A5016535399- DNA Repair Mechanisms
- RNA modifications and cancer
- Neuroscience and Neuropharmacology Research
- Nitrogen and Sulfur Effects on Brassica
- ATP Synthase and ATPases Research
- Neuroinflammation and Neurodegeneration Mechanisms
- Cancer, Hypoxia, and Metabolism
- Ubiquitin and proteasome pathways
- Memory and Neural Mechanisms
- Lipid metabolism and biosynthesis
- Neonatal Respiratory Health Research
- Neural dynamics and brain function
- Asthma and respiratory diseases
- Autophagy in Disease and Therapy
- Cell Adhesion Molecules Research
- Neurogenesis and neuroplasticity mechanisms
- Receptor Mechanisms and Signaling
- Genetics and Neurodevelopmental Disorders
- Microtubule and mitosis dynamics
- Atherosclerosis and Cardiovascular Diseases
- Cell Image Analysis Techniques
- Tryptophan and brain disorders
- Advanced Fluorescence Microscopy Techniques
- Metabolomics and Mass Spectrometry Studies
- Nuclear Structure and Function
Chinese Institute for Brain Research
2017-2025
Peking University
2015-2025
State Key Laboratory of Membrane Biology
2017-2020
Children's Hospital of Chongqing Medical University
2018-2020
Chongqing Medical University
2018-2020
Chinese Academy of Medical Sciences & Peking Union Medical College
2020
Center for Life Sciences
2016-2019
Capital Medical University
2019
McGovern Institute for Brain Research
2018
National Health and Family Planning Commission
2018
Stimuli-controlled drug delivery and release is of great significance in cancer therapy, making a stimuli-responsive carrier highly demanded. Herein, multistimuli-controlled was developed by coating bovine serum albumin on Fe5C2 nanoparticles (NPs). With high loading the anticancer doxorubicin, nanoplatform provides burst when exposed to near-infrared (NIR) light or acidic conditions. In vitro experiment demonstrated NIR-regulated cell inhibition that ascribed from cellular uptake...
Herpes simplex virus type 1 strain 129 (H129) has represented a promising anterograde neuronal circuit tracing tool, which complements the existing retrograde tracers. However, current H129 derived tracers are multisynaptic, neither bright enough to label details of neurons nor capable determining direct projection targets as monosynaptic tracer.Based on bacterial artificial chromosome H129, we have generated serial recombinant viruses for tracing. Among them, H129-G4 was obtained by...
Transition metal copper (Cu) can exist in oxidized or reduced states cells, leading to cytotoxicity cancer cells through oxidative stress. Recently, complexes are emerging as a new class of anticancer compounds. Here, we report that novel complex (HYF127c/Cu) induces stress-dependent cell death cells. Further, transcriptional analysis revealed stress elicits broad changes genes, which autophagy-related genes significantly changed HYF127c/Cu-treated Consistently, autophagy was induced and...
The DNA damage response (DDR) is essential for the maintenance of genomic stability. Protein posttranslational modifications play pivotal roles in regulating DDR process. Here, we found that SUMOylated RNF168 undergoes liquid-liquid phase separation (LLPS), which restricts recruitment to sites, reduces RNF168-catalyzed H2A ubiquitination, restrains 53BP1 nuclear condensates, and ultimately impairs nonhomologous end joining repair efficiency. Sentrin/SUMO-specific protease 1 (SENP1) was...
Fragile X syndrome (FXS) is a neurodevelopmental disorder caused by mutations in the FMR1 gene that inactivate expression of product, fragile mental retardation 1 protein (FMRP). In this study, we used clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated 9 (Cas9) technology to generate Fmr1 knockout (KO) rats disruption fourth exon gene. Western blotting analysis confirmed FMRP was absent from brains KO (Fmr1exon4-KO ). Electrophysiological revealed...
Abstract Targeting the specific metabolic phenotypes of colorectal cancer stem cells (CRCSCs) is an innovative therapeutic strategy for (CRC) patients with poor prognosis and relapse. However, context-dependent traits CRCSCs remain poorly elucidated. Here we report that adenylate kinase hCINAP overexpressed in CRC tissues. Depletion inhibits invasion, self-renewal, tumorigenesis chemoresistance a loss mesenchymal signature. Mechanistically, binds to C-terminal domain LDHA, key regulator...
Abstract Serotonin is an important neurotransmitter that broadly participates in various biological processes. While serotonin deficiency has been associated with multiple pathological conditions such as depression, schizophrenia, Alzheimer’s disease and Parkinson’s disease, the serotonin-dependent mechanisms remain poorly understood. This study therefore aimed to identify novel biomarkers metabolic pathways perturbed by using metabolomics approach order gain new insights into...
Abstract Dysfunctions in ribosome biogenesis cause developmental defects and increased cancer susceptibility; however, the connection between assembly tumorigenesis remains unestablished. Here we show that hCINAP (also named AK6) is required for human 18S rRNA processing 40S subunit assembly. Homozygous CINAP −/− mice embryonic lethality. The heterozygotes are viable processing, whereas no delayed cell growth observed. However, during rapid growth, haploinsufficiency impairs protein...
Acute myeloid leukemia (AML) is a genetically heterogeneous malignant disorder of the hematopoietic system, characterized by accumulation DNA-damaged immature precursors. Here, we find that hCINAP involved in repair double-stranded DNA breaks (DSB) and its expression correlates with AML prognosis. Following DSB, recruited to damage sites where it promotes SENP3-dependent deSUMOylation NPM1. This turn results dissociation RAP80 from site CTIP-dependent resection homologous recombination. NPM1...
Abstract Background Quantifying the rich home‐cage activities of tree shrews provides a reliable basis for understanding their daily routines and building disease models. However, due to lack effective behavioral methods, most efforts on shrew behavior are limited simple measures, resulting in loss much information. Methods To address this issue, we present deep learning (DL) approach achieve markerless pose estimation recognize multiple spontaneous behaviors shrews, including drinking,...
<title>Abstract</title> Desmosomes are cell-cell adhesive junctions that provide structural integrity and mechanical resistance to tissues. Disruptions in desmosome organization lead severe cardiac dermatological disorders. Emerging evidence reveals desmosomes exhibit dynamic behaviors during remodeling. However, the mechanisms initiate regulate these dynamics under physiological conditions, their correlation with desmosome-related disorders remain elusive. Here, we uncovered role of...
Transforming growth factor-β (TGF-β) is known to promote breast cancer cell migration, invasion, and dissemination; however, the underlying molecular mechanisms are not yet well characterized. Here, we report that TGF-β induces pleckstrin-2 (PLEK2) expression by Smad3 signal transducer activator of transcription 3 (STAT3) activating PLEK2 promoter activity. Higher associated with poor prognosis in patients. Overexpression knockout experiments MDA-MB-231 MCF-7 cells revealed promotes...
Proper microtubule dynamics are critical for neuronal morphogenesis and functions, their dysregulation results in neurological disorders regeneration failure. Superior cervical ganglion-10 (SCG10, also known as stathmin-2 or STMN2) is a well-known regulator of neurons, but its functions the peripheral nervous system remain largely unknown. Here, we show that Scg10 knockout mice exhibit severely progressive motor sensory dysfunctions with significant sciatic nerve myelination deficits...
Highlights•Two-photon imaging enables the monitoring of neural activities on behaving animals•A neuronal subpopulation in mPFC that exhibited emergent properties encodes STM•These subpopulations exclusively comprise excitatory neurons•This STM coding mechanism is absent an animal model ADSummaryShort-term memory (STM) crucial for animals to hold information a small period time. Persistent or recurrent activity, together with oscillations, known encode at cellular level. However, mechanisms...
The 90-kDa heat shock protein HSP90AA1 is critical for the stability of several proteins that are important tumor progression and thus, a promising target cancer therapy. Selenosemicarbazone metal complexes have been shown to possess anticancer activity through an unknown molecular mechanism. MTT assay, fluorescence-activated cell sorting, fluorescent microscopy were used analyze mechanism anti-cancer selenosemicarbazone complexes. Additionally, RNA-seq was applied identify transcriptional...
Abstract Studies demonstrated that pneumonia can decrease vitamin A productions and reduction/deficiency may promote asthma development. Our previous study showed neonatal Streptococcus pneumoniae ( S. ) infection promoted Whether induced was associated with levels remains unclear. The aim of this to investigate the effects on expressions, explore supplement after adulthood Non-lethal established by intranasal inoculation (1-week-old) female BALB/c mice D39. were supplemented or without...
The centrosome composed of a pair centrioles (mother and daughter) pericentriolar material, is mainly responsible for microtubule nucleation anchorage in animal cells. subdistal appendage (SDA) centriolar structure located at the mother centriole’s region, it functions anchorage. However, molecular composition detailed SDA remain largely unknown. Here, we identified α-taxilin γ-taxilin as new components that form complex via their coiled-coil domains serve subgroup during hierarchical...
AMPA receptors (AMPARs) play a critical role in synaptic plasticity and learning memory, dysfunction or dysregulation of AMPARs could lead to various neurological psychiatric disorders, such as Alzheimer's disease (AD). However, the dynamics and/or longitudinal changes vivo during AD pathogenesis remain elusive. Here, employing 5xFAD SEP-GluA1 KI mice, we investigated endogenous receptor whisker deflection-associated Go/No-go paradigm. We found significant increase synaptosomal subunits...
Abstract Many people affected by fragile X syndrome (FXS) and autism spectrum disorders have sensory processing deficits, such as hypersensitivity to auditory, tactile, visual stimuli. Like FXS in humans, loss of Fmr1 rodents also cause sensory, behavioral, cognitive deficits. However, the neural mechanisms underlying impairment, especially vision remain unclear. It remains elusive whether deficits originate from corrupted inputs, impaired perception primary cortex, or altered integration...