A5051145093- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Genetic and phenotypic traits in livestock
- Chromosomal and Genetic Variations
- Genomics and Phylogenetic Studies
- Genetic Mapping and Diversity in Plants and Animals
- Wildlife Ecology and Conservation
- Genetic diversity and population structure
- Sperm and Testicular Function
- Sexual Differentiation and Disorders
- Animal Diversity and Health Studies
- Bat Biology and Ecology Studies
- Botanical Research and Chemistry
- Animal Genetics and Reproduction
- Nicotinic Acetylcholine Receptors Study
- Venomous Animal Envenomation and Studies
- Biochemical and Structural Characterization
- Species Distribution and Climate Change
- Plant Taxonomy and Phylogenetics
- Cancer-related molecular mechanisms research
- Environmental DNA in Biodiversity Studies
- RNA and protein synthesis mechanisms
Texas A&M University
2017-2023
Duquesne University
2021-2022
Snakebite envenoming is a serious and neglected tropical disease that kills ~100,000 people annually. High-quality, genome-enabled comprehensive characterization of toxin genes will facilitate development effective humanized recombinant antivenom. We report de novo near-chromosomal genome assembly Naja naja, the Indian cobra, highly venomous, medically important snake. Our has scaffold N50 223.35 Mb, with 19 scaffolds containing 95% genome. Of 23,248 predicted protein-coding genes, 12,346...
The snow leopard, Panthera uncia, is an elusive high-altitude specialist that inhabits vast, inaccessible habitat across Asia. We conducted the first range-wide genetic assessment of leopards based on noninvasive scat surveys. Thirty-three microsatellites were genotyped and a total 683 bp mitochondrial DNA sequenced in 70 individuals. Snow exhibited low diversity at (AN = 5.8, HO 0.433, HE 0.568), virtually no mtDNA variation, underwent bottleneck Holocene (∼8000 years ago) coinciding with...
The development of high-quality chromosomally assigned reference genomes constitutes a key feature for understanding genome architecture species and is critical the discovery genetic blueprints traits biological significance. South American camelids serve people in extreme environments are important fiber companion animals worldwide. Despite this, alpaca lags far behind those available other domestic species. Here we produced chromosome-level improved assembly using DNA same female Huacaya...
LINE-1 is an active transposable element encoding proteins capable of inserting host gene retrocopies, resulting in retro-copy number variants (retroCNVs) between individuals. Here, we performed retroCNV discovery using 86 equids and identified 437 retrocopy insertions. Only 5 retroCNVs were shared horses other equids, indicating that the majority inserted after species diverged. A large (17–35 copies) segmentally duplicated Ligand Dependent Nuclear Receptor Corepressor Like ( LCORL )...
Disorders of sex development (DSD) and reproduction are not uncommon among horses, though knowledge about their molecular causes is sparse. Here we characterized a ~200 kb homozygous deletion in chromosome 29 at 29.7-29.9 Mb. The region contains AKR1C genes which function as ketosteroid reductases steroid hormone biosynthesis, including androgens estrogens. Mutations associated with human DSDs. Deletion boundaries, sequence properties gene content were studied by PCR whole genome sequencing...
We report 2 novel autosomal translocations in the horse. In Case 1, a breeding stallion with balanced t(4p;30) had produced normal foals and those congenital abnormalities. Of his 9 phenotypically offspring, 4 karyotypes, t(4p;30), 1 carried an unbalanced translocation tertiary trisomy of 4p. argue that forms are more tolerated result viable abnormalities, without causing embryonic death like all other known equine translocations. 2, two stallions by somatic cell nuclear transfer from same...
We generated single haplotype assemblies from a hinny hybrid which significantly improved the gapless contiguity for horse and donkey autosomal genomes X chromosomes. added over 15 Mb of missing sequence to both chromosomes, 60 autosomes corrected numerous errors in some reference genomes. resolved functionally important X-linked repeats: DXZ4 macrosatellite ampliconic Equine Testis Specific Transcript Y7 (ETSTY7). pinpointed location pseudoautosomal boundaries (PAB) determined size (1.8 Mb)...
A 7-year, purebred Gypsy Vanner, maiden mare was presented with abnormal cervical tissue that detected during prebreeding examination. External vulvar examination, transrectal and ultrasonographic examination of uterus, internal vaginal vault (via speculum endoscopy) were conducted. Karyotype analysis included sex chromosome study dual-color fluorescence in situ hybridization polymerase chain reaction for SRY gene. Mare had normal 64,XX karyotype no evidence mosaicism. Two distinct, patent,...
The unique evolutionary dynamics and complex structure make the Y chromosome most diverse least understood region in mammalian genome, despite its undisputable role sex determination, development, male fertility. Here we present first contig-level annotated draft assembly for alpaca (Vicugna pacos) based on hybrid of short- long-read sequence data flow-sorted Y. latter was also used cDNA selection providing Y-enriched testis transcriptome annotation. final 8.22 Mb comprised 4.5 specific...
A 10-year-old intact female Chinese Crested dog was presented for evaluation and further diagnostics due to persistent symptoms of vulvar swelling, vaginal discharge, an 8-year history acyclicity. At presentation, generalized hyperpigmentation truncal alopecia were identified, with no aberrations the phenotype. Vaginal cytology confirmed influence estrogen at multiple veterinary visits, hormonal screening progesterone anti-Mullerian hormone indicated gonadal presence. Based on findings from...