A5101778063- Cancer-related molecular mechanisms research
- Protein Degradation and Inhibitors
- Ubiquitin and proteasome pathways
- Circular RNAs in diseases
- RNA modifications and cancer
- Topic Modeling
- Intelligent Tutoring Systems and Adaptive Learning
- Histone Deacetylase Inhibitors Research
- Peptidase Inhibition and Analysis
- Natural Language Processing Techniques
- Online Learning and Analytics
- Extracellular vesicles in disease
- Atherosclerosis and Cardiovascular Diseases
- Plant Gene Expression Analysis
- Cancer-related gene regulation
- MicroRNA in disease regulation
- Signaling Pathways in Disease
- Acoustic Wave Resonator Technologies
- Protease and Inhibitor Mechanisms
- Microbial Natural Products and Biosynthesis
- Reproductive System and Pregnancy
- Microwave Dielectric Ceramics Synthesis
- Epigenetics and DNA Methylation
- Horticultural and Viticultural Research
- Ferroelectric and Piezoelectric Materials
Ministry of Agriculture and Rural Affairs
2025
Chinese Academy of Agricultural Sciences
2025
Tea Research Institute
2025
Jiangsu University
2021-2025
Affiliated Hospital of Jiangsu University
2022-2025
Sichuan Agricultural University
2025
Nanjing Medical University
2025
Shaanxi Normal University
2024
Zhejiang University
2021-2024
University of Virginia
2020-2024
Abstract Background Premature ovarian insufficiency (POI) is an important cause of female infertility and seriously impacts the physical psychological health patients. Human umbilical cord mesenchymal stem cell-derived exosomes (HucMSCs-Exs, H-Exs) have exhibited protective effects on function with unclear mechanisms. Methods A comprehensive analysis Gene Expression Omnibus (GEO) database were used to identify POI-associated circRNAs miRNAs. The relationship between HucMSC-derived exosomal...
Central nervous system (CNS) immune privilege is complex and it still not understood how CNS antigens are sampled by the peripheral under steady state conditions. To compare antigen sampling from immune-privileged or nonprivileged tissues, we created transgenic mice with oligodendrocyte gut epithelial cell expression of an EGFP-tagged fusion protein containing ovalbumin (OVA) antigenic peptides tested anti-OVA peptide-specific sentinel OT-I OT-II T activation. We report that similarly, as...
Chemoresistance is a severe outcome among patients with epithelial ovarian cancer (EOC) that leads to poor prognosis. YBX-1 has been shown cause treatment failure and progression in EOC. However, strategies directly target are not yet conceivable. Here, we identified SIAH1 which was downregulated chemoresistant EOC samples cell lines functioned as novel E3 ligases trigger degradation of at cytoplasm by RING finger domain. Mechanistic studies show ubiquitinated lys304 lead the instability its...
Abstract Premature ovarian failure (POF) is an important cause of female infertility and seriously impacts the physical psychological health patients. Mesenchymal stromal cells-derived exosomes (MSCs-Exos) have essential role in treatment reproductive disorders, particularly POF. However, biological function therapeutic mechanism MSCs exosomal circRNAs POF remain to be determined. Here, with bioinformatics analysis functional assays, circLRRC8A was found downregulated senescent granulosa...
Intimal hyperplasia is a common cause of many vasculopathies. There has been recent surge interest in the bromo and extra-terminal (BET) epigenetic "readers" including BRD4 since serendipitous discovery JQ1(+), an inhibitor specific to seemingly undruggable BET bromodomains. The role family development intimal not known.We investigated effect inhibition on using rat balloon angioplasty model.While was dramatically up-regulated human hyperplastic neointima, blocking bromodomains with JQ1(+)...
Abstract Tumor-associated lncRNAs regulated by epigenetic modification switches mediate immune escape and chemoresistance in ovarian cancer (OC). However, the underlying mechanisms concrete targets have not been systematically elucidated. Here, we discovered that methylation modifications played a significant role regulating cell infiltration sensitizing OC to chemotherapy modulating immune-related (irlncRNAs), which represent tumor status. Through deep analysis of TCGA database, prognostic...
The treatment of black tea fermentation with different exogenous tannases was investigated, and processing parameters during fermentation, including time, temperature, exoenzyme amounts, were optimized, while the consumption transformation pathways catechins analyzed. results showed that tannase from Aspergillus niger ultimately selected as optimal enzyme to effectively increase content theaflavins by promoting hydrolysis reaction benzoylation catechins, resulting in a greater theaflavin...
Recently, interest in investigating the effects of long noncoding RNAs (lncRNAs) on endometrial receptivity (ER) has increased within field assisted reproductive technology. Therefore, objective this study is to identify and analyze role lncRNA LUCAT1 elucidate its specific mechanism regulating ER. Hub genes associated with ER are identified via Weighted gene co-expression network analysis (WGCNA) two datasets downloaded from GEO database. These hub WGCNA were subsequently validated. The...
The local tumor microenvironment (TME) of osteosarcoma (OS) includes several niches that control growth and cell extravasation. Migrasomes are recently discovered extracellular vesicles produced during migration. Herein, the results show OS production migrasomes in vivo vitro. Osteosarcoma cell-derived (OCDMs) aggravate proliferation metastasis, impeding OCDM formation alleviates malignant progression OS. Further studies revealed migrasome-associated nanoparticles (MANPs) functional unit...
Loquat fruit are susceptible to chilling injuries induced by postharvest storage at low temperature. The major symptoms increased lignin content and flesh firmness, which cause a leathery texture. Pretreatment with methyl jasmonate (MeJA) can alleviate this low-temperature-induced lignification, but the mechanism is not understood. In study, we characterized novel class III peroxidase, EjPRX12, studied its relationship lignification. Transcript levels of EjPRX12 were attenuated following...
Cellular plasticity is fundamental in biology and disease. Vascular smooth muscle cell (SMC) dedifferentiation (loss of contractile proteins) initiates perpetrates vascular pathologies such as restenosis. Contractile gene expression governed by the master transcription factor, serum response factor (SRF). Unlike other histone deacetylases, deacetylase 6 (HDAC6) primarily resides cytosol. Whether HDAC6 regulates SRF nuclear activity was previously unknown any type. This study found that...
Abstract Premature ovarian failure (POF) affects many adult women less than 40 years of age and leads to infertility. Mesenchymal stem cells-derived small extracellular vesicles (MSCs-sEVs) are attractive candidates for function restoration folliculogenesis POF due their safety efficacy, however, the key mediator in MSCs-sEVs that modulates this response underlying mechanisms remains elusive. Herein, we reported YB-1 protein was markedly downregulated vitro vivo models induced with H 2 O CTX...
Homo sapiens longevity assurance homologue 2 of yeast LAG1 (LASS2)/tumor metastasis suppressor gene 1 (TMSG1) was a novel tumor metastasis-related identified using messenger RNA differential display from non-metastatic human prostate cancer cell variants. The mechanism LASS2/TMSG1 inhibiting invasion in breast cells had not been well investigated. In the present study, full length 1.2 kb complementary DNA (cDNA) coding for protein 380 amino acids cloned. PcDNA3 eukaryotic expression plasmids...
Abstract CXCR4 is a stem/progenitor cell surface receptor specific for the cytokine stromal cell-derived factor-1 (SDF-1α). There evidence that bone marrow-derived CXCR4-expressing cells contribute to intimal hyperplasia (IH) by homing arterial subintima which enriched with SDF-1α. We have previously found transforming growth factor-β (TGFβ) and its signaling protein Smad3 are both upregulated following injury TGFβ/Smad3 enhances expression of in vascular smooth muscle (SMCs). It remains...
The bromodomain and extraterminal domain (BET) family proteins (BET2, BET3, BET4) “read” (bind) histone acetylation marks via two distinct bromodomains (Brom1 Brom2) facilitating transcriptional activation. These epigenetic “readers” play crucial roles in pathogenic processes such as inflammation. role of BETs influencing the degenerative process retina is however unknown. We employed rd10 mouse model (Pde6b mutation) retinitis pigmentosa (RP) to examine involvement BET retinal...
Abstract To date, there is no periadventitial drug delivery method available in the clinic to prevent restenotic failure of open vascular reconstructions. Resveratrol a promising anti-restenotic natural but subject low bioavailability when systemically administered. In order reconcile these two prominent issues, we tested effects resveratrol on all three major pro-restenotic pathologies including intimal hyperplasia (IH), endothelium impairment, and vessel shrinkage. rat carotid injury...
The sigma-2 receptor (S2R) has long been pharmacologically targeted for antipsychotic treatment and tumor imaging. Only recently was it known its coding gene role implicated in cholesterol homeostasis. Here, we have investigated the transcriptional control of S2R by Bromo/ExtraTerminal epigenetic reader family (BETs, including BRD2, 3, 4) upon perturbation. Cholesterol deprivation induced ARPE19 cells using a blocker lysosomal export. This condition up-regulated mRNA protein, also SREBP2 but...
Epigenetically switched, proliferative vascular smooth muscle cells (SMCs) form neointima, engendering stenotic diseases. Histone-3 lysine-27 trimethylation (H3K27me3) and acetylation (H3K27ac) marks are associated with gene repression activation, respectively. The polycomb protein embryonic ectoderm development (EED) reads H3K27me3 also enhances its deposition, hence is a canonical repressor. However, herein we found an unexpected role for EED in activating the