A5102211657- Prenatal Screening and Diagnostics
- Cancer Genomics and Diagnostics
- Epigenetics and DNA Methylation
- Genetic Syndromes and Imprinting
- Genomic variations and chromosomal abnormalities
- Hepatitis B Virus Studies
- Virus-based gene therapy research
- RNA modifications and cancer
- Hepatitis C virus research
- Cancer-related molecular mechanisms research
- Monoclonal and Polyclonal Antibodies Research
- RNA Research and Splicing
- Pregnancy and preeclampsia studies
- Genomics, phytochemicals, and oxidative stress
- Ubiquitin and proteasome pathways
- Viral Infectious Diseases and Gene Expression in Insects
- MicroRNA in disease regulation
- Molecular Biology Techniques and Applications
- Genetic factors in colorectal cancer
- Extracellular vesicles in disease
- Cancer, Stress, Anesthesia, and Immune Response
- Bacillus and Francisella bacterial research
- Birth, Development, and Health
- Rabies epidemiology and control
- Cancer Mechanisms and Therapy
Southern Medical University
2016-2025
Nanfang Hospital
2009-2022
Zhujiang Hospital
2009
Sorafenib is the only approved first line systemic therapy for advanced hepatocellular carcinoma (HCC) in last decade. Tumour resistance to sorafenib has been of major obstacles improve HCC patient survival.We polarised THP-1 cells M1 and M2 macrophages, performed various vitro assays developed sorafenib-resistant xenograft models investigate role tumour-associated macrophages (TAM)-secreted molecules targeted therapy.We demonstrated M2, but not M1, promote proliferation, colony formation...
In rheumatoid arthritis(RA) pathogenesis, activated RA fibroblast-1ike synoviocytes (RA-FLSs) exhibit similar proliferative features as tumor cells and subsequent erosion to cartilage will eventually lead joint destruction. Therefore, it is imperative search for compounds, which can effectively inhibit the abnormal activation of RA-FLSs, retard progression.3′3-Diindolylmethane (DIM), major product acid-catalyzed oligomerization indole-3-carbinol from cruciferous vegetables, has been reported...
// Bin Xiao 1, * , Daxiang Chen 1 Shuhong Luo 2, 3 Wenbo Hao Fangyan Jing 4 Tiancai Liu Suihai Wang Yan Geng 5 Linhai Li 6 Weiwen Xu Yajie Zhang 7 Xiaoqing Liao Daming Zuo 8 Yingsong Wu Ming Qiang Ma State Key Laboratory of Organ Failure Research, Institute Antibody Engineering, School Biotechnology, Southern Medical University, Guangzhou, 510515, Guangdong, China 2 RayBiotech, Inc., Guangzhou 510600, Norcross, GA 30092, USA Department Anorectal Surgery, Nanfang Hospital, Guangdong Province,...
Abstract Placenta‐origin pregnancy complications, including preeclampsia (PE), gestational diabetes mellitus (GDM), fetal growth restriction (FGR), and macrosomia (MA) are common occurrences in pregnancy, resulting significant morbidity mortality for both mother fetus. However, despite their frequency, there no reliable methods the early diagnosis of these complications. Since cfDNA is mainly derived from placental trophoblasts maternal hematopoietic cells, it might have information gene...
Rheumatoid arthritis (RA) is a chronic and progressive autoimmune disease, in which activated RA fibroblast-1ike synoviocytes (RA-FLSs) are one of the main responsible for inducing morbidity. Previous reports have shown that RA-FLSs proliferative features similar to cancer cells, addition causing cartilage erosion eventually causes joint damage. Thus, new therapeutic strategies drugs, can effectively contain abnormal hyperplasia restrain development, necessary treatment RA. Tanshinone IIA...
Profiling of steroid hormones is incredibly valuable in clinical settings for diagnosing endocrine disorders. However, the presence matrix effects and labor-intensive manual work LC-MS/MS analysis has hindered its routine application. In present study, a highly efficient automated magnetic bead extraction method was developed to address quantitatively profile 15 human serum. Octadecyl (C18) N-vinylpyrrolidone divinylbenzene (HLB) modified beads were compared enriching steroids from Following...
Translationally controlled tumor protein (TCTP) plays a major role in broad array of biological processes. However, the TCTP-related process and interactive proteins still remain poorly characterized. In present study, we found that knockdown TCTP inhibited proliferation, migration, invasion activities LoVo cells vitro vivo. The whole-cell proteomes were compared by 2D gel electrophoresis before after TCTP. Alterations 27 detected their identities revealed mass spectrometry analysis....
Abstract Extracellular vesicles (EVs) mediate intercellular communication via transferring proteins and other biological molecules have been recently investigated as biomarkers of disease. Sensitive specific are required for lung cancer diagnosis prognosis. The present study screens abnormal EV in non‐small cell (NSCLC) using a quantitative proteomics strategy involving LC‐MS/MS to identify ideal NSCLC diagnosis. EVs enriched from the sera early advanced patients healthy controls culture...
Hepatocellular carcinoma (HCC) is one of the most lethal cancers worldwide due to a high rate tumour metastasis and disease recurrence. In physiological conditions, tetraspanins interact with specific partner proteins in tetraspanin-enriched microdomains regulate their subcellular localization function. However, function Tspan5 pathological processes, particularly cancer biology its clinical significance, are still unclear. Here, we describe that expression significantly associated some...
Patients suffering from advanced stage hepatocellular carcinoma (HCC) often exhibit a poor prognosis or dismal clinical outcomes due to ineffective chemotherapy multi-drug resistance (MDR) process. Thus, it is urgent develop new chemotherapeutic sensitivity testing system for HCC treatment. The presence study investigated the potential application of novel sensitivity-testing based on collagen gel droplet embedded 3D-culture (CD-DST).Primary cells were separating surgical resection specimens...
Objective The fraction of circulating cell-free fetal (cff) DNA in maternal plasma is a critical parameter for aneuploidy screening with non-invasive prenatal testing, especially those samples located equivocal zones. We developed an approach to quantify cff fractions directly sequencing data, and increased DNAs by optimizing library construction procedure. Methods Artificial mixture (360), known fractions, were used develop method determine through calculating the proportion Y chromosomal...
Breast cancer is the second cause of cancer-associated death among women and seriously endangers women's health. Therefore, early identification breast would be beneficial to At present, circular RNA (circRNA) not only exists in extracellular vesicles (EVs) plasma, but also presents distinct patterns under different physiological pathological conditions. we assume that circRNA could used for diagnosis cancer. Here, developed classifiers relied on 259 samples, including 144 patients 115...
YTH domain family (YTHDF) 2 acts as a "reader" protein for RNA methylation, which is important in tumor regulation. However, the effect of YTHDF2 liver hepatocellular carcinoma (LIHC) has yet to be elucidated.We explored role LIHC based on publicly available datasets [The Cancer Genome Atlas (TCGA), International Consortium (ICGC), and Gene Expression Omnibus (GEO)]. A bioinformatics approach was employed analyze YTHDF2. Logistic regression analyses were applied correlation between...
Abstract Cisplatin (DDP) resistance is a major challenge in treating ovarian cancer patients. A recently discovered enzyme called dCTP pyrophosphatase 1 (DCTPP1) has been implicated regulating characteristics, including drug responses. In this study, we aimed to understand the role of DCTPP1 progression and cisplatin response. Using publicly available databases, analysed expression clinical significance cancer. Our bioinformatics analysis confirmed that significantly overexpressed closely...
Background Cytokeratin 19 fragment antigen (CYFRA 21–1) is used to diagnose and monitor neoplasms. However, the main disadvantages of currently available CYFRA 21–1 assays include heterogenous technology, being time‐consuming, having low through‐put with insensitivity. This study investigated use amplified luminescent proximity homogeneous immunoassay (AlphaLISA) for quantization in human serum. Methods The AlphaLISA kit was developed based on AlphaScreen detection technology two different...
ABSTRACT Time‐resolved fluorometry of lanthanide chelates is one the most useful non‐isotopic detection techniques and has been used in numerous applications biomedical science. We developed a time‐resolved fluoroimmunoassay (TRFIA) to quantify α‐fetoprotein (AFP) hepatitis B virus surface antigen (HBsAg) human serum. Based on two‐site sandwich protocol, monoclonal antibodies (McAbs) against AFP HBsAg were co‐coated 96 microtitration wells tracer McAbs labeled with europium (Eu) samarium...
Objective: To understand the role of WFDC2 in metastasis ovarian cancer. Methods: By knockdown or overexpression , we demonstrated epithelial–mesenchymal transition (EMT). Results: We that stable suppressed EMT along with upregulation E-cadherin and downregulation Vimentin. In addition, decreases matrix metalloproteinase-2 (MMP-2) expression vitro cell model vivo nude mice xenografts. The correlation MMP-2 clinical sample confirmed was tightly correlated development tumor. More importantly,...
Super-enhancers (SEs) are enriched with a cluster of mediator binding sites, which major contributors to cell-type-specific gene expression. Currently, large quantity long non-coding RNAs has been found be transcribed from or interact SEs, constitute super-enhancer associated (SE-lncRNAs). These SE-lncRNAs play essential roles in transcriptional regulation through controlling SEs activity regulate broad range physiological and pathological processes, especially tumorigenesis. However, the...
Cisplatin resistance hinders the improvement of prognosis patients with ovarian cancer. induces cancer cell apoptosis by inducing reactive oxygen species (ROS). dCTP pyrophosphatase 1 (DCTPP1) is a newly discovered dNTP pyrophosphatase. This study aimed to identify role DCTPP1 in oxidative stress and cisplatin response Our results indicates cisplatin-induced ROS generation was responsible for upregulation cells, whereas knockdown significantly enhanced sensitivity cells cisplatin, reflect...