A5089860371- Sugarcane Cultivation and Processing
- Biofuel production and bioconversion
- Plant Pathogenic Bacteria Studies
- Plant-Microbe Interactions and Immunity
- Plant responses to water stress
- Plant Gene Expression Analysis
- Natural Products and Biological Research
- Plant Stress Responses and Tolerance
- Plant Disease Resistance and Genetics
- Plant Pathogens and Fungal Diseases
- Rice Cultivation and Yield Improvement
- Cocoa and Sweet Potato Agronomy
- Plant nutrient uptake and metabolism
- Molecular Biology Techniques and Applications
- Plant Virus Research Studies
- Photosynthetic Processes and Mechanisms
- Biosensors and Analytical Detection
- Genomics and Phylogenetic Studies
- Plant Molecular Biology Research
- Biochemical and biochemical processes
- Genetics and Plant Breeding
- CRISPR and Genetic Engineering
- Plant tissue culture and regeneration
- Aluminum toxicity and tolerance in plants and animals
- Plant pathogens and resistance mechanisms
Fujian Agriculture and Forestry University
2016-2025
Ministry of Agriculture and Rural Affairs
2020-2025
Chinese Academy of Tropical Agricultural Sciences
2023-2025
Jiaxing University
2025
Yunnan Academy of Agricultural Sciences
2024
Guangxi Academy of Agricultural Science
2024
Fudan University
2024
Yunnan Institute of Tropical Crops
2023
Ministry of Agriculture
2022
Guangxi University
2014-2020
The increasingly used real time quantitative reverse transcription-PCR (qRT-PCR) method for gene expression analysis requires one or several reference gene(s) acting as normalization factor(s). In order to facilitate studies in sugarcane (Saccharum officinarum), a non-model plant with limited genome information, the stability of 13 candidate genes was evaluated. geNorm, NormFinder and deltaCt methods were selecting stably expressed internal controls across different tissues under various...
Sugarcane smut caused by Sporisorium scitamineum is a critical fungal disease in the sugarcane industry. However, molecular mechanistic studies of pathological response to S. are scarce and preliminary. Here, transcriptome analysis induced at 24, 48 120 h was conducted, using an scitamineum-resistant -susceptible genotype (Yacheng05-179 "ROC"22). The reliability Illumina data confirmed real-time quantitative PCR. In total, sequencing eight samples revealed gene annotations 65,852 unigenes....
Sugarcane smut can cause losses in cane yield and sugar content that range from 30% to total crop failure. Losses tend increase with the passage of years. Sporisorium scitamineum is fungus causes sugarcane smut. This has potential infect all species unless a resistant biotrophic fungal pathogens. However, it remains unclear how breaks through cell walls formation black or gray whip-like structures on plants. Here, we report first high-quality genome sequence S. assembled de novo contig N50...
Sugarcane (Saccharum spp. hybrids) is a world-wide cash crop for sugar and biofuel in tropical subtropical regions suffers serious losses cane yield content under salinity drought stresses. Although real-time quantitative PCR has numerous advantage the expression quantification of stress-related genes elaboration corresponding molecular mechanism sugarcane, variation happened across process gene should be normalized monitored by introducing one or several reference genes. To validate...
Catalase is an iron porphyrin enzyme, which serves as efficient scavenger of reactive oxygen species (ROS) to avoid oxidative damage. In sugarcane, the enzymatic activity catalase in a variety (Yacheng05–179) resistant smut pathogen Sporisorium scitamineum was always higher than that susceptible (Liucheng03–182), suggesting may have positive correlation with resistance sugarcane. To understand function at molecular level, cDNA sequence ScCAT1 (GenBank Accession No. KF664183), isolated from...
Abstract To meet the demand for detection of foreign genes in genetically modified (GM) sugarcane necessary regulation gene technology, an efficient method with high specificity and rapidity was developed cry1Ac gene, based on loop-mediated isothermal amplification (LAMP). A set four primers designed using sequence along optimized reaction conditions: 5.25 mM Mg 2+ , 4:1 ratio inner primer to outer primer, 2.0 U Bst DNA polymerase a volume 25.0 μL. Three post-LAMP methods (precipitation,...
Sugarcane smut caused by Sporisorium scitamineum is one of the most severe fungal diseases in sugarcane industry. Using a molecular biological technique to mine resistance genes can provide gene resources for further genetic engineering disease-resistant breeding. Jasmonate ZIM (zinc-finger inflorescence meristem) domain (JAZ) proteins, which involved responses plant pathogens and abiotic stresses, are important signaling molecules jasmonic acid (JA) pathway. Seven differentially expressed...
Abstract Background Alcohol dehydrogenases (ADHs) in plants are encoded by a multigene family. ADHs participate growth, development, and adaptation many plant species, but the evolution function of ADH gene family sugarcane is still unclear. Results In present study, 151 genes from 17 species including 32 Saccharum spontaneum 6 modern cultivar R570 were identified. Phylogenetic analysis demonstrated two groups suggested that these underwent duplication during angiosperm evolution....
Sugarcane smut is a major fungal disease caused by
In plants, catalase (CAT) mainly scavenges H2O2 from reactive oxygen species (ROS) and regulates the growth development. So far, genome-wide identification of CAT gene family in Saccharum has not yet been reported. Here, 16 SsCAT genes were identified based on a spontaneum genome. They clustered into three subfamilies, with closer sharing similar structures. Most proteins contained conserved amino acids, one active catalytic site, heme-ligand signature, peroxisomal targeting signal 1 (PTS1)...
Sugarcane is the most important sugar and energy crop in world. During sugarcane breeding, technology requirement methods are means. As we know, seed cornerstone of development industry. Over past century, with advancement expansion methods, breeding has continued to improve, production realized a leaping growth, providing large amount essential clean for long-term mankind development, especially face future threats world population explosion, reduction available arable land, various biotic...
The sugar industry, which relates to people's livelihood, is strategic and fundamental in the development of agricultural economy. In China, derived from sugarcane accounts for approximately 85% total production. Mechanization "flower" industry. As saying goes "when there are blooming flowers, will be sweet honey." However, due limitations land resources, technology, equipment, organization, management, mechanization throughout production process has not yet brought about economic benefits...
Background Pokkah boeng disease caused by the Fusarium species complex results in significant yield losses sugarcane. Thus, rapid and accurate detection identification of pathogen is urgently required to manage prevent spreading sugarcane pokkah boeng. Methods A total 101 isolates were recovered from samples collected five major production areas China throughout 2012 2013. The causal was identified morphological observation, pathogenicity test, phylogenetic analysis based on fungus-conserved...
Cold is a major abiotic stress limiting the production of tropical and subtropical crops in new areas. Sugarcane (Saccharum spp.) originates from tropics but cultivated primarily sub-tropics where it frequently encounters cold stress. Besides regulating plant growth, miRNAs play an important role environmental adaption. In this study, total 412 sugarcane miRNAs, including 261 known 151 novel were obtained 4 small RNA libraries through Illumina sequencing method. Among them, 62 exhibited...
Abstract Chitinases are pathogensis-related proteins, which play an important role in plant defense mechanisms. The of the sugarcane chitinase family genes remains unclear due to highly heterozygous and aneuploidy chromosome genetic background sugarcane. Ten differentially expressed (belonging class I~VII) were obtained from RNA-seq analysis both incompatible compatible genotypes during Sporisorium scitamineum challenge. Their structural properties expression patterns analyzed. Seven...
Chitinases (EC 3.2.2.14), expressed during the plant-pathogen interaction, are associated with plant defense against pathogens. In present study, a positive correlation between chitinase activity and sugarcane smut resistance was found. ScChi (GenBank accession no. KF664180), Class III gene, encoded 31.37 kDa polypeptide, cloned identified. Subcellular localization revealed targeting to nucleus, cytoplasm plasma membrane. Real-time quantitative PCR (RT-qPCR) results showed that highly in...
Beta-1,3-glucanases (EC 3.2.1.39), commonly known as pathogenesis-related (PR) proteins, play an important role not only in plant defense against fungal pathogens but also physiological and developmental processes. However, a limited number of sugarcane beta-1,3-glucanase genes have been isolated. In the present study, we identified characterized new gene ScGluD2 (GenBank Acc No. KF664181) from sugarcane. An X8 domain was at C terminal region ScGluD2, suggesting beta-1,3-glucan-binding...
In-depth information on sugarcane germplasm is the basis for its conservation and utilization. Data molecular markers are limited Chinese collections. In present study, 20 start codon targeted (SCoT) marker primers were designed to assess genetic diversity among 107 accessions within a local collection. These amplified 176 DNA fragments, of which 163 polymorphic (92.85%). Polymorphic content (PIC) values ranged from 0.783 0.907 with mean 0.861. Unweighted pair group method arithmetic...