A5006992570- Ubiquitin and proteasome pathways
- RNA and protein synthesis mechanisms
- CRISPR and Genetic Engineering
- Aquaculture disease management and microbiota
- Wastewater Treatment and Nitrogen Removal
- RNA regulation and disease
- Nitric Oxide and Endothelin Effects
- Immunotherapy and Immune Responses
- Endoplasmic Reticulum Stress and Disease
- Bioactive Compounds and Antitumor Agents
- Pharmacological Effects of Natural Compounds
- Streptococcal Infections and Treatments
- Atrial Fibrillation Management and Outcomes
- Renin-Angiotensin System Studies
- CAR-T cell therapy research
- RNA modifications and cancer
- Antimicrobial Resistance in Staphylococcus
- Angiogenesis and VEGF in Cancer
- Histone Deacetylase Inhibitors Research
- Algal biology and biofuel production
- Peptidase Inhibition and Analysis
- Photosynthetic Processes and Mechanisms
- Analytical Methods in Pharmaceuticals
- HER2/EGFR in Cancer Research
- Advanced Proteomics Techniques and Applications
Qingdao Agricultural University
2025
East China University of Science and Technology
2020-2024
Shandong University of Technology
2024
First Hospital of China Medical University
2024
China Medical University
2024
North China Electric Power University
2024
Research Institute of Forestry
2023-2024
Chinese Academy of Forestry
2023-2024
First Affiliated Hospital of Henan University of Science and Technology
2024
University of Bonn
2019-2023
Knowledge of elaborate structures protein complexes is fundamental for understanding their functions and regulations. Although cross-linking coupled with mass spectrometry (MS) has been presented as a feasible strategy structural elucidation large multisubunit complexes, this method proven challenging because technical difficulties in unambiguous identification cross-linked peptides determination sites by MS analysis. In work, we developed novel using newly designed MS-cleavable...
Protein–protein interactions (PPIs) are fundamental to the structure and function of protein complexes. Resolving physical contacts between proteins as they occur in cells is critical uncovering molecular details underlying various cellular activities. To advance study PPIs living cells, we have developed a new vivo cross-linking mass spectrometry platform that couples novel membrane-permeable, enrichable, MS-cleavable cross-linker with multistage tandem spectrometry. This strategy permits...
Nedd8 is a small ubiquitin-like protein that can be conjugated to substrate–proteins in process known as neddylation. Although neddylation plays critical regulatory role cell proliferation and development, the spectrum of substrates its interaction network remain poorly understood. To explore pathway at proteome level, we have affinity purified modified associated proteins from HEK293 cells stably expressing GST-Nedd8 employed LC−MS/MS for subsequent identification. A total 496 been...
RNA editing converting cytidines into uridines is a hallmark of gene expression in land plant chloroplasts and mitochondria. Pentatricopeptide repeat (PPR) proteins have key role target recognition, but the functional editosome organelles has remained elusive. Here we show that individual Physcomitrella patens DYW-type PPR alone can perform efficient C-to-U Escherichia coli reproducing moss mitochondrial editing. Single amino acid exchanges DYW domain abolish editing, confirming it as...
The 26S proteasome is the macromolecular machine responsible for ATP/ubiquitin dependent degradation. As aberration in proteasomal degradation has been implicated many human diseases, structural analysis of complex essential to advance our understanding its action and regulation mechanisms. In recent years, cross-linking mass spectrometry (XL-MS) emerged as a powerful tool elucidating topologies large protein assemblies, with unique capability studying complexes cells. To facilitate...
Elimination of aberrantly folded polypeptides from the endoplasmic reticulum (ER) by ER-associated degradation (ERAD) system promotes cell survival under stress conditions. This quality control mechanism requires movement misfolded proteins across ER membrane for targeting to cytosolic proteasome, a process facilitated "holdase" complex, consisting Bag6 and cofactors Ubl4A Trc35. multiprotein complex also participates in several other protein processes. Here, we report SGTA as component...
Structural characterization of proteasome complexes is an essential step toward understanding the ubiquitin-proteasome system. Currently, high resolution structures are not available for 26S holocomplex as well its subcomplex, 19S regulatory particle (RP). Here we have employed a novel integrated strategy combining chemical cross-linking with multistage tandem mass spectrometry to define proximity subunits within yeast RP elucidate topology. This has resulted in identification 174...
We developed sugarcane plants with improved resistance to the borer, Diatraea saccharalis (F). An expression vector pGcry1Ac0229, harboring cry1Ac gene and selectable marker gene, bar, was constructed. This construct introduced into cultivar FN15 by particle bombardment. Transformed plantlets were identified after selection Phosphinothricin (PPT) Basta. Plantlets then screened PCR based on presence of 14 positive identified. Real-time quantitative (RT-qPCR) revealed that copy number in...
Immune and inflammatory responses after stroke are important pathophysiological processes. This study explored the relationship between systemic immune-inflammation index (SII) prognosis. Patients from China National Stroke Registry III were investigated. SII was defined as neutrophils à platelets/lymphocytes, patients divided into four groups according to quartiles based on values. The primary outcome poor functional outcome, assessed by modified Rankin Scale (mRS), an mRS score of â¥3....
Classical swine fever (CSF) is a highly contagious disease caused by classical virus (CSFV). NS5A, non-structural protein of CSFV, plays an important role in regulating viral replication and translation. The purpose this study was to investigate the effects CSFV NS5A on key proteins mitogen-activated kinase (MAPK) phosphoinositide 3-kinase (PI3K)-mechanistic target rapamycin (mTOR) pathways porcine macrophages. In study, lentivirus constructed, 3D4/21 cells were infected. infected for 48 h...
The COP9 signalosome (CSN) is a multi-subunit protein complex that performs critical roles in controlling diverse cellular and developmental processes. Aberrant regulation of the CSN has been shown to lead tumorigenesis. Despite its biological significance, our current knowledge function very limited. To explore biology, we have developed employed new version tag team-based QTAX strategy (quantitative analysis tandem affinity purified vivo cross-linked (X) complexes) by incorporating...
Recent studies have claimed that iron overload was correlated with the risk of hepatocellular carcinoma (HCC), and our previous also demonstrated dandelion polysaccharide (DP) suppressed HCC cell line proliferation via causing cycle arrest inhibiting PI3K/AKT/mTOR pathway, but effect DP on metabolism is still not very clear. Here, we aim to clarify effects underlying mechanism. In this study, found could reduce burden in hepatoma cells grafted tumors. Hepcidin a central regulator metabolism....
Abstract RNA editing processes are strikingly different in animals and plants. Up to thousands of specific cytidines converted into uridines plant chloroplasts mitochondria whereas up millions adenosines inosines animal nucleo-cytosolic RNAs. It is unknown whether these two machineries mutually incompatible. RNA-binding pentatricopeptide repeat (PPR) proteins the key factors organelle cytidine-to-uridine editing. The complete absence PPR mediated cytosolic RNAs might be due a yet barrier...
Myasthenia gravis (MG) is an antibody-mediated autoimmune disease with a prevalence of 150–250 cases per million individuals. Autoantibodies include long-lived antibodies against the acetylcholine receptor (AChR), mainly IgG1 subclass, and IgG4, produced almost exclusively by short-lived plasmablasts, which are prevalent in muscle-specific tyrosine kinase (MuSK) myasthenia gravis. Numerous investigations have demonstrated that MG patients receiving conventional medication today still do not...
ABSTACT Nitric oxide (NO) derived from endothelial NO synthase (eNOS) mediates vascular growth factor (VEGF)‐stimulated cytoskeleton remodeling and migration; however, the underlying mechanisms are elusive. Covalent adduction of a moiety (NO • ) to cysteines called S ‐nitrosylation (SNO) is key signaling pathway. The small actin‐binding protein cofilin‐1 (CFL1) essential for actin remodeling. We investigated whether S‐nitrosylation regulates CFL1 function migration upon VEGF stimulation....
The present study aimed to assess the expression of growth arrest-specific 5 (GAS5) and microRNA (miR)-21 in systemic lupus erythematosus (SLE), attempted explore their association with clinical features. CD4+ T cells were isolated from peripheral blood healthy donors SLE patients by magnetic-activated cell sorting. GAS5 miR-21 levels cluster differentiation (CD)4+ measured reverse-transcription quantitative polymerase chain reaction. results revealed that significantly elevated compared...
Significance Our identification and characterization of the ubiquitin (Ub) Y59-E51 loop have uncovered a pivotal determinant for lysine 48 (K48) linkage-specific Ub chain synthesis catalyzed by Cdc34 E2 Ub-conjugating enzyme. The appears to anchor E2-engaging zone, allowing landing enabling it gain access receptor K48. These findings will provide strong starting point future biochemical structural studies aiming elucidate detailed interactions between E2/E3 enzymes that produce K48 linkage....
Self-healing materials are highly desirable in the nuclear industry to ensure security. Although extensive efforts have been devoted developing self-healing past half century, very limited successes reported for ceramics or metals. Here, we report an intrinsic material of Ti
The mitochondrial C-to-U RNA editing factor PPR56 of the moss Physcomitrium patens is an RNA-binding pentatricopeptide repeat protein equipped with a terminal DYW-type cytidine deaminase domain. Transferred into Escherichia coli, works faithfully on its two native targets, nad3eU230SL and nad4eU272SL, also converts cytidines uridines at over 100 off-targets in bacterial transcriptome. Accordingly, attractive for detailed mechanistic studies heterologous setup, allowing scoring differential...