used immunohistochemical CD 31/periodic acid‐Schiff double staining detect relationship between formation. performed gain loss studies analysed migratory, invasion tube formation cell lines. both...
Centrosomal abnormalities have been found in various cancer types. We sought to determine whether centrosomal dysfunctions occur the atypical ductal hyperplasia (ADH)-carcinoma sequence of breast cancer. As alpha and gamma-tubulins are structural components centrosomes, we performed real time quantitative polymerase chain reaction (qPCR), situ hybridization (ISH) immunnohistochemistry (IHC) DNA copy levels, messenger RNA (mRNA) expression, protein expression respectively. Gamma-tubulin...
Abstract When cells encounter substantial DNA damage, critical cell cycle events are halted while repair mechanisms activated to restore genome integrity. Genomic integrity also depends on proper assembly and function of the bipolar mitotic spindle, which is required for equal chromosome segregation. Failure execute either these processes leads genomic instability, aging, cancer. Here, we show that following damage in breast cancer line MCF-7, centrosome protein centrin2 moves from cytoplasm...
Aggressive tumor cells can mimic embryonic vasculogenic networks and form mimicry (VM). Preliminary studies demonstrated that hypoxia promote VM formation; however, the underlying mechanism remains unclear. The present study aimed to investigate role of Twist1‑Bmi1 connection in hypoxia‑induced formation mechanism. In vitro experiments, western blot analysis upregulated expression Twist1, Bmi1, epithelial‑mesenchymal transition (EMT) markers, stem cell markers VM‑associated markers. 3D...
Abstract Matrix metalloproteinases ( MMP s) have critical functions in tumour vasculogenic mimicry VM ). This study explored the mechanisms underlying ‐13 and ‐2 regulation of formation large cell lung cancer LCLC In our study, laminin5 (Ln‐5) fragments cleaved by promoted tubular structure lines H460 H661 three‐dimensional (3D) cultures. Transient up‐regulation or treatment with recombinant protein abrogated cells 3D culture. Treated Ln‐5 stimulated EGFR F‐actin expression. decreased...
Transcription factor Slug plays an important role in the tumor invasion and metastasis of human hepatocellular carcinoma (HCC). This study aimed to explore mechanism involved promotion HCC progression by Slug. In precent study, we demonstrated that expression was significantly associated with shorter survival time patients. Using ChIP-on-chip microarray analysis, identified molecular profile downstream targets cells overexpression. The Wnt, Notch Hedgehog pathways were promote pluripotency...
Wang S, Li W, Lv Y, Liu Z, Zhang J, T & Niu Y(2011) Histopathology59, 631–642 Abnormal expression of Nek2 and β-catenin in breast carcinoma: clinicopathological correlations Aims: NIMA-related kinase 2 (Nek2) are important centrosome regulatory factors. The aim this study was to detect the possible disparity their among normal tissue, invasive ductal carcinoma (IDC), concomitant situ (DCIS), pure DCIS, explore its correlation with Methods results: We used immunohistochemistry protein cancer...
Basal-like breast cancer (BLBC) is resistant to endocrinotherapy and targeted therapy new molecular therapies are needed for BLBC. In this study, we evaluated the role of DUSP1 DUSP5, negative regulators mitogen-activated protein kinase pathway, in aggressiveness MDA-MB-231 cells were given paclitaxel (PTX) treatment subsequently PTX cell clones established. Microarray analysis, real-time quantitative reverse transcription PCR (qRT-PCR), online analysis large cohorts patients performed. The...
Pulmonary sarcomatoid carcinomas (PSCs) are a rare subtype of non‑small‑cell lung cancer and typically biphasic neoplasms. No effective treatment for PSCs is currently available in clinical practice. The expression the epithelial‑mesenchymal transition (EMT) transcription factors, Twist1, Slug Snail, as well EMT phenotype vasculogenic mimicry (VM) were analysed 41 PSC 79 pulmonary squamous carcinoma (PSCC) samples. Compared with PSCCs, exhibited an VM, they also increased Slug, Snail VM...
Vasculogenic mimicry (VM) is a unique blood supply pattern in malignant tumors that closely associated with metastasis and poor prognosis. The Hippo signaling effector TAZ upregulated several cancers, promoting cancer proliferation metastasis. This study aimed to identify the function of its regulatory mechanism VM gastric (GC).The expression TEAD4 their correlations overall survival VM-related markers were analyzed 228 cases GC. interaction epithelial-mesenchymal transition (EMT)...