A5045737519- HIV Research and Treatment
- HIV/AIDS drug development and treatment
- MicroRNA in disease regulation
- HIV/AIDS Research and Interventions
- RNA modifications and cancer
- RNA Interference and Gene Delivery
- RNA Research and Splicing
- Alzheimer's disease research and treatments
- vaccines and immunoinformatics approaches
- Neuroinflammation and Neurodegeneration Mechanisms
- Hepatitis C virus research
- Immune Cell Function and Interaction
- Virus-based gene therapy research
- Advanced Proteomics Techniques and Applications
- Pneumocystis jirovecii pneumonia detection and treatment
- Advanced biosensing and bioanalysis techniques
University of Zurich
2012-2025
University Hospital of Zurich
2013-2025
Westmead Institute
2009
Westmead Hospital
2009
The University of Sydney
2009
Abstract The HIV-1 reservoir is the major hurdle to a cure. We here evaluate viral and host characteristics associated with size long-term dynamics in 1,057 individuals on suppressive antiretroviral therapy for median of 5.4 years. At population level, decreases diminishing differences over time, but increases 26.6% individuals. Viral blips low-level viremia are significantly slower decay. Initiation ART within first year infection, pretreatment load, ethnicity affect size, less so dynamics....
Background: Despite tremendous advances in antiretroviral therapy (ART) against HIV-1 infections, no cure or vaccination is available. Therefore, discovering novel therapeutic strategies remains an urgent need. In that sense, miRNAs and miRNA therapeutics have moved intensively into the focus of recent HIV-1-related investigations. A strong reciprocal interdependence has been demonstrated between infection changes intrinsic cellular milieu. This interrelationship may direct potential...
Functional microRNAs (miRNAs) are produced from both arms of their precursors (pre-miRNAs). Their abundances vary in context-dependent fashion spatiotemporarily and there is mounting evidence regulatory interplay between them. Here, we introduce chemically synthesized pre-miRNAs (syn-pre-miRNAs) as a general class accessible, easily transfectable mimics pre-miRNAs. These RNA hairpins, identical sequence to natural They differ commercially available miRNA through complete hairpin structure,...
Abstract Background The various classes of small noncoding RNAs (sncRNAs) are important regulators gene expression across divergent types organisms. While a rapidly increasing number sncRNAs has been identified over recent years, the isolation low abundance remains challenging. Virally encoded sncRNAs, particularly those RNA viruses, can be expressed at very levels. This is best illustrated by HIV-1 where virus represent approximately 0.1-1.0% all in infected cells or were found to...
Abstract The host genetic landscape surrounding integrated HIV-1 has an impact on the fate of provirus. Studies analysing integration sites in macrophages are scarce. We studied site patterns monocyte-derived (MDMs) and activated CD4 + T cells derived from seven antiretroviral therapy (ART)-treated HIV-1-infected individuals whose were infected ex vivo with autologous isolated during acute phase infection. A total 1,484 unique analysed. Their distribution human genome features, effects...
Host-directed therapies against HIV-1 are thought to be critical for long term containment of the pandemic but remain elusive. Because infects and manipulates important effectors both innate adaptive immune system, identifying modulations host cell systems in humans during infection may crucial development based therapies. Here, we quantified changes proteome human CD4+ T cells upon infection, vitro vivo. A SWATH-MS approach was used measure primary infected with as well from HIV-1-infected...
Background MiRNAs and other small noncoding RNAs (sncRNAs) are key players in post-transcriptional gene regulation. HIV-1 derived have been described infected cells, but their biological functions still remain to be elucidated. Here, we approached the question whether viral sncRNAs may play a role RNA interference (RNAi) pathway or mRNAs targeted by cellular miRNAs human monocyte macrophages (MDM). Methods The incorporation of and/or target into RNA-induced silencing complex was investigated...
HIV-1 is capable of integrating its genome into that host cell. We examined the influence activation state CD4+ T cells, effect antiretroviral therapy (ART), and clinical stage infection on integration site features selection. sites were sequenced from longitudinally sampled resting activated cells 12 HIV-1-infected individuals. In total, 589 unique analyzed: 147, 391, 51 during primary, chronic, late presentation infection, respectively. As early as primary independent collected off ART,...
HIV-1 penetrates the central nervous system, which is vital for HIV-associated dementia (HAD). But role of cellular infiltration and activation together with HIV in development HAD poorly understood.To study patterns macrophages, CD8+ T cells relation to diverse CNS areas patients without dementia. 46 brain regions from two rapidly progressing severely demented 53 4 HIV+ non-dementia were analyzed. Macrophage cell was assessed using immuno-histochemical analysis anti-HIV (P24), anti-CD8...
The primary hurdle for the eradication of HIV-1 is establishment a latent viral reservoir early after infection. Here, we investigated potential influence human genetic variation on size and its decay rate during suppressive antiretroviral treatment.
Pegylated interferon-alpha (pIFN-α) is suggested to lower human immunodeficiency virus type-1 (HIV-1) DNA load in antiretroviral therapy (ART)-treated patients. We studied kinetics of HIV-1 levels 40 HIV-1/hepatitis C (HCV) coinfected patients, treated with pIFN-α for HCV and categorized into 3 groups according start ART: chronic infection (n = 22), acute 8), no-ART 10). Total 247 peripheral blood mononuclear cell samples were stable before, during, after treatment all groups. Our results...
Abstract Long-lived latently HIV-1-infected cells represent a barrier to cure. We developed dual-fluorescence HIV-1-based vector containing pair of genetic insulators flanking constitutive fluorescent reporter gene study HIV-1 latency. The protective effects these are demonstrated through long-term (up 394 days) stable fluorescence profiles in transduced SUP-T1 cells. Analysis 1,941 integration sites confirmed reproduction patterns. sorted monoclonal representing latent infections and found...
Abstract Background Despite tremendous advances in antiretroviral therapy (ART) against HIV-1 infections no cure or vaccination is available. Therefore, discovering novel therapeutic strategies remains an urgent need. In that sense, miRNAs and miRNA therapeutics have moved intensively into the focus of recent related investigations. A strong reciprocal interdependence has been demonstrated between infection changes intrinsic cellular milieu. This interrelationship may direct potential...
ABSTRACT Introduction A major hurdle to HIV-1 eradication is the establishment of a latent viral reservoir early after primary infection. Several factors are known influence size and decay rate on suppressive antiretroviral treatment (ART), but little about role human genetic variation. Methods We measured at three time points over median 5.4 years, searched for associations between variation two phenotypic readouts: first point its study period. assessed contribution common variants using...