A5102709520- Receptor Mechanisms and Signaling
- Pharmacological Receptor Mechanisms and Effects
- Sulfur-Based Synthesis Techniques
- Protein Degradation and Inhibitors
- Catalytic Cross-Coupling Reactions
- Catalytic C–H Functionalization Methods
- Neuropeptides and Animal Physiology
- Fullerene Chemistry and Applications
- Ubiquitin and proteasome pathways
- Chemical Synthesis and Reactions
- Neuroscience and Neuropharmacology Research
- Extraction and Separation Processes
- Signaling Pathways in Disease
- Tryptophan and brain disorders
- Neuroinflammation and Neurodegeneration Mechanisms
- Graphene research and applications
- Catalytic Alkyne Reactions
- RNA regulation and disease
- MicroRNA in disease regulation
- Autophagy in Disease and Therapy
- NF-κB Signaling Pathways
- Food Quality and Safety Studies
- 14-3-3 protein interactions
- Synthesis and Catalytic Reactions
- Cancer-related molecular mechanisms research
China Pharmaceutical University
2020-2025
University of Virginia
2020-2025
Harbin Medical University
2014-2024
China Tobacco
2020-2024
First Affiliated Hospital of Harbin Medical University
2014-2024
State Key Laboratory of Natural Medicine
2023
Northeastern University
2006-2023
Guangdong Medical College
2022
Kunming Institute of Zoology
2022
Chinese Academy of Sciences
2016-2022
Autophagosome-lysosome fusion is a common critical step in various forms of macroautophagy/autophagy including mitophagy, the selective degradation mitochondria. Regulations this process remain poorly defined. Here we have determined role SIGMAR1, unique endoplasmic reticulum membrane protein. Knockout Sigmar1 impaired mitochondrial clearance without altering PINK1-PRKN/Parkin signaling, mouse retinal explants and cultured cells treated with carbonyl cyanide m-chlorophenyl hydrazone (CCCP)...
Osimertinib resistance is an unmet clinical need for the treatment of non-small cell lung cancer (NSCLC), and main mechanism tertiary C797S mutation epidermal growth factor receptor (EGFR). To date, there no inhibitor approved Osimertinib-resistant NSCLC. Herein, we reported a series derivatives as fourth-generation inhibitors which were rationally designed. Top candidate D51 potently inhibited EGFRL858R/T790M/C797S mutant with IC50 value 14 nM suppressed proliferation H1975-TM cells nM,...
<title>Abstract</title> Cyclic oligonucleotide-based antiphage signaling systems (CBASS) are widespread bacterial immune that trigger host suicide via cyclic nucleotide-activated effectors. The predominant strategy to induce cell death in CBASS is membrane disruption. Here, we demonstrate patatin-like phospholipase CapV, the most abundant effector, relocates and cleaves phospholipids at pole upon 3’3’-cGAMP binding, inducing polarized disruption death. Using cryo-EM, reveal apo-CapV adopts...
Abstract Background This study aimed to evaluate the association of circular RNA La‐related RNA‐binding protein 4 (circ‐LARP4) with clinical features and prognosis in osteosarcoma patients, further explore its effect on chemosensitivity cells. Methods Seventy‐two patients Enneking stage IIA‐IIB who underwent resection were consecutively enrolled, then, tumor tissues non‐tumor obtained. Circ‐LARP4 tissue/non‐tumor tissue was detected by quantitative polymerase chain reaction. After circ‐LARP4...
Prostate cancer (PCa) is a common male with high incidence and mortality, hormonal therapy as the major treatment for PCa patients troubled by inevitable resistance that makes us identify novel targets PCa. Dual-specificity tyrosine phosphorylation-regulated kinase 2 (DYRK2) was found to be an effective target of PCa, but research on its inhibitors rather little. In this work, potent DYRK2 inhibitor 43 (IC50 = 0.6 nM) acquired through virtual screening structural optimization, which...
T helper (Th) cells are closely involved in vascular inflammation, endothelial dysfunction, and atherogenesis, which the hallmarks of aortic dissection (AD). This study aimed to evaluate clinical value Th1, Th2, Th17 cell measurements Stanford type A AD patients.Stanford patients (N=80) non-AD with chest pain (N = 40) were recruited. Then, peripheral blood CD4+ from all participants detected by flow cytometry. The 30-day mortality was recorded.Th1 higher, while Th2 lower compared (all p <...
The biogenesis of photosystem I (PSI), cytochrome b 6 f (Cytb f) and NADH dehydrogenase (NDH) complexes relies on the spatially temporally coordinated expression translation both nuclear chloroplast genes. Here we report identification regulator 1 (PBR1), a nuclear-encoded RNA-binding protein that regulates concerted NDH, PSI Cytb complexes. We identified Ycf1, one two largest genome-encoded open reading frames as direct downstream target PBR1. Biochemical molecular analyses reveal PBR1 Ycf1...
Aberrant smooth muscle cell (SMC) plasticity is etiological to vascular diseases. Cholesterol induces SMC phenotypic transition featuring high LGALS3 (galectin-3) expression. This proatherogenic process poorly understood for its molecular underpinnings, in particular, the mechanistic role of sterol regulatory-element binding protein-1 (SREBP1), a master regulator lipid metabolism. Herein we show that cholesterol loading stimulated SREBP1 expression mouse, rat, and human SMCs. positively...
The 90-kDa heat shock protein HSP90AA1 is critical for the stability of several proteins that are important tumor progression and thus, a promising target cancer therapy. Selenosemicarbazone metal complexes have been shown to possess anticancer activity through an unknown molecular mechanism. MTT assay, fluorescence-activated cell sorting, fluorescent microscopy were used analyze mechanism anti-cancer selenosemicarbazone complexes. Additionally, RNA-seq was applied identify transcriptional...
The sigma-2 receptor (S2R) has long been pharmacologically targeted for antipsychotic treatment and tumor imaging. Only recently was it known its coding gene role implicated in cholesterol homeostasis. Here, we have investigated the transcriptional control of S2R by Bromo/ExtraTerminal epigenetic reader family (BETs, including BRD2, 3, 4) upon perturbation. Cholesterol deprivation induced ARPE19 cells using a blocker lysosomal export. This condition up-regulated mRNA protein, also SREBP2 but...
Obesity is increasing at epidemic rates across the US and worldwide, as are its co-morbidities, including type-2 diabetes cardiovascular disease. Thus, targeted interventions to reduce prevalence of obesity utmost importance. The sigma-1 receptor (S1R) sigma-2 (S2R; encoded by Tmem97) belong same class drug-binding sites, yet they genetically distinct. There multiple ongoing clinical trials focused on sigma receptors, targeting diseases ranging from Alzheimer’s disease through chronic pain...
Transition-metal-catalyzed selective hydroarylation of alkynes represents a state-of-the-art approach in organic chemistry. Herein, we report the reaction symmetrical 1,3-diynes and arylboronic acids, with Pd(OAc)
Nickel-catalyzed amidation of aryl alkynyl acids using tetraalkylthiuram disulfides as the amine source is described, affording a series amides in good to excellent yields under mild conditions. This general methodology provides an alternative pathway for synthesis useful operationally simple manner, which shows its practical synthetic value organic synthesis. The mechanism this transformation was explored through control experiments and DFT calculations.
YKL-40, previously known as chitinase-3-like protein 1 (CHI3L1), is an inflammation-related glycoprotein that promotes atherosclerosis, but its application and optimal cut-off value a prognostic biomarker in coronary heart disease (CHD) require more clinical evidence. Thus, this prospective study aimed to evaluate the linkage of serum YKL-40 with features, inflammatory cytokines, major adverse cardiovascular events (MACEs) CHD patients.A total 410 patients were enrolled for determination via...