A5011662443- Reproductive Physiology in Livestock
- Reproductive System and Pregnancy
- Veterinary Equine Medical Research
- T-cell and B-cell Immunology
- Genetic and phenotypic traits in livestock
- Immune Cell Function and Interaction
- Monoclonal and Polyclonal Antibodies Research
- Genetic Mapping and Diversity in Plants and Animals
- Pregnancy and preeclampsia studies
- Reproductive Biology and Fertility
- Immunotherapy and Immune Responses
- Immune Response and Inflammation
- Animal health and immunology
- Glycosylation and Glycoproteins Research
- Genetic diversity and population structure
- Microbial infections and disease research
- T-cell and Retrovirus Studies
- Mesenchymal stem cell research
- Herpesvirus Infections and Treatments
- Veterinary Oncology Research
- Animal Disease Management and Epidemiology
- RNA and protein synthesis mechanisms
- Mast cells and histamine
- Cell Adhesion Molecules Research
- Molecular Biology Techniques and Applications
Cornell University
2015-2024
New York State College of Veterinary Medicine
1991-2016
Ithaca College
2014
The Centers
2011
Centers for Disease Control and Prevention
2011
Louisiana State University Health Sciences Center New Orleans
2011
Syracuse University
2010
Texas A&M University
2003
Département Génétique Animale
2003
University of California, Davis
1987-2003
A Horse Is a Horse, of Course The history horse domestication is closely tied to the human society. Wade et al. (p. 865 ) report on sequencing and provide single nucleotide polymorphism map ( Equus caballus genome. Horses are member order perissodactyla (odd-toed animals with hooves). analysis reveals an evolutionarily new centromere equine chromosome 11 that displays properties immature but fully functioning devoid centromeric satellite sequence. findings clarify nature genetic diversity...
Significance Thirty years after the first DNA fragment from extinct quagga zebra was sequenced, we set another milestone in equine genomics by sequencing its entire genome, along with genomes of surviving species. This extensive dataset allows us to decipher genetic makeup underlying lineage-specific adaptations and reveal complex history speciation. We find that Equus diverged New World, spread across Old World 2.1–3.4 Mya, finally experienced major demographic expansions collapses...
Recent advances in genomic sequencing technology and computational assembly methods have allowed scientists to improve reference genome assemblies terms of contiguity composition. EquCab2, a for the domestic horse, was released 2007. Although equal or better quality compared other first-generation Sanger assemblies, it had many shortcomings common them. In 2014, equine genomics research community began project sequence building upon solid foundation EquCab2 incorporating new short-read data,...
The discovery of genomic imprinting through studies manipulated mouse embryos indicated that the paternal genome has a major influence on placental development. However, previous research not demonstrated bias in imprinted genes. We applied RNA sequencing to trophoblast tissue from reciprocal hybrids horse and donkey, where genotypic differences allowed parent-of-origin identification most expressed Using this approach, we identified core group 15 ancient genes, which 10 were paternally...
The horse is a valuable species to assess the effect of allogeneic mesenchymal stromal cells (MSCs) in regenerative treatments. No studies date have examined recipient response major histocompatibility complex (MHC)-mismatched equine MSCs. purposes this study were immunophenotype MSCs from horses known MHC haplotype and compare immunogenicity with differing class II expression.MSCs peripheral blood leukocytes (PBLs) obtained Thoroughbred (n=10) (ELA-A2, -A3, -A9 homozygotes). cultured...
Abstract Introduction This study tested the hypothesis that Major Histocompatibility Complex (MHC) incompatible equine mesenchymal stromal cells (MSCs) would induce cytotoxic antibodies to donor MHC antigens in recipient horses after intradermal injection. No studies date have explored antibody responses allogeneic MSC transplantation horse. information is critical because horse a valuable species for assessing safety and efficacy of treatment prior human clinical application. Methods Six...
Abstract The Arabian horse, one of the world’s oldest breeds any domesticated animal, is characterized by natural beauty, graceful movement, athletic endurance, and, as a result its development in arid Middle East, ability to thrive hot, dry environment. Here we studied 378 horses from 12 countries using equine single nucleotide polymorphism (SNP) arrays and whole-genome re-sequencing examine hypotheses about genomic diversity, population structure, relationship other horse breeds. We...
A first-generation radiation hybrid (RH) map of the equine (Equus caballus) genome was assembled using 92 horse x hamster cell lines and 730 markers. The is first comprehensive framework that (1) incorporates type I as well II markers, (2) integrates synteny, cytogenetic, meiotic maps into a consensus map, (3) provides most detailed genome-wide information to date on organization comparative status genome. loci (258 472 II) included in final are clustered 101 RH groups distributed over all...
The final assignment of antibody clusters for leucocyte antigens and immunoglobulins, as described in detail Sections 3 4, is summarized Table 4. Together with other mAbs developed outside ELAW II (Table 9) this pool reagents represent a powerful array tools the study equine immunity. Second Equine Leucocyte Antigen Workshop made considerable advances pursuing objectives establishing specificities achieving consensus on nomenclature immunoglobulin molecules. Of equal importance, several...
Lavender Foal Syndrome (LFS) is a lethal inherited disease of horses with suspected autosomal recessive mode inheritance. LFS has been primarily diagnosed in subgroup the Arabian breed, Egyptian horse. The condition characterized by multiple neurological abnormalities and dilute coat color. Candidate genes based on comparative phenotypes mice humans include ras-associated protein RAB27a (RAB27A) myosin Va (MYO5A). Here we report mapping locus responsible for using small set 36 segregating...
Summary. The relationship between breed and the risk of developing sarcoid tumours or uveitis unknown etiology was evaluated in a retrospective study 16242 equine cases admitted 1975 1987 to New York State College Veterinary Medicine Large Animal Hospital, 3198 tissue samples sent Diagnostic Laboratory 1977 1987. Of 120 from sarcoids were twice as likely develop Quarter Horses (odds ratio, OR=1.8, P <0.05) relative Thoroughbreds less than half Standardbreds (OR=0.2, <0.001)...
Mammalian centromeres are associated with highly repetitive DNA (satellite DNA), which has so far hindered molecular analysis of this chromatin domain. Centromeres epigenetically specified, and binding the CENPA protein is their main determinant. In previous work, we described first example a natural satellite-free centromere on Equus caballus Chromosome 11. Here, investigated asinus by using ChIP-seq anti-CENPA antibodies. We identified an extraordinarily high number lacking satellite (16...
Abstract This report contains the first map of complete Ig H chain constant (IGHC) gene region horse (Equus caballus), represented by 34 overlapping clones from a new bacterial artificial chromosome library. The different inserts containing IGHC genes were identified and arranged hybridization using overgo probes specific for individual equine genes. analysis these two previously undetected horse. newly found IGHG7 gene, which has high homology to IGHG4 is located between IGHG3 degree...