A5007084343- Sugarcane Cultivation and Processing
- Biofuel production and bioconversion
- Aquaculture disease management and microbiota
- Photosynthetic Processes and Mechanisms
- Fish Biology and Ecology Studies
- Plant Molecular Biology Research
- Plant Gene Expression Analysis
- Aquaculture Nutrition and Growth
- Plant Stress Responses and Tolerance
- Reproductive biology and impacts on aquatic species
- Biosensors and Analytical Detection
- CRISPR and Genetic Engineering
- Natural Products and Biological Research
- Plant biochemistry and biosynthesis
- Heavy metals in environment
- Genomics and Phylogenetic Studies
- Antimicrobial Peptides and Activities
- Plant nutrient uptake and metabolism
- Plant Pathogenic Bacteria Studies
- Plant tissue culture and regeneration
- MicroRNA in disease regulation
- Aluminum toxicity and tolerance in plants and animals
- Animal Nutrition and Physiology
- Turtle Biology and Conservation
- Plant-Microbe Interactions and Immunity
Hunan University of Science and Technology
2018-2025
University of South China
2022-2024
Changsha Central Hospital
2022-2024
Fujian Agriculture and Forestry University
2014-2023
Guangdong Academy of Agricultural Sciences
2023
Soil and Fertilizer Institute of Hunan Province
2022
Sichuan Agricultural University
2005-2019
Jiangxi Academy of Agricultural Sciences
2010
Animal Science Research Institute
2010
Nanyang Medical College
2007
Abstract Despite early domestication around 3000 BC, the evolutionary history of ancient allotetraploid species Brassica juncea (L.) Czern & Coss remains uncertain. Here, we report a chromosome-scale de novo assembly yellow-seeded B. genome by integrating long-read and short-read sequencing, optical mapping Hi-C technologies. Nuclear organelle phylogenies 480 accessions worldwide supported that is most likely single origin in West Asia, 8,000–14,000 years ago, via natural interspecific...
Abstract To meet the demand for detection of foreign genes in genetically modified (GM) sugarcane necessary regulation gene technology, an efficient method with high specificity and rapidity was developed cry1Ac gene, based on loop-mediated isothermal amplification (LAMP). A set four primers designed using sequence along optimized reaction conditions: 5.25 mM Mg 2+ , 4:1 ratio inner primer to outer primer, 2.0 U Bst DNA polymerase a volume 25.0 μL. Three post-LAMP methods (precipitation,...
We developed sugarcane plants with improved resistance to the borer, Diatraea saccharalis (F). An expression vector pGcry1Ac0229, harboring cry1Ac gene and selectable marker gene, bar, was constructed. This construct introduced into cultivar FN15 by particle bombardment. Transformed plantlets were identified after selection Phosphinothricin (PPT) Basta. Plantlets then screened PCR based on presence of 14 positive identified. Real-time quantitative (RT-qPCR) revealed that copy number in...
The SPL (SQUAMOSA promoter-binding protein-like) transcription factor gene family plays a crucial role in the growth of plants, including fruit development. Although has been widely studied many it remains unexplored eggplant, an important Solanaceae vegetable crop. In this study, we performed genome-wide analysis and identified 11 SmSPL genes with 10 motifs eggplant genome, which were assigned to five groups based on amino acid sequences structure SBP domain. addition, expression patterns...
The plant hormone abscisic acid (ABA) has been proved to be a key protector against abiotic stresses at appropriate concentrations. This study aimed investigate the role of ABA in alleviating cadmium (Cd) stress two Brassica napus oil-vegetable dual-purpose (Youfei 1 and Xiangyou 787). Cd disturbed normal growth rapeseed, however, specific concentrations could alleviate effects B. napus. results confirmed that exogenous mitigates negative Cd-induced toxicity by increasing traits, enhancing...
Abstract Seed germination is an important development process in plant growth. The phytohormone abscisic acid (ABA) plays a critical role during seed germination. However, the mechanism of rapeseed response to ABA still elusive. In order understand changes under exogenous treatment, we explored differentially expressed metabolites (DEMs) and genes (DEGs) between mock- ABA-treated seedlings. A widely targeted LC-MS/MS based metabolomics were used identify quantify metabolic germination, total...
Sugarcane (Saccharum spp. hybrids) is considered the most globally important sugar-producing crop and raw material for biofuel. Insect attack a major issue in sugarcane cultivation, resulting yield losses sucrose content reductions. Stem borer (Diatraea saccharalis F.) causes serious worldwide. However, insect-resistant germplasms are not available any collections all over world, molecular mechanism of insect resistance has been elucidated. In this study, cry1Ac transgenic lines were...
Modern sugarcane cultivars are highly polyploid with complex nuclear genomic genetic background, while their mitochondrion (mt) genomes much simpler, smaller and more manageable could provide useful phylogenetic information. In this study, the mt genome of a modern commercial cultivar A1 was sequenced via Illumina Hiseq XTen PacBio Sequel platform. The assembled annotated mitochondrial were composed two circular DNA molecules, one large small, which named Chromosome 1 2. distinct chromosomes...
Allergic rhinitis (AR) is a highly heterogeneous disease, and allergen-specific immunotherapy (AIT) an effective treatment. This study aims to evaluate the circulating mas-related G protein-coupled receptor-X2 (MRGPRX2) matrix metalloproteinase-12 (MMP-12) levels in evaluating disease severity predicting efficacy of SLIT AR patients.We enrolled 110 moderate-severe persist patients (AR group) 40 healthy controls (HC group). Circulating MRGPRX2 MMP-12 were measured, their associations with...
Abstract Smut is a fungal disease with widespread prevalence in sugarcane planting areas. Early detection and proper identification of Sporisorium scitamineum are essential smut management practices. In the present study, four specific primers targeting core effector Pep1 gene S. were designed. Optimal concentrations Mg 2+ , primer Bst DNA polymerase, three important components loop-mediated isothermal amplification (LAMP) reaction system, screened using single factor experiment method L 16...
Genetic engineering offers an attractive alternative in sugarcane breeding for increasing cane and sugar yields as well disease insect resistance. Bar transgenic employing the herbicide tolerance is a useful agronomical trait weed control. In this study, loop-mediated isothermal amplification (LAMP) assay rapid detection of bar gene has been developed evaluated. A set six primers was designed LAMP-based gene. The LAMP reaction conditions were optimized follows: 5.25 mM Mg2+, 6:1 ratio inner...
Anthocyanins confer the wide range of colors for plants and also play beneficial health roles as potentially protective factors against heart disease cancer. Brassica juncea is cultivated an edible oil resource vegetable crop worldwide, thus elucidating anthocyanin biosynthetic pathway would be helpful to improve nutritional quality through breeding cultivating high content varieties. Herein, 129 genes in B. were identified orthologs 41 (ABGs) Arabidopsis thaliana by comparative genomic...
The contamination of soils with cadmium (Cd) has become a serious environmental issue that needs to be addressed. Elucidating the mechanisms underlying Cd accumulation may facilitate development plants accumulate both high and low amounts Cd. In this study, combination phenotypic, physiological, comparative transcriptomic analyses was performed investigate effects different concentrations (0, 5, 10, 30, 50 mg/kg) on Brassica juncea L. Our results suggest B. seedlings had degree tolerance 5...
The VQ motif-containing proteins play a vital role in various processes such as growth, resistance to biotic and abiotic stresses development. However, there is currently no report on the genes sugarcane (Saccharum spp.). Herein, 78 Saccharum spontaneum were identified classified into nine subgroups (I-IX) by comparative genomic analyses. Each subgroup had similar structural conservative motif. These expanded mainly through whole-genome segmental duplication. cis-regulatory elements (CREs)...
Cry1Ac transgenic sugarcane provides a promising way to control stem-borer pests. Biosafety assessment of soil ecosystem for cry1Ac is urgently needed because the important role microorganisms in nutrient transformations and element cycling, however little known. This study aimed explore potential impact on rhizosphere enzyme activities microbial community diversity, also investigate whether gene flow occurs through horizontal transfer. We found no from soil. No significant difference...
Abstract A 10‐week feeding trial aimed to investigate the effects of dietary vitamin C ( l ‐ascorbic acid) on growth, antioxidant activity, and immunity in ricefield eel, Monopterus albus . Six diets were formulated with different levels supplementation (0.1, 17.5, 34.8, 68.6, 139.7, 278.5 mg/kg). The results indicated that weight gain (WG) M. fed was remarkably higher than control diet p < .05), quadratic analysis showed an optimal level 80.66 mg/kg. Compared group, serum lysozyme...
FHY3 and FAR1 serve as positive regulators of the phyA-mediated far-red light signal pathway, influencing plant growth development by regulating expression downstream genes. However, little is known about FAR1/FHY3 family in Brassica species. A total 21 members BnFAR1/FHY3 gene were identified napus genome, exhibiting an uneven distribution across ten B. chromosomes. phylogenetic analysis showed that could be divided into four subfamilies. Putative cis-elements promoter regions also...
Nasopharyngeal carcinoma (NPC) is a malignant epithelial tumor. Among the S100 protein family members, imbalance of calcium-binding A2 (S100A2) was related to pathogenesis several types cancer, and S100A2 has been reported be upregulated in plasma NPC patients; however, its specific role remains unclear. Thus, this study aims determine potential provide novel insights into management. C666-1 NPC/HK-1 cells were transfected with silencing/overexpression (si/oe) constructs. For
Abscisic acid (ABA) interacts antagonistically with brassinosteroids (BRs) to control plant growth and development in response stress. The environmental cues includes hormonal via epigenetic regulation of gene expression. However, the details ABA–BR crosstalk remain largely unknown. Here, we show that JUMONJI-C domain containing histone demethylases (JMJs) coordinate antagonistic interaction between ABA BR signaling pathways during post-germination stage Arabidopsis . blocks ABA-mediated...
NBS-LRR genes constitute one of the largest resistance gene families in plants, which play key roles to pathogens. Although identification and characterization family has been extensively reported various species, a comprehensive analysis eggplant not previously documented. In this study, total 269 SmNBS were identified genome. Based on domain classification phylogenetic analysis, SmNBSs divided into three subgroups 231 CNLs (CC-NBS-LRR), 36 TNLs (TIR-NBS-LRR), 2 RNLs (RPW8-NBS-LRR)....