A5067674374- Bacteriophages and microbial interactions
- Orthopedic Infections and Treatments
- Antimicrobial Resistance in Staphylococcus
- Microbial infections and disease research
- Bacterial Identification and Susceptibility Testing
- Bacterial biofilms and quorum sensing
- Monoclonal and Polyclonal Antibodies Research
- Infective Endocarditis Diagnosis and Management
- Antibiotic Resistance in Bacteria
- Antibiotics Pharmacokinetics and Efficacy
- Hepatitis B Virus Studies
- Orthopaedic implants and arthroplasty
- Streptococcal Infections and Treatments
- Bacillus and Francisella bacterial research
- Infectious Diseases and Tuberculosis
- Pneumonia and Respiratory Infections
- Biosensors and Analytical Detection
- Antimicrobial Peptides and Activities
- Total Knee Arthroplasty Outcomes
- Vibrio bacteria research studies
- Surgical site infection prevention
- Rabies epidemiology and control
- Viral gastroenteritis research and epidemiology
- Food Allergy and Anaphylaxis Research
- Neonatal and Maternal Infections
Inserm
2017-2025
Université Claude Bernard Lyon 1
2016-2025
École Normale Supérieure de Lyon
2017-2025
Hospices Civils de Lyon
2017-2025
Centre National de la Recherche Scientifique
2017-2025
Centre International de Recherche en Infectiologie
2017-2025
European Society of Clinical Microbiology and Infectious Diseases
2025
Hôpital de la Croix-Rousse
2018-2023
Lyon College
2021
Laboratoire National de Référence
2019
Abstract Bone and joint infections (BJI) are one of the most difficult-to-treat bacterial infection, especially in era antimicrobial resistance. Lytic bacteriophages (phages for short) natural viruses that can selectively target kill bacteria. They considered to have a high therapeutic potential treatment severe BJI, as they also biofilms. Here we report on management patient with pandrug-resistant Pseudomonas aeruginosa spinal abscess who was treated surgery personalized combination phage...
Local injection of a bacteriophages mix during debridement, antibiotics and implant retention ("DAIR") was performed to treat relapsing Staphylococcus aureus chronic prosthetic joint infection (PJI). This salvage treatment safe associated with clinical success. Scientific evaluation the potential benefit as antibiofilm in PJI is now feasible required.
Objectives: To report the management of 3 consecutive patients with relapsing S. aureus prosthetic knee infection (PKI) for whom explantation was not feasible who received a phage therapy during 'Debridement Antibiotics and Implant retention' (DAIR) procedure followed by suppressive antimicrobial therapy. Methods: Each case discussed individually in our reference center French National Agency (ANSM). The lytic activity phages targeting aureus, that produced controlled reproducible process,...
Infection is the most dramatic complication in patients with knee megaprosthesis. Its management more complex comparison primary arthroplasty, a high risk of relapse. Lytic bacteriophages are considered to have potential patient prosthetic joint infection, as it has been demonstrated that they synergistic anti-biofilm activity antibiotics. The Defensive Antibacterial Coating (DAC®) hydrogel available market designed prevent adherence bacteria on and ability transport release anti-bacterial...
Bacteriophages are viruses that specifically target bacteria. They considered to have a high potential in patients with prosthetic joint infection (PJI), as they synergistic anti-biofilm activity antibiotics. We report here the case of an 88-year-old man (63 kg) relapsing Pseudomonas aeruginosa knee infection. The patient had severe alteration general status and was bedridden congestive heart failure. As prosthesis explantation and/or exchange not feasible, we proposed this use phage therapy...
Approximately 15% of patients infected by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) present with forms the disease and require hospitalization in intensive care units, which has been associated high mortality rates. The prevalence bacterial infections these is not well established, more data are needed to guide empiric antibiotic therapy improve patient outcomes.In this prospective multicenter study, we assessed coinfections identified culture from 99 French SARS-Cov-2...
Staphylococcus aureus is responsible for difficult-to-treat bone and joint infections (BJIs). This related to its ability form biofilm be internalized persist inside osteoblasts. Recently, bacteriophage therapy has emerged as a promising option improve treatment of such infections, but data on activity against the specific bacterial lifestyles presented above remain scarce. We evaluated combination three bacteriophages, recently used compassionate in France, S. HG001 model staphylococcal...
Diagnosis of orthopedic device-related infection is challenging, and causative pathogens may be difficult to culture. Metagenomic sequencing can diagnose infections without culture, but attempts detect antimicrobial resistance (AMR) determinants using metagenomic data have been less successful.
Background The potential of phage therapy for the treatment endovascular Staphylococcus aureus infections remains to be evaluated. Methods and Results efficacy a cocktail combining Herelleviridae vB_SauH_2002 Podoviriae 66 was evaluated against methicillin-sensitive S. strain in vitro vivo rodent model experimental endocarditis. Six hours after bacterial challenge, animals were treated with (1) cocktail. (2) subtherapeutic flucloxacillin dosage, (3) combination flucloxacillin, or (4) saline....
Background: Phage therapy a promising antimicrobial strategy to address resistance for infections caused by the major human pathogen Staphylococcus aureus. Development of therapeutic phages use should follow pharmaceutical standards, including selection strictly lytic bacteriophages with high potential and optimization their production process. Results: Here, we describe three novel Silviavirus active against 82% large collection strains (n = 150) representative various...
Abstract Background Staphylococcus epidermidis is a ubiquitous member of the healthy skin and mucous microbiota but also an opportunistic pathogen responsible for various infections, often treated with antibiotics like rifampicin. Resistance to rifampicin in S. arises primarily through nonsynonymous mutations rpoB gene. Objectives To investigate prevalence their association phenotypic resistance clinical isolates from Denmark, France, Sweden. Methods All (N = 942) were whole-genome sequenced...
Methicillin-resistant Staphylococcus aureus (MRSA) is a leading cause of sight-threatening infections in the US. These strains pose significant challenge managing ocular infections, as they frequently exhibit resistance to first-line empirical antibiotics. To assess potential bacteriophages innovative topical therapies for treatment recalcitrant we evaluated vitro antimicrobial activity set anti-S. phages against collection MRSA clinical isolates collected The host range six (V4SA2, V1SA9,...
Background Staphylococcus haemolyticus (SH) is an opportunistic pathogen associated with nosocomial infections, particularly bacteraemia in neonates. Epidemiological trends and genetic diversity of these infections worldwide are largely unknown. Aim To investigate increase SH vascular catheter-related neonates describe the molecular epidemiology France between 2019 2023. Methods We analysed clinical microbiological surveillance data from French national network for central (venous umbilical)...
Exebacase, a recombinantly produced lysin has recently (i) reported proof-of-concept data from phase II study in S. aureus bacteremia and (ii) demonstrated antibiofilm activity vitro against epidermidis . In patients with relapsing multidrug-resistant (MDR) prosthetic knee infection (PKI), the only surgical option is prosthesis exchange. elderly who have undergone several revisions, explantation could be associated definitive loss of function mortality. our BJI reference regional center,...
Optimal treatment of prosthetic joint infection and chronic osteomyelitis consists surgical removal biofilm-embedded bacteria, followed by a 6-12 week course antimicrobial therapy. However, when optimal surgery is not feasible, oral prolonged suppressive antibiotic therapy (PSAT) recommended to prevent prosthesis loosening and/or relapse infection. Since 2010, we have used salvage using off-label subcutaneous (sc) injection β-lactam as PSAT for patients in whom possible.A single-centre...
A 71-year-old man (85 kg) has a past history of vitiligo, ischemic myocardiopathy, and bilateral knee arthroplasties. 1-stage exchange the right prosthetic-joint infection (PJI) was done in 2016 for mechanical prosthetic loosening. massive constrained joint used to compensate bone loss (Supplementary Figure S1A). Iterative postoperative dislocations were followed by occurrence fistula January 2017 loosening S1B) without any pain. Because it impossible imagine 2-stage exchange, debridement...
Abstract Objectives To describe two linezolid-resistant MRSA strains carrying the cfr(B) gene detected in French National Reference Centre for staphylococci. Methods Two isolated from cystic fibrosis patients different hospitals 2017 and 2019 were examined to explore mechanisms of linezolid resistance. Antimicrobial susceptibility was tested using broth microdilution gradient strips. The genetic determinants resistance assessed by a multiplex PCR targeting cfr/cfr(B), optrA poxtA genes,...
Background. Methicillin resistance in Staphylococcus aureus (MRSA) is classically conferred by the acquisition of mecA gene encoding an additional penicillin binding protein with low affinity for beta-lactams. A variant, named mecC, was described 2011. MRSA isolates harboring mecC both animal and human origin have since been collected different European countries. In France, cases were reported 4 dairy farms between 2008 2013 Meurthe-et-Moselle county, all located a 30 km perimeter,...
Among carbapenem-resistant Enterobacterales , metallo-beta-lactamase producing strains represent a growing therapeutic challenge. While the association of aztreonam and ceftazidime-avibactam has been investigated in recent years for treatment infections involving these strains, little to no clinical data support use this bone joint infections. We report two cases complex metallo-beta-lactamase-producing successfully treated at our referral center with 12 weeks continuous infusions through...