Abstract Aims The dysregulation of TGF‐β signaling is a crucial pathophysiological process in tumorigenesis and progression. LncRNAs have diverse biological functions are significant participants the regulation tumor pathways. However, clinical value lncRNAs related to glioma currently unclear. Methods Data on glioma's RNA‐seq transcriptome, somatic mutation, DNA methylation data, clinicopathological information were derived from CGGA TCGA databases. A prognostic lncRNA signature was...

Background/Aims: Hepatocellular carcinoma (HCC) is one of the most deadly diseases; metastasis and recurrence are important factors that affect therapy HCC chronically. Until now, prognosis for had not improved. Recently, several proteins related to invasion were identified, but effective markers still remain be elucidated. Methods: In this study, comparative proteomics was used study differentially expressed in two cell lines MHCC97L HCCLM9, which have low high metastatic potentials,...

Drought-resistant varieties are an important way to address the conflict between wheat's high water demand and scarcity of resources in North China Plain (NCP). Drought stress impacts many morphological physiological indicators winter wheat. To increase effectiveness breeding drought-tolerant varieties, choosing indices that can accurately indicate a variety's drought resistance is advantageous.From 2019 2021, 16 representative wheat cultivars were cultivated field, 24 traits, including...

Establishing crop cultivars with strong tolerance to P and N deprivation, high salinity, drought is an effective way improve yield promote sustainable agriculture worldwide. A vacuolar H+-pyrophosphatase (V-H+-PPase) gene in wheat (TaVP) was functionally characterized this study. TaVP cDNA 2586-bp long encodes a 775-amino-acid polypeptide that contains 10 conserved membrane-spanning domains. Transcription of upregulated by inorganic phosphate (Pi) drought. Transgene analysis revealed...

Agriculture is a labor-intensive industry. However, with the demographic shift toward an aging population, agriculture increasingly confronted labor shortage. The technology for autonomous operation of agricultural equipment in large fields can improve productivity and reduce intensity, which help alleviate impact population on agriculture. Nevertheless, significant challenges persist practical application this technology, particularly concerning adaptability, operational precision,...

Abstract Verticillium wilt diseases caused by the soil‐borne fungus dahliae result in devastating yield losses many economically important crops annually. Here, we identified a novel ethylene‐inducing xylanase (EIX)‐like protein, VdEIX3, from V. , which exhibits immunity‐inducing activity Nicotiana benthamiana . In vitro ‐purified VdEIX3 can induce strong oxidative burst, activate expression of defense‐related genes, and increase resistance against oomycete fungal pathogens N. orthologs...

Summary Receptor‐like cytoplasmic kinase subfamily VII (RLCK‐VII) proteins are the central immune kinases in plant pattern‐recognition receptor (PRR) complexes, and they orchestrate a complex array of defense responses against bacterial fungal pathogens. However, role RLCK‐VII plant–oomycete pathogen interactions has not been established. Phytophthora capsici is notorious oomycete that infects many agriculturally important vegetables. Here, we report identification RXLR25, an RXLR effector...

Phytophthora capsici is one of the most harmful pathogens in agriculture, which threatens safe production multiple crops and causes serious economic losses worldwide. Here, we identified a P. expansin-like protein, PcEXLX1, by liquid chromatography-tandem mass spectrometry from Nicotiana benthamiana apoplastic fluid infected with capsici. Clustered regularly interspaced short palindromic repeats/crispr associated protein 9 (CRISPR/Cas9)-mediated PcEXLX1 knockout mutants exhibited...

Maize grain yield is drastically reduced by heat stress (HTS) during anthesis and early filling. However, the mechanism of HTS in reproductive organs kernel numbers remains poorly understood. From 2018 to 2020, two maize varieties (ND372, tolerant; XY335, sensitive) temperature regimens (HTS, stress; CK, natural control) were evaluated, resulting four treatments (372CK, 372HTS, 335CK, 335HTS). was applied from nine-leaf stage (V9) stage. Various morphological traits physiological activities...

Abstract Background Oral prednisone has been recognized as the first-line therapy for treatment of ocular myasthenia gravis (OMG). However, its long-term use is complicated by numerous adverse effects and ineffective some OMG patients in reaching remission. This study aimed to evaluate effectiveness safety intravenous methylprednisolone (IVMP) tacrolimus monotherapy with unsatisfactory responses conventional therapy. Methods We retrospectively reviewed 57 who had not achieved satisfactory...

The tumor immune microenvironment significantly affects occurrence, progression, and prognosis, but its impact on the prognosis of low-grade glioma (LGG) patients with epilepsy has not been reported. Hence, purpose this study is to explore effect LGG epilepsy.The data derived from TCGA database. level cell infiltration proportion 22 cells were evaluated by ESTIMATE CIBERSORT algorithms, respectively. Cox LASSO regression analysis was adopted determine DEGs, further established clustering...

Abstract Plant cell-surface leucine-rich repeat receptor-like kinases (LRR-RLKs) and proteins (LRR-RLPs) form dynamic complexes to receive a variety of extracellular signals. LRR-RLKs are also widespread in oomycete pathogens, whereas it remains enigmatic whether plant could mediate cell-to-cell communications between pathogen host. Here, we report that an LRR-RLK from the soybean root stem rot Phytophthora sojae , PsRLK6, can activate typical pattern-triggered immunity host nonhost tomato...

BackgroundAutoimmune encephalitis (AE) is a neuroautoimmune disease featured by the presence of antibodies targeting neuronal surface, synaptic, or intracellular antigens. An increasing number articles on its clinical manifestations, treatments, and prognosis have appeared in recent years. The objectives this study were to summarize growing body literature provide an overview hotspots trends AE research using bibliometric analysis.MethodsWe retrieved AE-related published between 1999 2022...

The neuromuscular adverse events of immune checkpoint inhibitor (ICI) treatment include myositis, polymyalgia rheumatica, myocarditis, and myasthenia syndrome. We report a 47-year old female presenting with external ophthalmoplegia, generalized muscle weakness, third-degree atrioventricular block 4 weeks after toripalimab for metastatic thymoma. Creatine kinase was elevated to 25,200 U/l cardiac troponin I 2.796 ng/ml. Autoantibody profiling shows positive anti-ryanodine receptor...

Autosomal recessive polycystic kidney disease (ARPKD) is a severe inherited disorder with an incidence of 1/20 000 live births. Mutations PKHD1 (polycystic and hepatic gene 1) were identified to be responsible for ARPKD. However, the underlying molecular mechanisms remain largely unknown. MicroRNAs (miRNAs) are abundant class small RNAs global effect on expression. Up 30% human protein coding genes may regulated by miRNAs. date, nothing known regarding role miRNAs in . In this study, we...

Plant microRNAs (miRNAs) play important roles in regulating plant growth, development, and responses to abiotic stresses. In this study, 38 miRNAs (TaMIRs) from wheat (Triticum aestivum L.), 36 the miRBase database, two our previous work were characterized subjected an expression pattern analysis under normal conditions a drought stress. A semiquantitative reverse transcriptase polymerase chain reaction (RT-PCR), real-time quantitative PCR (qPCR), small RNA blot analyses revealed that TaMIRs...

Abstract Carbohydrate‐binding module family 1 (CBM1) is a cellulose‐binding domain that almost exclusively found in fungi and oomycetes. CBM1‐containing proteins (CBPs) have diverse architectures play pivotal roles the plant–microbe interaction. However, only few CBPs been functionally investigated. In this study, we identified PcCBP3 an oomycete pathogen, Phytophthora capsici . contains two tandem CBM1 domains its orthologs from other species exhibit diversity including gene loss,...

Background Lymphoplasmapheresis (LPE) is a new therapy developed on the basis of traditional plasma exchange (PE) in combination with leukapheresis. Currently, it remains unclear whether PE and LPE show differences efficacy for severe MG. Methods A retrospective analysis was conducted 198 MG patients, 75 group 123 group, patients’ Myasthenia Gravis Foundation America (MGFA) Clinical Classification Class IV. The treatment outcome change Quantitative Score (QMGS) from baseline to end...

Zinc finger protein (ZFP) genes comprise a large and diverse gene family, are involved in biotic abiotic stress responses plants. In this study, total of 126 ZFP classified into various types wheat were characterized subjected to expression pattern analysis under inorganic phosphate (Pi) deprivation. The their corresponding GenBank numbers obtained from the information 4×44K microarray chip. They confirmed by sequence similarity named based on homologs Brachypodium distachyon or Oriza...

Abstract Due to their sessile nature, plants must respond various environmental assaults in a coordinated manner. The endoplasmic reticulum is central hub for plant responses stresses. We previously showed that Phytophthora utilizes effector PsAvh262-mediated binding immunoglobulin protein (BiP) accumulation suppressing stress-triggered cell death. As BiP partner, Bcl-2-associated athanogene 7 (BAG7) plays crucial role the maintenance of unfolded response, but little known about its...

Abstract Water scarcity is a key constraint to crop production in North China Plain (NCP), which produces the majority of country’s winter wheat ( Triticum aestivum L.). The objective this three-year field study was see whether and when irrigation one-time spring improved grain productivity water use efficiency. Four sets were established at 3-leaf visible stage (L3) L4, L5, L6 stages. When time postponed, spike number, 1000-grain weight, consumption increased progressively, whereas yield,...

Diseases caused by the notorious Phytophthora spp. result in enormous economic losses to crops and forests. Increasing evidence suggests that small open reading frame-encoded polypeptides (SEPs) participate environmental responses of animals, plants, fungi. However, it remains largely unknown whether pathogens produce SEPs. Here, we systematically predicted identified 96 SEP candidates P. capsici. Among them, three may induce stable cell death Nicotiana benthamiana. Phytophthora-specific...