A5086474790- Antimicrobial Resistance in Staphylococcus
- Bacteriophages and microbial interactions
- Bacterial Identification and Susceptibility Testing
- Antibiotic Resistance in Bacteria
- Bacterial biofilms and quorum sensing
- Pneumonia and Respiratory Infections
- Hepatitis B Virus Studies
- Clostridium difficile and Clostridium perfringens research
- Monoclonal and Polyclonal Antibodies Research
- Microbial Metabolism and Applications
- Nosocomial Infections in ICU
- Biochemical and Structural Characterization
- Infective Endocarditis Diagnosis and Management
- Mycobacterium research and diagnosis
- Bacterial Infections and Vaccines
- Infection Control in Healthcare
- Bacillus and Francisella bacterial research
- Sepsis Diagnosis and Treatment
- Adrenal Hormones and Disorders
- Orthopedic Infections and Treatments
- Immune Response and Inflammation
- Genomics and Phylogenetic Studies
- Wound Healing and Treatments
- Microbial infections and disease research
Université Claude Bernard Lyon 1
2017-2022
Hospices Civils de Lyon
2017-2022
Centre International de Recherche en Infectiologie
2017-2021
Inserm
2017-2021
Centre National de la Recherche Scientifique
2017-2021
École Normale Supérieure de Lyon
2018-2021
Hôpital de la Croix-Rousse
2018-2021
bioMérieux (France)
2018
Lyon College
2018
Abstract Bone and joint infections (BJI) are one of the most difficult-to-treat bacterial infection, especially in era antimicrobial resistance. Lytic bacteriophages (phages for short) natural viruses that can selectively target kill bacteria. They considered to have a high therapeutic potential treatment severe BJI, as they also biofilms. Here we report on management patient with pandrug-resistant Pseudomonas aeruginosa spinal abscess who was treated surgery personalized combination phage...
Improving timeliness of pathogen identification is crucial to allow early adaptation antibiotic therapy and improve prognosis in patients with pneumonia. We evaluated the relevance a new syndromic rapid multiplex PCR test (rm-PCR) on respiratory samples guide empirical antimicrobial adult community-acquired pneumonia (CAP), hospital-acquired (HAP), ventilator-acquired (VAP).This retrospective multicenter study was conducted four French university hospitals. Respiratory were obtained from...
Abstract Septic shock is accompanied by the development of immune dysfunctions whose intensity and duration are associated with increased risk secondary infections mortality. Although B lymphocytes play a pivotal role in response to infections, no comprehensive exploration circulating cell status has been performed during immunosuppressive phase septic shock. Thus, our aim was extensively characterize phenotype function cells shock, including IL-10 production. Circulating lymphocyte were...
Bacteriophages are viruses that specifically target bacteria. They considered to have a high potential in patients with prosthetic joint infection (PJI), as they synergistic anti-biofilm activity antibiotics. We report here the case of an 88-year-old man (63 kg) relapsing Pseudomonas aeruginosa knee infection. The patient had severe alteration general status and was bedridden congestive heart failure. As prosthesis explantation and/or exchange not feasible, we proposed this use phage therapy...
Staphylococcus aureus is the most prevalent pathogen isolated from diabetic foot infections (DFIs). The purpose of this study was to evaluate its behavior in an vitro model mimicking conditions encountered DFI. Four clinical S. strains were cultivated for 16 weeks a specific environment based on wound-like medium biofilm model. adaptation isolates evaluated as follows: by Caenorhabditis elegans (to virulence); quantitative Reverse Transcription-Polymerase Chain Reaction (qRT-PCR) expression...
Over the last six decades, methicillin-resistant Staphylococcus aureus (MRSA) spread over whole world and has become a global public health threat. Primarily MRSA was restricted to hospitals (hospital-acquired MRSA, HA-MRSA) generally affect patients with predisposing risk factors such as recent hospitalisation, presence of indwelling catheters, admission intensive care unit, exposure patient who is colonised or infected prolonged antibacterial therapy surgery. For three could also be found...
Abstract Community-acquired methicillin-resistant Staphylococcus aureus (CA-MRSA) lineage known as USA300-North American (NA) has become highly prevalent in North America whilst a USA300 variant USA300-LV, harboring mercury resistance element (COMER), dominant South America. We investigated whether pollution, which is common notably because of artisanal gold mining, may explain the local dominance pattern USA300-LV. Density-based estimation epidemic success 250 genomes ST8 revealed that...
Abstract Community-acquired (CA) -as opposed to hospital acquired- methicillin-resistant Staphylococcus aureus (MRSA) lineages arose worldwide during the 1990s. To determine which factors, including selective antibiotic pressure, govern expansion of two major CA-MRSA, namely “USA300” in Northern America and “European ST80” North Africa, Europe Middle East, we explored virulence factor expression, fitness levels with or without antibiotics. The sampled strains were collected a temporal window...
Abstract Background B-lactam resistance in Staphylococci is mediated by mec genes usually diagnosed disc diffusion Cefoxitin test (DDFOX) and PCR testing. Here, we report methicillin-resistant Staphylococcus lugdunensis aureus strains lacking gene misdiagnosed reference methods. Since the are not B-lactamase hyperproducers investigated molecular basis of methicillin resistance. Methods We tested 2 S. isolates (SL1, SL2) collected from distinct blood cultures same patient (SA1, SA2): (i)...
Abstract A pandrug-resistant P. aeruginosa spinal abscess was treated with surgery and personalized phage therapy that added to cefiderocol colistin. After a 2-stage approach, despite bacterial persistence expression of small colony variants, the patient cured local intravenous injections purified bacteriophages as part treatment.