A5078825725- Liver Disease Diagnosis and Treatment
- Endoplasmic Reticulum Stress and Disease
- Hepatitis B Virus Studies
- Hepatitis C virus research
- Liver Disease and Transplantation
- Lipid metabolism and disorders
- Peroxisome Proliferator-Activated Receptors
- Autophagy in Disease and Therapy
- Sperm and Testicular Function
- Monoclonal and Polyclonal Antibodies Research
- Diet, Metabolism, and Disease
- Urologic and reproductive health conditions
- Hepatitis Viruses Studies and Epidemiology
- Drug-Induced Hepatotoxicity and Protection
- Diet and metabolism studies
- Metabolism and Genetic Disorders
- Testicular diseases and treatments
- Heme Oxygenase-1 and Carbon Monoxide
- Glaucoma and retinal disorders
- Respiratory viral infections research
- Liver physiology and pathology
- Immune cells in cancer
- Parasitic Diseases Research and Treatment
- Cannabis and Cannabinoid Research
- Parasites and Host Interactions
Zhejiang University
2024
Capital Medical University
2012-2023
Beijing YouAn Hospital
2006-2023
Shanghai Municipal Center For Disease Control Prevention
2023
Ningbo Medical Center Lihuili Hospital
2017-2021
Ningbo University
2013
Children's Hospital of Zhejiang University
2008-2009
Huazhong University of Science and Technology
2006
Introduction: To investigate the effect of different levels early intraocular pressure (IOP) on long-term outcomes patients with primary open-angle glaucoma (POAG) who were treated trabeculectomy. Methods: This was a retrospective cohort study, total 74 (90 eyes) trabeculectomy surgery at single center from 2021 to 2022. Based IOP 1 day after surgery, they divided into high group (≥ 15mmHg) and low (< 15mmHg). The Student’s t-test chi-square test used compare differences between two...
Abstract Acute kidney injury (AKI) has become a common disorder with high risk of morbidity and mortality, which remains major medical problem without reliable effective therapeutic intervention. Apoptosis‐stimulating protein two p53 (ASPP2) is proapoptotic member that belongs to binding family, plays key role in regulating apoptosis cell growth. However, the ASPP2 AKI not been reported. To explore progression AKI, we prepared an mouse model induced by ischaemia reperfusion (I/R) wild‐type...
Although endoplasmic reticulum (ER) stress is critical in various liver diseases, its role acute-on-chronic failure (AoCLF) caused by acute exacerbation of chronic hepatitis B (CHB) still elusive. This study aimed to analyse ER responses the progression HBV-related AoCLF. Normal tissues (n = 10), CHB 12) and patients with AoCLF 19) were used. Electron microscopy ultrastructure was carried out on specimens. The gene protein expression levels stress-related genes measured. We further analysed...
Background Acute-on-Chronic Liver Failure (ACLF) patients experience systemic inflammation as well immune dysfunction and exhaustion. The phenotype functionality of monocyte-derived dendritic cells in ACLF with different clinical parameters have not been elucidated. Methods This study included 37 cases ACLF, 20 Chronic Hepatitis B (CHB) patients, 12 healthy controls. Demographic laboratory were collected from the enrolled patients. Peripheral blood samples obtained participants....
To determine the clinical value of hepatic fibrosis parameters and serum ferritin (SF) in obese children with nonalcoholic fatty liver disease.One hundred one aged 6-15 years 30 healthy 9-14 were enrolled study. Body mass index (BMI), waist circumference (WC), alanine aminotransferase (ALT), aspartate (AST), including hyaluronic acid (HA), laminin (LN), type III procollagen (PCIII) IV collagen (CIV), B-ultrasonography assessed. All subjects divided into 4 subgroups: simple (SOC), (SNAFL),...
Abstract The metabolic response to dietary fat intake and its relation with syndrome (MetS) remains unclear. Here, we identified related signature metabolites characterized their associations MetS prevalence. We enrolled 236 participants from the Precision Nutrition Food Safety for Dietary Prevention of Chronic Disease study in China. Nontargeted metabolomics was conducted investigate plasma metabolome. were assessed using elastic net regression a 10‐fold cross‐validation. multi‐metabolite...
Objective To analyze the role of cysteinyl aspartate specific proteinase-1 (caspase-1) in a mouse model D-galactosamine (D-GalN) and lipopolysaccharide (LPS) induced acute liver failure (ALF) to study possible mechanism. Methods C57BL/6 mice were randomly divided into four groups including control group, Z-WEHD-FMK (caspase-1 inhibitor) treatment ALF group Z-WEHD-FMK-treated group. The was established by intraperitoneally injecting with D-GalN (450 mg/kg) LPS (10 μg/kg). damages...
Objective: To investigate the role of glycogen synthase kinase 3β (GSK3β) and peroxisome proliferator-activated receptor alpha (PPARα) signaling pathway in acute liver failure related mechanisms a mouse model induced by D-galactosamine/lipopolysaccharide (D-GalN/LPS). Methods: C57BL/6 mice were given intraperitoneal injection D-GalN/LPS to establish failure. SB216763 was used inhibit activity GSK3β PPARα siRNA expression PPARα. Western blotting measure protein. The changes pathology observed...
Objective: To investigate the effect of calcium-independent phospholipase A(2) (iPLA(2)) inhibitor in reducing hepatocyte lipoapoptosis and improving insulin resistance. Methods: A total 28 male Sprague-Dawley rats were randomly divided into following three groups: 12 group I (normal control group) given normal diet for 18 weeks; 8 II (non-alcoholic fatty liver disease model high-fat III (iPLA(2) weeks intraperitoneal injection iPLA(2) bromoenol lactone 150 μg/kg once every other day since...
Objective To investigate the changes of nuclear factor-κ gene binding (NF-κB) expression in and apoptosis spermatogenic epithelial cells restored testis after torsion analyze relationship between them. Methods Sixteen male SD rats underwent left clockwise at an angle 720° for 2 hours then was to original position fixed. Then 16 were randomly divided into equal group: Group Ⅰ which salicylazosulfapyridium (SASP) suspension infused intra-gastrically 5 h operation once a day 4 times, Ⅱ normal...
To investigate the protective mechanism of endoplasmic reticulum stress (ERS) inhibition against inflammation-induced acute liver injury using a mouse model.Marrow-derived stem cells were isolated from femur and used to derive macrophages for analysis in experimental inflammation conditions, established by exposure LPS consequent activation TLR4. Tunicamycin, an ERS chemical inducer, was applied interfere model condition.Affect on inflammation-related factor MAPK detected western blot,...
Objective To investigate the effects of peroxisome proliferator-activated receptor α (PPARα) on macrophage-mediated inflammatory responses with interference lipopolysaccharide and possible mechanism. Methods The bone marrow stem cells were isolated from femora mice. The granulocyte-macrophage colony stimulating factor (GM-CSF) was used to stimulate in vitro differentiation into primary macrophages. An model cultured expressing cytokines established by treating macrophages (LPS). A...
In order to detect Hepatitis C virus NS3 antigen (HCAg-NS3) in human serum, we developed a magnetic microparticle (MMP) based immunoassay by using biotin-streptavidin amplification, MMP combination with immunoassay. Results showed that the HCV monoclonal antibody had high immobility rate (87.1%) on surface of MMPs; assay procedure required smaller volumes reagents (only 20 μl immune bead); detection could be completed within 30 min; this method 10 pg/ml HCAg-NS3 (sensitivity: 5.2 pg/ml) and...
Objective: To screen and identify differential proteins, analyze lipid metabolism-related proteins pathways, explore their functions biological processes in liver tissue of patients with alcoholic disease using tandem mass tag (TMT) labeling technology. Methods: Liver tissues that met the inclusion criteria were collected. Eight samples from cirrhosis three normal control group screened out. The TMT technique was used to perform signaling pathway enrichment analysis, protein interaction...
Objective: To study the infection status and epidemiological characteristics of parainfluenza virus (PIV) in acute respiratory tract adult cases Shanghai from 2015 to 2021, provide a scientific basis for preventing controlling PIV. Methods: Acute infections were collected 13 hospitals 2021. Relevant information was registered, specimens sampled detect pathogens by multiplex PCR. Results: A total 5 104 included; overall positive rate 29.37% (1 499/5 104). The PIV 2.61% (133/5 104), compared...