A5027197117- Advanced biosensing and bioanalysis techniques
- Amyotrophic Lateral Sclerosis Research
- DNA and Nucleic Acid Chemistry
- Alzheimer's disease research and treatments
- RNA Interference and Gene Delivery
- Parkinson's Disease Mechanisms and Treatments
- Molecular Sensors and Ion Detection
- Physics of Superconductivity and Magnetism
- biodegradable polymer synthesis and properties
- Pharmacogenetics and Drug Metabolism
- Inflammatory Bowel Disease
- Computational Drug Discovery Methods
- Magnetic and transport properties of perovskites and related materials
- RNA and protein synthesis mechanisms
- Cholinesterase and Neurodegenerative Diseases
- 3D Printing in Biomedical Research
- Electrowetting and Microfluidic Technologies
- Advanced Drug Delivery Systems
- Biochemical Acid Research Studies
- Drug Transport and Resistance Mechanisms
- Neurogenetic and Muscular Disorders Research
- DNA and Biological Computing
- Neutropenia and Cancer Infections
- Nanoparticle-Based Drug Delivery
- Peptidase Inhibition and Analysis
Massachusetts Institute of Technology
2000-2024
Harvard University
2020-2022
Brigham and Women's Hospital
2020-2022
Draper Laboratory
2021
Beijing National Laboratory for Molecular Sciences
2014-2018
Baylor University
2012-2018
Chinese Academy of Sciences
2014-2018
University of Chinese Academy of Sciences
2014-2018
Institute of Chemistry
2014-2018
State Key Laboratory for Structural Chemistry of Unstable and Stable Species
2014-2016
Lipid-like nanoparticles (LNPs) have potential as non-viral delivery systems for mRNA therapies. However, repeated administrations of LNPs may lead to accumulation materials and associated toxicity. To address this challenge, we developed biodegradable lipids which improve clearance reduce We modify the backbone structure Dlin-MC3-DMA by introducing alkyne ester groups into lipid tails. evaluate performance these when co-formulated with other amine containing lipid-like materials....
Abstract RNA G-quadruplexes (G4s) play important roles in translational regulation, mRNA processing events and gene expression. Therefore, a fluorescent probe that is capable of efficiently recognizing G-quadruplex structures among other forms highly desirable. In this study, water-soluble fluorogenic dye (i.e., Thioflavin T (ThT)) was employed to recognize using UV–Vis absorption spectra, fluorescence spectra emission lifetime experiments. By stacking on the G-tetrad, ThT exhibited specific...
The amino acid substitution or post-translational modification of a cytosolic protein can cause unpredictable changes to its electrophoretic mobility during SDS-PAGE. This type "gel shifting" has perplexed biochemists and biologists for decades. We identify mechanism that predominates among set ALS (amyotrophic lateral sclerosis) mutant hSOD1 (superoxide dismutase) proteins, post-translationally modified homologous SOD1 proteins from different organisms. By first comparing how 39...
RNA G-quadruplexes (G4s) are one of the key components transcriptome that act as efficient post-transcriptional regulatory elements in living cells. To conduct further studies unique biological functions G4s, techniques need to be developed can efficiently recognize G4 structures under various conditions, fixed cells and cells, well vitro. This paper presents development such a method, new technique using cyanine dye called CyT, which detect both canonical non-canonical from test tubes human...
A targeted coating of polydopamine polymerized in situ the small intestine enables drug delivery and modulation nutrient absorption pigs.
The reactivity of asparagine residues in Cu, Zn superoxide dismutase (SOD1) to deamidate aspartate remains uncharacterized; its occurrence SOD1 has not been investigated, and the biophysical effects deamidation on are unknown. Deamidation is, nonetheless, chemically equivalent Asn-to-Asp missense mutations that cause amyotrophic lateral sclerosis (ALS). This study utilized computational methods identify three wild-type (WT) (i.e., N26, N131, N139) predicted undergo significant >20%) time...
Inactive ingredients and generally recognized as safe compounds are regarded by the US Food Drug Administration (FDA) benign for human consumption within specified dose ranges, but a growing body of research has revealed that many inactive might have unknown biological effects at these concentrations alter treatment outcomes. To speed up such discoveries, we apply state-of-the-art machine learning to delineate currently ingredients-focusing on P-glycoprotein (P-gp) uridine...
Logic gates based on an i-motif structure, which was induced by H<sup>+</sup>/Ag<sup>+</sup> and recognized a cyanine dye, have been designed.
Recent reports suggest that the nucleation and propagation of oligomeric superoxide dismutase-1 (SOD1) is effectively stochastic in vivo vitro. This perplexing kinetic variability—observed for other proteins frequently attributed to experimental error—plagues attempts discern how SOD1 mutations post-translational modifications linked amyotrophic lateral sclerosis (ALS) affect aggregation. study used microplate fluorescence spectroscopy dynamic light scattering measure rates fibrillar...
Abstract Targeting areas of inflammation offers potential therapeutic and diagnostic benefits by maximizing drug imaging marker on‐target effects while minimizing systemic exposure that can be associated with adverse side effects. This strategy is particularly beneficial in the management inflammatory bowel disease (IBD). Here an inflammation‐targeting (IT) approach based on heparin‐coated human serum albumin nanoparticles (HEP‐HSA NPs) utilize increased intestinal permeability changes...
The chemical and physical mechanisms by which gyrating beads accelerate amyloid fibrillization in microtiter plate assays are unclear. Identifying these will help optimize high-throughput screening for molecules mutations that modulate aggregation might explain why different research groups report rates of identical proteins. This article investigates how the rate superoxide dismutase-1 (SOD1) is affected 12 with a wide range hydrophobicity, mass, stiffness, topology but diameter. All were...
The acylation of lysine residues in superoxide dismutase-1 (SOD1) has been previously shown to decrease its rate nucleation and elongation into amyloid-like fibrils linked amyotrophic lateral sclerosis. chemical mechanism underlying this effect is unclear, i.e. hydrophobic/steric effects versus electrostatic effects. Moreover, the degree which might alter prion-like seeding SOD1 vivo not addressed. Here, we acylated a fraction with groups variable hydrophobicity, charge, conformational...