A5069821150- Prostate Cancer Diagnosis and Treatment
- Prostate Cancer Treatment and Research
- Endometrial and Cervical Cancer Treatments
- Advanced Radiotherapy Techniques
- Management of metastatic bone disease
- MRI in cancer diagnosis
- Ovarian cancer diagnosis and treatment
- Urinary Bladder and Prostate Research
- Radiomics and Machine Learning in Medical Imaging
- Radiation Dose and Imaging
- Angiogenesis and VEGF in Cancer
- Urological Disorders and Treatments
- Bladder and Urothelial Cancer Treatments
- Hepatocellular Carcinoma Treatment and Prognosis
- Breast Implant and Reconstruction
- Gastric Cancer Management and Outcomes
- RNA modifications and cancer
- Radiopharmaceutical Chemistry and Applications
- Cancer Diagnosis and Treatment
- Cancer survivorship and care
- Breast Cancer Treatment Studies
- Dispute Resolution and Class Actions
- Urologic and reproductive health conditions
- Advances in Oncology and Radiotherapy
- Genital Health and Disease
Kelowna General Hospital
2016-2025
University of British Columbia
2010-2024
BC Cancer Agency
2010-2021
Université de Sherbrooke
2007-2019
McGill University Health Centre
2016
Interior Health
2015
McGill University
1999-2009
Hôtel-Dieu de Québec
2007-2008
Centre hospitalier universitaire de Québec
2007-2008
Université Laval
2008
ABSTRACT The use of proximity-dependent biotinylation assays coupled to mass spectrometry (PDB-MS) has changed the field protein–protein interaction studies. However, despite recurrent and successful BioID-based interactions screening in mammalian cells, implementation PDB-MS yeast not been effective. Here, we report a simple rapid approach effectively screen for proximal interacting proteins their natural cellular environment by using TurboID, recently described version BirA biotin ligase....
PURPOSE To report clinical and treatment characteristics, remission failure patterns, risk factors for local (LF) from the EMBRACE-I study. MATERIALS AND METHODS was a prospective, observational, multicenter cohort study on magnetic resonance imaging–based image-guided adaptive brachytherapy (MR-IGABT) in locally advanced cervical cancer. Treatment consisted of external beam radiotherapy, concurrent chemotherapy, MR-IGABT. LF defined as progressive or recurrent disease cervix, uterus,...
Purpose/ObjectivesTo compare health-related Quality of Life (QoL) in urinary, bowel and sexual domains after combined external beam radiotherapy (EBRT) either low dose rate (LDR) or high (HDR) prostate brachytherapy (BT).Materials/MethodsEligible men with intermediate high-risk cancer, treated pelvic EBRT BT were randomly assigned to HDR (15 Gy) LDR (110 boost. International Prostate Symptom Score (IPSS), Index Erectile Function (IIEF), Expanded Cancer Composite (EPIC-26) collected at...
The 96-amino acid Vpr protein is the major virion-associated accessory of human immunodeficiency virus type 1 (HIV-1). As not part p55 Gag polyprotein precursor (Pr55gag), its incorporation requires an anchor to associate with assembling viral particles. Although molecular mechanism presently unclear, C-terminal region Pr55gagcorresponding p6 domain appears constitute such essential for protein. In order clarify by which istrans-incorporated into progeny virion particles, we tested whether...
Telomerase is a ribonucleoprotein enzyme complex that adds DNA repeats at the ends of chromosomes. In an effort to establish in vivo heterologous expression system for active human telomerase, we expressed telomerase reverse transcriptase (hTERT) Saccharomyces cerevisiae and affinity-purified protein as fusion with glutathione S-transferase (GST). Addition GST moiety N terminus hTERT did not interfere activity when GST-hTERT was rabbit reticulocyte lysate (RRL) presence RNA (hTR). Active...
Telomere length is maintained in most eukaryotic cells by telomerase. The core components of this ribonucleoprotein (RNP) enzyme include a protein catalytic subunit, composed motifs conserved among reverse transcriptases (RT), and an RNA subunit that contains short template sequence essential for the synthesis telomeric repeats. We developed electrophoretic mobility shift assay using active telomerase partially purified from 293 radiolabeled, vitro-transcribed human (hTR) to investigate...
Synthesis of the pre-mRNA poly(A) tail in nucleus has important consequences on translational activity mature mRNA cytoplasm. In most eukaryotes, nuclear polyadenylation pre-mRNAs is thought to require poly(A)-binding protein (PABP2/PABPN1) for synthesis and ultimate length control. As yet, however, extent association between PABP2 exported remains poorly understood. Here, we used chromatin immunoprecipitation (ChIP) assays show that fission yeast ortholog mammalian (Pab2)...
Introduction: Radium 223 (Ra223) given for six cycles has proven efficacy in clinical trials, but its population-level generalizability not been well-described. The objectives of this study were to describe population-based Ra223 use the abiraterone and enzalutamide era identify factors associated with completion.Methods: All patients at British Columbia Cancer Agency between September 2013 February 2016 identified. Patients who completed <5 vs. ≥5 compared on patient characteristics,...
Defining biochemical failure as nadir + 2 may overestimate cure after radiotherapy. We assessed long-term prostate specific antigen stability low dose rate brachytherapy and predictors of when was slowly rising below the ng/ml threshold.A total 2,339 patients with or intermediate risk cancer received 125iodine from 1998 to 2010 a minimum 3-year followup. In addition, 49.7% 6 months androgen deprivation. Clinical, dosimetric data were retrieved prospective database. Biochemical results...