A5071117379- Microbial Natural Products and Biosynthesis
- SARS-CoV-2 and COVID-19 Research
- Viral Infections and Outbreaks Research
- CAR-T cell therapy research
- Immunotherapy and Immune Responses
- COVID-19 Clinical Research Studies
- Bacterial Genetics and Biotechnology
- Genomics and Phylogenetic Studies
- SARS-CoV-2 detection and testing
- Bacteriophages and microbial interactions
- Plant-Microbe Interactions and Immunity
- Biochemical and Molecular Research
- Phytochemistry and Biological Activities
- Essential Oils and Antimicrobial Activity
- Influenza Virus Research Studies
- Cancer Immunotherapy and Biomarkers
- RNA and protein synthesis mechanisms
- Biosensors and Analytical Detection
- Advanced Biosensing Techniques and Applications
- Natural product bioactivities and synthesis
- vaccines and immunoinformatics approaches
- Plant biochemistry and biosynthesis
- Plant Disease Resistance and Genetics
- Animal Virus Infections Studies
- Microbial Metabolic Engineering and Bioproduction
Nanjing University of Chinese Medicine
2023
Fudan University
2013-2021
State Key Laboratory of Genetic Engineering
2015-2018
A novel coronavirus, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), has caused the pandemic of COVID-19. The origin SARS-CoV-2 was associated with zoonotic infections.
The current global pandemic of COVID-19 is caused by a novel coronavirus SARS-CoV-2. SARS-CoV-2 spike protein receptor-binding domain (RBD) the critical determinant viral tropism and infectivity. To investigate whether naturally occurring mutations in RBD have altered receptor binding affinity infectivity, firstly we analyzed silico dynamics between mutated RBDs human ACE2 receptor. Among 1609 genomes strains isolated during early transmission phase, 32 non-synonymous mutants were identified...
The SARS-CoV-2 infection that caused the COVID-19 pandemic quickly spread worldwide within two months. Rapid diagnosis of disease and isolation patients are effective ways to prevent control COVID-19. Therefore, a sensitive immunofluorescent assay method was developed for rapid detection special IgM IgG in human serum 10 min. recombinant nucleocapsid protein 2019 novel coronavirus used as capture antigen. Lanthanide, Eu(III) fluorescent microsphere, qualitatively/semiquantitatively determine...
Streptomyces phage φBT1 integrates its genome into the attB site of host chromosome with attP to generate attL and attR. The integrase belongs large serine recombinase subfamily which directly binds target sites initiate double strand breakage exchange. A recombination directionality factor (RDF) is commonly required for switching integration excision. Here we report characterization RDF protein recombination. RDF, a phage-encoded gp3 gene product (28 KDa), allows efficient active excision...
Streptomyces bacteria are known for producing important natural compounds by secondary metabolism, especially antibiotics with novel biological activities. Functional studies of antibiotic-biosynthesizing gene clusters generally through homologous genomic recombination gene-targeting vectors. Here, we present a rapid and efficient method construction This approach is based on phage φBT1 integrase-mediated multisite in vitro site-specific recombination. Four ‘entry clones’ were assembled into...
A novel CD19xCD3xCD28 trispecific antibody with a tandem single-chain variable fragments (scFv) structure was developed for the treatment of B-cell malignancies.
Natural-product biosynthetic gene clusters are composed of large DNA fragments, which difficult to synthesize in vitro. In this study, a Streptomyces phage site-specific ΦBT1 integrase-mediated recombination reaction was employed obtain the 55 kb erythromycin biosynthesis cluster Saccharopolyspora erythraea. Genome fragments inserted by integrase recognition att-sites (attachment site bacteria and attachment phage) two selectable markers (aphII aac(3)IV gene) on both sides target were...
Cayratia japonica ointment has been used for many years to promote wound healing after perianal abscess surgery. This study aimed determine the skin-permeable and skin-retainable components of topical application intact or broken skin via UPLC-Q-TOF-MS/MS analysis in vitro transdermal assay. Moreover, a combination semi-quantitative molecular docking analyses was performed identify main active probable phases process that they act on. Modified vertical Franz diffusion cells abdominal rats...
Streptomyces sp. fd2-tb can produce streptothricin class antibiotics with broad antimicrobial spectra. To better understand the mechanism of biosynthesis and to assess capacity this strain in secondary metabolism, we report draft genome sequence fd2-tb.
Abstract T-cells, being the major player in adaptive immune system mediating cellular immunity, have become a central focus for engaging fighting against cancer. Their activation is initiated by recognition of antigen/major histocompatibility complexes T-cell receptor (TCR) and enhanced engagement co-stimulatory receptor. In recent years, various immunotherapies deploying immunity made immense progress, including chimeric antigen (CAR)-T therapy, checkpoint blockage, CD3-based bispecific...