A5056920489- Ocular Oncology and Treatments
- Hippo pathway signaling and YAP/TAZ
- RNA modifications and cancer
- RNA Research and Splicing
- Ubiquitin and proteasome pathways
- RNA and protein synthesis mechanisms
- Cancer-related Molecular Pathways
- Retinal Development and Disorders
- Angiogenesis and VEGF in Cancer
- Corneal Surgery and Treatments
- Cellular transport and secretion
- Advanced Proteomics Techniques and Applications
Université Paris Sciences et Lettres
2019-2023
Institut Curie
2019-2023
Abstract Genes involved in 3′-splice site recognition during mRNA splicing constitute an emerging class of oncogenes. SF3B1 is the most frequently mutated factor cancer, and mutants corrupt branchpoint leading to usage cryptic sites subsequent aberrant junctions. For a comprehensive determination alterations this pattern, we performed pan-TCGA screening for SF3B1-specific acceptor usage. While patterns were explained by mutations, also detected nine wild-type tumors (including five lung...
Uveal Melanoma (UM) is a rare and malignant intraocular tumor with dismal prognosis. Even if radiation or surgery permit an efficient control of the primary tumor, up to 50% patients subsequently develop metastases, mainly in liver. The treatment UM metastases challenging patient survival very poor. most recurrent event activation Gαq signaling induced by mutations GNAQ/11. These activate downstream effectors including protein kinase C (PKC) mitogen-activated kinases (MAPK). Clinical trials...
Uveal melanoma (UM) is a rare and malignant intraocular tumour with dismal prognosis. Despite good control of the primary by radiation or surgery, up to 50% patients subsequently develop metastasis for which no efficient treatment yet available. To identify therapeutic opportunities, we performed an in vitro screen 30 combinations different inhibitors pathways that are dysregulated UM. Effects drug on viability, cell cycle apoptosis were assessed eight UM lines. The best synergistic further...
The hotspot mutations of SF3B1, the most frequently mutated splicing gene in cancers, contribute to oncogenesis by corrupting mRNA splicing. Further SF3B1 have been reported cancers but their consequences remain unclear. Here, we screened for vicinity region tumors. We then performed in-silico prediction functional outcome followed in-cellulo modelling different mutants. show that cancer-associated present varying are loosely predicted algorithms. Analysis tertiary structure mutants revealed...
SF3B1 mutations are recurrent in cancer and result aberrant splicing of a previously defined set genes. Here, we investigated the fate transcripts induced by mutant related functional consequences. We first demonstrate that does not alter global nascent protein synthesis, suggesting target-dependent Polysome profiling revealed 35% aberrantly spliced more translated than their corresponding canonically transcripts. This mostly occurs genes with enriched metabolic functions. Furthermore,...