A5102965512- RNA and protein synthesis mechanisms
- Protein Structure and Dynamics
- Radar Systems and Signal Processing
- DNA and Nucleic Acid Chemistry
- Receptor Mechanisms and Signaling
- Microwave Imaging and Scattering Analysis
- Genomics and Phylogenetic Studies
- Bacteriophages and microbial interactions
- Advanced biosensing and bioanalysis techniques
- Advanced SAR Imaging Techniques
- vaccines and immunoinformatics approaches
- Photosynthetic Processes and Mechanisms
- Radio Wave Propagation Studies
- Ion Channels and Receptors
- Genetic Mapping and Diversity in Plants and Animals
- Lipid Membrane Structure and Behavior
- Quinazolinone synthesis and applications
- Biochemical and Structural Characterization
- Ubiquitin and proteasome pathways
- Ion channel regulation and function
- Chromosomal and Genetic Variations
- PI3K/AKT/mTOR signaling in cancer
- Microbial Metabolic Engineering and Bioproduction
- Metabolomics and Mass Spectrometry Studies
- Antimicrobial Peptides and Activities
Chinese University of Hong Kong
2025
Sir Run Run Shaw Hospital
2024
Zhejiang University
2024
Chinese University of Hong Kong, Shenzhen
2020-2024
University of Science and Technology of China
2021
Huazhong Agricultural University
2020
Harbin Institute of Technology
2018-2019
Ministry of Industry and Information Technology
2018
The last 18 months, or more, have seen a profound shift in our global experience, with many of us navigating once-in-100-year pandemic. To date, COVID-19 remains life-threatening pandemic little to no targeted therapeutic recourse. discovery novel antiviral agents, such as vaccines and drugs, can provide solutions save human beings from severe infections; however, there is specifically effective treatment confirmed for now. Thus, great attention has been paid the use natural artificial...
HMG-CoA reductase (HMGCR) protein is usually upregulated after statin (HMGCR inhibitor) treatment, which inevitably diminishes its therapeutic efficacy, provoking the need for higher doses associated with adverse effects. The proteolysis targeting chimera (PROTAC) technology has recently emerged as a powerful approach inducing degradation. Nonetheless, due to their bifunctional nature, developing orally bioavailable PROTACs remains great challenge. Herein, we identified HMGCR-targeted PROTAC...
Locating the low free energy paths (LFEPs) connecting different conformational states is among major tasks for simulations of complex biomolecules as pathways encode physical essence and, therefore, underlying mechanism their functional dynamics. Finding LFEPs yet challenging due to numerous degrees freedom molecules and expensive force calculations. To alleviate this issue, we have previously introduced a Traveling-Salesman-based Automated Path Searching (TAPS) approach that requires...
Constant false alarm rate (CFAR) is the desired property for automatic target detection in an unknown and non‐stationary background. Here, a modified cell averaging CFAR (CA‐CFAR) detector based on Grubbs criterion (CAG‐CFAR) proposed non‐homogeneous The of CAG‐CFAR with respect to distribution parameter exponential‐distributed background verified via Monte Carlo simulations. performances method scenarios multiple targets clutter edges are investigated different significance levels sizes...
The class C orphan G-protein-coupled receptor (GPCR) GPR156, which lacks the large extracellular region, plays a pivotal role in auditory function through Gi2/3. Here, we firstly demonstrate that GPR156 with high constitutive activity is essential for maintaining function, and further reveal structural basis of sustained GPR156. We present cryo-EM structures human apo GPR156–Gi3 complex, unveiling small region formed by loop 2 (ECL2) N-terminus. dimer both state Gi3 protein-coupled adopt...
Cytoplasmic male sterility (CMS) is a widely used genetic tool in modern hybrid rice breeding. Most genes conferring gametophytic CMS are homologous to
Human alkyladenine DNA glycosylase (AAG) is a key enzyme that corrects broad range of alkylated and deaminated nucleobases to maintain genomic integrity. When encountering the lesions, AAG adopts base-flipping strategy extrude target base from duplex its active site, thereby cleaving glycosidic bond. Despite functional importance, detailed mechanism such extrusion how distinguishes lesions an excess normal bases both remain elusive. Here, through Markov state model constructed on extensive...
Though crucial for understanding the function of large biomolecular systems, locating minimum free energy paths (MFEPs) between their key conformational states is far from trivial due to high-dimensional nature. Most existing path-searching methods require a static collective variable space as input, encoding intuition or prior knowledge transition mechanism. Such information is, however, hardly available priori and expensive validate. To alleviate this issue, we have previously introduced...
RNA secondary structures comprise double-stranded (ds) and single-stranded (ss) regions. Antisense peptide nucleic acids (asPNAs) enable the targeting of ssRNAs weakly formed dsRNAs. Nucleobase-modified dsRNA-binding PNAs (dbPNAs) allow for dsRNA targeting. A programmable RNA-structure-specific strategy is needed simultaneous recognition dsRNAs ssRNAs. Here, we report on combining dbPNAs asPNAs (designated as daPNAs) dsRNA-ssRNA junctions. Our data suggest that traditional asPNA (with a...
Bacteriophage T4 lysozyme (T4L) is a glycosidase that widely applied as natural antimicrobial agent in the food industry. Due to its wide applications and small size, T4L has been regarded model system for understanding protein dynamics large-scale engineering. Through structural insights from single conformation of T4L, series mutations (L99A,G113A,R119P) have introduced, which successfully raised fractional population only hydrolysis-competent excited state 96%. However, actual impact...
Constant false alarm rate (CFAR) is the desired property for automatic target detection in unknown and nonstationary background. In this paper, a modified cell averaging CFAR detector based on Grubbs criterion (CAG-CFAR) proposed multiple-target scenario, which encountered when two or more targets are displayed closely range domain. The of method with respect to distribution parameter exponential-distributed background verified via Monte Carlo simulations. CAG-CFAR does not require priori...
Summary RNA secondary structures comprise double-stranded (ds) and single-stranded (ss) regions. Antisense peptide nucleic acids (asPNAs) enable the targeting of ssRNAs weakly formed dsRNAs. Nucleobase-modified dsRNA-binding PNAs (dbPNAs) allow for relatively stable A programmable structure-specific strategy is needed simultaneous recognition dsRNAs ssRNAs. Here, we report on combining dbPNAs asPNAs (designated as daPNAs) dsRNA-ssRNA junctions. Our binding modeling data suggest that...
The structural basis for the allosteric interactions within G protein-coupled receptors (GPCRs) heterodimers remains largely unknown. metabotropic glutamate (mGlu) are complex dimeric GPCRs important fine tuning of many synapses. Heterodimeric mGlu with specific properties have been identified in brain. Here we report four cryo-electron microscopy structures mGlu2-4 heterodimer different states: an inactive state bound to antagonists, two intermediate states either mGlu2 or mGlu4 agonist...
Abstract The objective of this study was to explore the heterosis partial interspecific hybrids between African rice ( Oryza glaberrima Steud.) and Asian sativa L.) its correlation with genetic distance. Sixty‐nine accessions including 57 introgression lines (ILs) were tested for population structure Forty‐nine crosses ILs a test variety, Shengtai1 (ST1), evaluated midparent nine agronomic traits. Population structure, distance, proportion O. genome (PGG) estimated analyzed. PGG ranged from...
Transition state is a key concept for chemists to understand and fine-tune the conformational changes of large biomolecules. Due its short residence time, it difficult capture transition via experimental techniques. Characterizing states change therefore only achievable physics-driven molecular dynamics simulations. However, unlike chemical reactions which involve small number atoms, biomolecules depend on numerous atoms their coordinates in our 3D space. The searching will inevitably...
The significant wave height (SWH) is one of the most important ocean information describing sea state. high-precision SWH can be extracted using signal-to-noise ratio (SNR) method based on shore-based X-band radar. However, for ship-borne case, error increases because motion In this paper, effect radar vertical estimation analyzed in detail. First, images are simulated linear theory. Then, relationships between illumination rate and detection distance at different antenna heights discussed....