A5103247991- Cancer Cells and Metastasis
- Cancer-related Molecular Pathways
- MicroRNA in disease regulation
- Cancer Genomics and Diagnostics
- RNA modifications and cancer
- Protein Degradation and Inhibitors
- Cellular Mechanics and Interactions
- Lung Cancer Treatments and Mutations
- Cancer-related gene regulation
- Cancer, Hypoxia, and Metabolism
- Circular RNAs in diseases
- Protein Kinase Regulation and GTPase Signaling
- RNA Interference and Gene Delivery
- 3D Printing in Biomedical Research
- Bioactive natural compounds
- Signaling Pathways in Disease
- Immunotherapy and Immune Responses
- Mitochondrial Function and Pathology
- Peptidase Inhibition and Analysis
- Heme Oxygenase-1 and Carbon Monoxide
- Molecular Biology Techniques and Applications
- Head and Neck Cancer Studies
- HER2/EGFR in Cancer Research
- Cancer-related molecular mechanisms research
- Receptor Mechanisms and Signaling
University of Virginia
2023
The Ohio State University Wexner Medical Center
2012-2022
The Ohio State University
2012-2018
The Ohio State University Comprehensive Cancer Center – Arthur G. James Cancer Hospital and Richard J. Solove Research Institute
2012-2016
University of Michigan
2013
Head and neck squamous cell carcinoma (HNSCC) is the sixth most prevalent cancer worldwide with about 600,000 new cases diagnosed in last year. Our laboratory showed that miR-107 expression reduced functions as a tumor suppressor gene HNSCC suggesting potential application of novel anticancer therapeutic. In this study, we determined efficiency efficacy cationic lipid nanoparticles to deliver pre-miR-107 (NP/pre-miR-107) cells vitro vivo. NP/pre-miR-107 increased delivery into by greater...
BACKGROUND Human papillomavirus 16 (HPV16) is a major risk factor for the development of head and neck squamous cell carcinoma (HNSCC), particularly oropharyngeal (OPSCC). Cancer stem cells (CSCs) are resistant to conventional therapies, it postulated that they responsible disease recurrence and/or progression. Because prognoses patients with HPV16‐positive HPV‐negative HNSCC distinct, authors sought determine whether differences in number CSCs could account this clinical observation....
Head and neck squamous cell carcinoma (HNSCC) is the sixth most prevalent cancer worldwide with about 600,000 new cases diagnosed in last year. The incidence of human papillomavirus-positive head (HPV-positive HNSCC) has rapidly increased over past 30 years prompting suggestion that an epidemic may be on horizon. Therefore, there a clinical need to develop alternate therapeutic strategies manage growing number HPV-positive HNSCC patients. TriCurin composition three food-derived polyphenols...
Vascular smooth muscle cell (VSMC) proliferation promotes intimal hyperplasia (IH) in occluding vascular diseases. Here we identified a positive role of ALDH1A3 (an aldehyde dehydrogenase) this pro-IH process. The expression ALDH1A3, but not that 18 other isoforms the ALDH family, was substantially increased cytokine-stimulated VSMCs. PDGF(BB) stimulated VSMC total activity and proliferation, whereas silencing abolished effect. also diminished two matricellular proteins (TNC1 ESM1),...
Human papillomavirus-associated head and neck squamous cell carcinoma (HPV+ HNSCC) is recognized as a distinct disease with unique etiology clinical features. Current standard of care therapeutic modalities are identical for HPV+ HPV- HNSCC thus, there remains an opportunity to develop innovative pharmacologic approaches exploit the inherent vulnerabilities HNSCC. In this study, using inducible HPVE6E7 knockdown system, we found that cells addicted HPVE6E7, such loss these viral oncogenes...
Abstract Epithelial-restricted with serine box (ESX), a member of the ETS transcription factor family, is elevated and regulates EGFR in head neck squamous cell carcinoma (HNSCC). However, molecular mechanisms that contribute to ESX dysregulation remain be elucidated. In this study, silico analysis 3′-untranslated region (UTR) predicted two miR-124–binding sites. Delivery miR-124 inhibited 3′UTR ESX-driven reporter activity by 50% (P < 0.05) confirming as direct target miR-124. Loss...
Abstract p53 and aldehyde dehydrogenase (ALDH) have been implicated in key tumorigenesis processes including cancer initiating cell (CIC) maintenance; however, the relationship between these two mediators remains poorly defined. In this study, ALDH isoform expression diversity was revealed CICs with disparate functional states: gain of function, high risk mutation (p53HRmut) wildtype (p53WT) inactivated by human papillomavirus 16 (HPV16) E6 oncogene. Interrogation head neck squamous...
Protein kinase C ε (PKC ) signals through RhoA to modulate cell invasion and motility. In this study, the multifaceted interaction between PKC was defined. Phosphopeptide mapping revealed that phosphorylates at T127 S188. Recombinant bound recombinant in absence of ATP indicating association does not require an active ATP-docked conformation. Activation resulted a dramatic coordinated translocation from cytoplasm membrane using time-lapse fluorescence microscopy. Stoichiometric FRET analysis...
EGF receptor (EGFR) is elevated in more than 90% of head and neck squamous cell carcinoma (HNSCC). However, a majority patients with HNSCC do not respond to anti-EGFR therapeutics. Insensitivity EGFR inhibitors may be due kinase-independent actions and/or activation Her2. Strategies reduce Her2 protein levels concert an optimal approach enhance the efficacy current molecules. In this study, knockdown epithelial-restricted serine box (ESX) decreased promoter activity, expression, levels. ESX...
Abstract Traditionally a pharmacologic target for antipsychotic treatment, the sigma-2 receptor (S2R) was recently implicated in cholesterol homeostasis. Here we investigated transcriptional regulation of S2R by Bromo/ExtraTerminal epigenetic reader family (BETs, including BRD2, 3, 4) upon perturbation. Cytosolic deprivation induced using an export blocker lysosomal ARPE19 cells. This condition upregulated mRNA and protein levels S2R, SREBP2 but not SREBP1, transcription factors key to...
Open vascular reconstructions such as bypass are common treatments for cardiovascular disease. Unfortunately, neointimal hyperplasia (IH) follows, leading to treatment failure which there is no approved therapy. Here we combined the strengths of tailoring nanoplatforms open and targeting new epigenetic mechanisms. We produced adhesive nanoparticles (ahNP) that could be pen-brushed immobilized on adventitia sustainably release pinometostat, an inhibitor drug selective writer DOT1L catalyzes...
Abstract Objectives: Nanog is a transcription factor intimately involved in embryonic stem cell pluripotency. Emerging evidence indicates that plays critical role oncogenesis, part through regulation of the cancer initiating population. We investigated post-translational phosphorylation as regulatory mechanism to control function head and neck squamous carcinoma (HNSCC). Methods: Liquid chromatography-mass spectrometry/mass spectrometry was used identify PKC∈-mediated sites on Nanog....